SARS-CoV-2 ORF7a Gene Tagged ORF (codon optimized)

CAT#: VC102561

  • TrueORF®

Myc-DDK-tagged ORF clone for SARS-CoV-2 ORF7a protein [Severe acute respiratory syndrome coronavirus 2], codon optimized for human cell expression, YP_009724395



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CNY 3,800.00


货期*
3周

规格
    • 10 ug

Product images

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Specifications

Product Data
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC102561 represents NCBI reference of YP_009724395 with codon optimized for human cell expression
Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGAAGATCATTCTGTTTCTGGCTCTGATCACACTGGCAACCTGTGAACTCTATCATTACCAAGAATGC
GTAAGAGGAACAACAGTGTTGTTGAAAGAACCTTGTTCATCTGGTACGTATGAGGGAAATTCACCATTC
CATCCCCTGGCCGATAATAAATTCGCCTTGACATGTTTCTCCACCCAATTCGCCTTCGCATGCCCAGAC
GGAGTAAAACACGTATATCAGCTGAGAGCTAGATCCGTTAGCCCGAAACTTTTTATAAGACAAGAGGAG
GTCCAAGAACTGTATAGTCCAATCTTTCTGATAGTAGCCGCTATTGTATTTATCACTCTGTGTTTCACT
CTCAAAAGAAAAACCGAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
>VC102561 representing YP_009724395
Blue=ORF Red=Cloning site Green=Tag(s)

MKIILFLALITLATCELYHYQECVRGTTVLLKEPCSSGTYEGNSPFHPLADNKFALTCFSTQFAFACPD
GVKHVYQLRARSVSPKLFIRQEEVQELYSPIFLIVAAIVFITLCFTLKRKTE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_045512
ORF Size 363 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NC_045512.2, YP_009724395
RefSeq ORF 363 bp
MW 13.7 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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