GAB3 (NM_001282283) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336742 representing NM_001282283. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGTGCGGGCGACGCAGTGTGCACCGGCTGGCTCGTTAAGTCGCCCCCCGAGAGGAAGCTACAGCGC TACGCCTGGCGCAAGCGCTGGTTTGTCCTCCGGCGAGGCCGCATGAGCGGCAACCCCGATGTCTTGGAG TACTACAGGAACAAGCACTCCAGCAAGCCCATCCGGGTGATAGACCTCAGCGAGTGTGCAGTGTGGAAG CATGTGGGCCCCAGCTTTGTTCGGAAGGAATTTCAGAATAATTTCGTGTTCATTGTCAAGACTACTTCC CGTACATTCTACCTGGTGGCCAAAACTGAGCAAGAAATGCAGGTGTGGGTGCACAGCATCAGTCAGGTC TGCAACCTTGGCCACCTGGAGGATGGTGCAGCAGATTCCATGGAGAGCCTCTCTTACACGCCCTCCTCC CTGCAGCCATCCTCTGCCAGCTCCCTTCTTACCGCCCATGCTGCCAGCTCCTCTTTGCCAAGAGATGAC CCAAACACTAATGCCGTAGCCACTGAGGAAACCAGAAGTGAGTCAGAGCTTCTCTTCCTTCCAGATTAT CTGGTTTTGTCCAACTGCGAGACTGGAAGACTGCACCATACCAGTCTACCCACCAGATGTGATAGCTGG TCAAACTCAGACCGTTCATTGGAACAGGCTTCATTTGATGATGTTTTTGTTGACTGCCTGCAGCCGCTC CCCTCCAGTCATTTGGTCCACCCCTCATGCCATGGCAGTGGAGCTCAGGAGGTGCCATCCTCGAGGCCT CAGGCTGCCCTGATCTGGAGTAGAGAAATCAATGGGCCACCCAGGGACCACTTGTCTTCTTCACCATTG CTGGAAAGTTCCTTAAGTTCCACCATTCAGGTAGATAAAAATCAAGGTTCCTTACCCTGTGGAGCAAAA GAACTAGACATTATGTCCAACACTCCACCTCCCCGCCCCCCTAAGCCAAGCCATCTGTCTGAACGGCGC CAAGAGGAGTGGAGTACACACAGTGGTAGCAAGAAGCCAGAATGCACTCTGGTTCCAAGAAGAATCTCC CTCTCTGGTTTAGACAACATGAGAACCTGGAAAGCTGATGTAGAAGGCCAATCCTTAAGACACCGAGAC AAGCGGCTTAGTTTGAATTTGCCATGCAGGTTCTCCCCGATGTACCCCACAGCTTCAGCCAGTATCGAA GACAGCTATGTGCCCATGAGCCCCCAGGCTGGTGCCTCTGGTCTTGGACCCCACTGCAGCCCTGATGAC TACATTCCAATGAACTCAGGAAGCATCTCAAGCCCGTTGCCTGAGCTGCCTGCAAACCTGGAACCTCCC CCAGTGAATAGAGATCTCAAGCCTCAGAGGAAATCACGGCCACCTCCTCTGGACCTGAGAAACCTCTCG ATCATCCGGGAACATGCATCTCTTACCAGGACCCGCACTGTGCCTTGCAGTCGAACCAGCTTTCTCTCT CCAGAAAGAAATGGTATTAATTCTGCAAGATTTTTTGCTAATCCTGTTTCCAGAGAAGACGAAGAAAGC TACATCGAAATGAAACTTCTCCTTTCAGAAGAACAAAGAGTAGACTATGTCCAAGTGGATGAGCAGAAG ACACAGGCTCTCCAGAGCACAAAACAGGAGTGGACGGATGAAAGGCAATCCAAAGTATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001282283
Insert Size	1647 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282283.1
RefSeq Size	4647 bp
RefSeq ORF	1647 bp
Locus ID	139716
UniProt ID	Q8WWW8
MW	61.4 kDa
Gene Summary	This gene is a member of the GRB2-associated binding protein gene family. These proteins are scaffolding/docking proteins that are involved in several growth factor and cytokine signaling pathways, and they contain a pleckstrin homology domain, and bind SHP2 tyrosine phosphatase and GRB2 adapter protein. The protein encoded by this gene facilitates macrophage differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013] Transcript Variant: This variant (3) lacks an in-frame exon in the 3' coding region, compared to variant 1. The encoded isoform (3) is shorter, compared to isoform 1.

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