RFNG (Myc-DDK-tagged)-Human RFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (RFNG)
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
- TrueORF®
Products
RFNG - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
RFNG CRISPRa kit - CRISPR gene activation of human RFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
RFNG HEK293T cell transient overexpression lysate (as WB positive control)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
qPCR primer pairs and template standards against Homo sapiens gene RFNG
| Application | Plasmid of exact quantity for transcript copy number calculation |
qSTAR qPCR primer pairs against Homo sapiens gene RFNG
RFNG - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
RFNG - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
RFNG (tGFP-tagged) - Human RFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (RFNG)
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
- TrueORF®
Lenti-ORF clone of RFNG (mGFP-tagged)-Human RFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (RFNG)
| Type | Human Tagged ORF Clone |
| Tag | mGFP |
| Vector | pLenti-C-mGFP-P2A-Puro |
- LentiORF®
Lenti-ORF clone of RFNG (Myc-DDK-tagged)-Human RFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (RFNG)
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
- LentiORF®
RFNG (untagged)-Human RFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (RFNG)
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL4 |
RFNG (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
SR321609 is the updated version of SR304044.
3' UTR clone of RFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (RFNG) for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
Transient overexpression lysate of RFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (RFNG)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
