USE1 (Myc-DDK-tagged)-Human unconventional SNARE in the ER 1 homolog (S. cerevisiae) (USE1)
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
Products
USE1 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
Purified recombinant protein of Human unconventional SNARE in the ER 1 homolog (S. cerevisiae) (USE1), full length, with N-terminal GST and C-terminal HIS tag, expressed in E. coli, 50ug
| Tag | N-GST and C-His |
| Expression Host | E. coli |
USE1 CRISPRa kit - CRISPR gene activation of human unconventional SNARE in the ER 1
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Transient overexpression lysate of unconventional SNARE in the ER 1 homolog (S. cerevisiae) (USE1)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
qPCR primer pairs and template standards against Homo sapiens gene USE1
| Application | Plasmid of exact quantity for transcript copy number calculation |
qSTAR qPCR primer pairs against Homo sapiens gene USE1
Transient overexpression of USE1 in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
USE1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
USE1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
USE1 (tGFP-tagged) - Human unconventional SNARE in the ER 1 homolog (S. cerevisiae) (USE1)
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
- TrueORF®
Lenti ORF clone of Human unconventional SNARE in the ER 1 homolog (S. cerevisiae) (USE1), mGFP tagged
| Type | Human Tagged ORF Clone |
| Tag | mGFP |
| Vector | pLenti-C-mGFP-P2A-Puro |
- LentiORF®
Lenti ORF clone of Human unconventional SNARE in the ER 1 homolog (S. cerevisiae) (USE1), Myc-DDK-tagged
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
- LentiORF®
USE1 HEK293T cell transient overexpression lysate (as WB positive control)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
USE1 (untagged)-Human unconventional SNARE in the ER 1 homolog (S. cerevisiae) (USE1)
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-AC |
