Plin5 (NM_001077348) Mouse Tagged ORF Clone
CAT＃: MR207402
- TrueORF®
Plin5 (Myc-DDK-tagged) - Mouse perilipin 5 (Plin5), transcript variant 2
ORF Plasmid: tGFP
"NM_001077348" in other vectors (3)
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CNY 3656.00
Product images
CNY 6840.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2310076L09Rik; AI415325; AW109675; Lsdp5; MLDP; PAT-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207402 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCAGAGAGGTGAAGACACCACCCTAGCGCCACACAGCAGAATGTCCGGTGATCAGACAGCTCAGG ACCCTGGATCCAGCCTGGGAGAACTGGACCAGCAGAATGTGGTGAATCGAGTGGTGGCTTTGCCCCTGGT CAAGGCCACGTGCACTGCCGTGTCCAGTGCTTACAACTCGGCCAAGGACAGGCACCCGCTGCTGGGCTCC GCCTGCCGCCTTGCTGAGCACTGTGTGTGTAGTGTGACTACCTGTGCCCTGGACCACGCACAGCCACTGC TGGAGCACCTGCAGCCCCAGTTGGCCACAGTGAATGATCTTGCCTGCAGGGGACTAGACAAATTGGAAGA GAAGCTGCCCTTCCTGCAGCAGCCATCAGACATGGTGGTGACATCAGCCAAGGATACAGTGGCCAAAAGT GTCACAGGCATGGTGGACCTGGCCCAAAGGGGCCGGCGTTGGAGTGGGGAGCTGAGGCGCTCCATGAGTC AAGCCATGGACATGGTGCTGGGCAAGTCGGAGAAGCTGGTGGACCGCTTCCTGCCCATGACTGAGGCTGA GCTAGCAGTCCTGGCAGCTGAGGCCGAGGGCCCAGAAGTGGGCACAGTGGAGGAGCAGAGGCAGCAACAG GGCTACTTTGTGCGTCTGGGGTCCCTATCGGCACGCCTCCGCCATCTCGCCTATGAACACTCTTTGGGGA AACTGAGGCAGAGCAAACACCGTACCCAGGAGATGCTGGCCCAGCTGCAGGAAACGCTGGAGCTGATCCA GCATATGCAGAGAGGGGCAAGCCCTAGCCCTACTTTCCATCCCCCAAAGACTCAGGAGCTGTGGGGGAGC TGGAGCCCGTGTCTAGAGAATGGCCGCAGCCACAGTGAGGTGGAGCTGGAGACACTGGCTCTGTCTCGAA GTTTGACCCTGGAGCTGCAGAATGCAGTGGATGCCCTGGCAGGCTGTGTTCGGGGCCTGCCACCTAGTGC CCAGGCCAAGGTGGCTGAGGTGCAGCGCAGCGTGGATGCTCTACAGGCCACCTTTGCTGATGCACACTGC CTTGGTGATGTGGCACCCACTGCTCTGGCTGAGGGCCGGGGCAGTGTGGCCCGGGCACATGCCTGTGTGG ATGAGTTCCTGGATTTGGTCCTGCGGGCCATGCCACTGCCCTGGCTTGTGGGGCCCTTTGCACCCATCCT GGTGGAACAGTCGGAGCCCCTGATCAACCTGGCCACCTGTGTGGACGAGGTGGTGGGTGACCCTGATCCT CGCTGGGCACACATGGACTGGCCAGCCCAGAAGAGGGCCTGGGAGGCTGAGTCTGCAGATCCTGGGGGGC AAGAGGCTGAGCCCCCAAGGGGGCAAGGCAAGCACACAATGATGCCAGAGCTGGACTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207402 protein sequence
Red=Cloning site Green=Tags(s) MDQRGEDTTLAPHSRMSGDQTAQDPGSSLGELDQQNVVNRVVALPLVKATCTAVSSAYNSAKDRHPLLGS ACRLAEHCVCSVTTCALDHAQPLLEHLQPQLATVNDLACRGLDKLEEKLPFLQQPSDMVVTSAKDTVAKS VTGMVDLAQRGRRWSGELRRSMSQAMDMVLGKSEKLVDRFLPMTEAELAVLAAEAEGPEVGTVEEQRQQQ GYFVRLGSLSARLRHLAYEHSLGKLRQSKHRTQEMLAQLQETLELIQHMQRGASPSPTFHPPKTQELWGS WSPCLENGRSHSEVELETLALSRSLTLELQNAVDALAGCVRGLPPSAQAKVAEVQRSVDALQATFADAHC LGDVAPTALAEGRGSVARAHACVDEFLDLVLRAMPLPWLVGPFAPILVEQSEPLINLATCVDEVVGDPDP RWAHMDWPAQKRAWEAESADPGGQEAEPPRGQGKHTMMPELDF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001077348 |
| ORF Size | 1389 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq Size | 1994 bp |
| RefSeq ORF | 1392 bp |
| Locus ID | 66968 |
| UniProt ID | Q8BVZ1 |
| MW | 50.5 kDa |
| Gene Summary | Lipid droplet-associated protein that maintains the balance between lipogenesis and lipolysis and also regulates fatty acid oxidation in oxidative tissues. Recruits mitochondria to the surface of lipid droplets and is involved in lipid droplet homeostasis by regulating both the storage of fatty acids in the form of triglycerides and the release of fatty acids for mitochondrial fatty acid oxidation. In lipid droplet triacylglycerol hydrolysis, plays a role as a scaffolding protein for three major key lipolytic players: ABHD5, PNPLA2 and LIPE. Reduces the triacylglycerol hydrolase activity of PNPLA2 by recruiting and sequestering PNPLA2 to lipid droplets. Phosphorylation by PKA enables lipolysis probably by promoting release of ABHD5 from the perilipin scaffold and by facilitating interaction of ABHD5 with PNPLA2. Also increases lipolysis through interaction with LIPE and upon PKA-mediated phosphorylation of LIPE.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG207402 | Plin5 (tGFP-tagged) - Mouse RIKEN cDNA 2310076L09 gene (2310076L09Rik), transcript variant 2 |
CNY 3710.00 |
|
| MR207402L3 | Lenti ORF clone of Plin5 (Myc-DDK-tagged) - Mouse perilipin 5 (Plin5), transcript variant 2 |
CNY 6056.00 |
|
| MR207402L4 | Lenti ORF clone of Plin5 (mGFP-tagged) - Mouse perilipin 5 (Plin5), transcript variant 2 |
CNY 6056.00 |
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
