Products

Purified recombinant protein of Human topoisomerase (DNA) I (TOP1),Lys93-Lys151, with N-terminal His-ABP tag, expressed in E. coli, 50ug

Tag N-His-ABP (Albumin-Binding Protein)
Expression Host E. coli

TOP1 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.

Format 2 gRNA vectors, 1 linear donor
Donor DNA EF1a-GFP-P2A-Puro

TOP1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided

Format Retroviral plasmids
Vector pRS

TOP1 HEK293T cell transient overexpression lysate (as WB positive control)

Tag C-Myc/DDK
Expression Host HEK293T

3' UTR clone of topoisomerase (DNA) I (TOP1) for miRNA target validation

Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase