ERCC1 Mouse Monoclonal Antibody [Clone ID: 8F1]

Specifications

Product Data
Clone Name	8F1
Applications	IHC, IP, WB
Recommend Dilution	Immunoprecipitation. Immunohistochemistry on Paraffin Embedded Sections: 1/100-1/200 in an ABC method. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to Immunostaining. Incubation Time: 30 minutes at RT. Recommended Positive Control: Tonsil. Western Blot: 1/100-1/500. use Human lung cell line as Positive Control.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	BALB/C mice were injected with full length recombinant human ERCC1 protein.
Specificity	This antibody reacts with a 33-36 kDa protein known as ERCC1 polypeptide. Cellular localization: Nuclear.
Formulation	State: Purified State: Liquid purified IgG fraction Preservative: < 0.1% Sodium Azide
Purification	Protein A Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	ERCC excision repair 1, endonuclease non-catalytic subunit
Database Link	Entrez Gene 2067 Human UniProt ID P07992
Background	The mammalian ERCC1 (Excision Repair Cross Complementing) polypeptide is required for nucleotide excision repair (NER) of damaged DNA and is homologous to Saccharomyces cerevisiae RAD10, which functions in repair and mitotic intrachromosomal recombination. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells XPG cleaves 3' of the DNA lesion while the ERCC1-XPF complex makes the 5' incision. Elevated levels of ERCC1 have also been reported in Cisplatin-resistant cells.
Synonyms	DNA excision repair protein ERCC-1, COFS4, UV20
Note	Molecular weight of antigen :33-36 kDa
Reference Data

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