PYGL (Myc-DDK-tagged)-Human phosphorylase, glycogen, liver (PYGL), transcript variant 1
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
Products
PYGL (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
PYGL - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
PYGL (Myc-DDK-tagged)-Human phosphorylase, glycogen, liver (PYGL), transcript variant 2
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
- TrueORF®
Recombinant protein of human phosphorylase, glycogen, liver (PYGL), 1 mg
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
Recombinant protein of human phosphorylase, glycogen, liver (PYGL), 100 µg
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
Transient overexpression lysate of phosphorylase, glycogen, liver (PYGL), transcript variant 1
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
PYGL HEK293T cell transient overexpression lysate (as WB positive control)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
PYGL CRISPRa kit - CRISPR gene activation of human glycogen phosphorylase L
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
qSTAR qPCR primer pairs against Homo sapiens gene PYGL
qPCR primer pairs and template standards against Homo sapiens gene PYGL
| Application | Plasmid of exact quantity for transcript copy number calculation |
Transient overexpression of PYGL, transcript variant 1, in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
PYGL - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
PYGL - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Recombinant protein of human phosphorylase, glycogen, liver (PYGL), 20 µg
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
