Isy1 - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
Products
Isy1 (Myc-DDK-tagged) - Mouse ISY1 splicing factor homolog (S. cerevisiae) (Isy1)
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
- TrueORF®
qPCR primer pairs and template standards against Mus musculus gene 5830446M03Rik
| Application | Plasmid of exact quantity for transcript copy number calculation |
qSTAR qPCR primer pairs against Mus musculus gene Isy1
Isy1 - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
Isy1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Isy1 (tGFP-tagged) - Mouse ISY1 splicing factor homolog (S. cerevisiae) (Isy1)
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
- TrueORF®
Lenti ORF clone of Isy1 (mGFP-tagged) - Mouse ISY1 splicing factor homolog (S. cerevisiae) (Isy1)
| Type | Mouse Tagged ORF Clone |
| Tag | mGFP |
| Vector | pLenti-C-mGFP-P2A-Puro |
- LentiORF®
Lenti ORF clone of Isy1 (Myc-DDK-tagged) - Mouse ISY1 splicing factor homolog (S. cerevisiae) (Isy1)
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
- LentiORF®
Isy1 (untagged) - Mouse ISY1 splicing factor homolog (S. cerevisiae) (Isy1), (10ug)
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Vector | PCMV6-Kan/Neo |
Isy1 (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
