PHGDH (Myc-DDK-tagged)-Human phosphoglycerate dehydrogenase (PHGDH)
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
Products
Recombinant protein of human phosphoglycerate dehydrogenase (PHGDH), full length, with C-terminal DDK tag, expressed in sf9, 20ug
| Tag | C-DDK |
| Expression Host | Sf9 |
PHGDH - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pGFP-V-RS |
PHGDH - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
Recombinant protein of human phosphoglycerate dehydrogenase (PHGDH), 100 µg
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
Recombinant protein of human phosphoglycerate dehydrogenase (PHGDH), 1 mg
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
PHGDH - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
Recombinant protein of human phosphoglycerate dehydrogenase (PHGDH), full length, with N-terminal HIS tag, expressed in sf9, 20ug
| Tag | N-His |
| Expression Host | Sf9 |
Transient overexpression lysate of phosphoglycerate dehydrogenase (PHGDH)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
PHGDH - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
PHGDH CRISPRa kit - CRISPR gene activation of human phosphoglycerate dehydrogenase
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
PHGDH - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRFP-C-RS |
qPCR primer pairs and template standards against Homo sapiens gene PHGDH
| Application | Plasmid of exact quantity for transcript copy number calculation |
qSTAR qPCR primer pairs against Homo sapiens gene PHGDH
Transient overexpression of PHGDH in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
