Purified recombinant protein of Homo sapiens protein phosphatase 1D magnesium-dependent, delta isoform (PPM1D), 20 µg
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
Products
PPM1D (Myc-DDK-tagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1D (PPM1D)
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
PPM1D - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pGFP-V-RS |
PPM1D (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
Transient overexpression lysate of protein phosphatase 1D magnesium-dependent, delta isoform (PPM1D)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
PPM1D - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
Purified recombinant protein of Homo sapiens protein phosphatase 1D magnesium-dependent, delta isoform (PPM1D), 100 µg
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
Purified recombinant protein of Homo sapiens protein phosphatase 1D magnesium-dependent, delta isoform (PPM1D), 1 mg
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
PPM1D - human gene knockout kit via CRISPR, HDR mediated
| Format | 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control |
| Donor DNA | GFP-puro |
PPM1D CRISPRa kit - CRISPR gene activation of human protein phosphatase, Mg2+/Mn2+ dependent 1D
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
PPM1D - human gene knockout kit via CRISPR, HDR mediated
| Format | 2 gRNA vectors, 1 mBFP-Neo donor, 1 scramble control |
| Donor DNA | mBFP-Neo |
PPM1D - human gene knockout kit via CRISPR, HDR mediated
| Format | 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control |
| Donor DNA | RFP-BSD |
PPM1D - human gene knockout kit via CRISPR, HDR mediated
| Format | 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control |
| Donor DNA | Luciferase-Puro |
qSTAR qPCR primer pairs against Homo sapiens gene PPM1D
qPCR primer pairs and template standards against Homo sapiens gene PPM1D
| Application | Plasmid of exact quantity for transcript copy number calculation |
