HIF-1 alpha (HIF1A) Rabbit Polyclonal Antibody

CAT#: TA336260

Rabbit Polyclonal HIF-1 alpha Antibody



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CNY 6,056.00


货期*
5周

规格
    • 100 ul

经常一起买 (2)
Transient overexpression lysate of hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant 2
    • 100 ug

CNY 4,840.00


beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control
    • 30 ul

CNY 300.00
CNY 1,430.00

Specifications

Product Data
Applications ChIP, ELISA, ICC/IF, IHC, Immunoblotting, IP, Simple Western, WB
Recommend Dilution Dual RNAscope ISH-IHC, Chromatin Immunoprecipitation (ChIP), Western Blot: 1:500 - 1:1000, Simple Western: 1:100, Gel Super Shift Assays: 1:1 - 1:100, Immunohistochemistry: 1:100 - 1:500, Immunohistochemistry-Paraffin: 1:100 - 1:500, Immunohistochemistry-Frozen: 1:100 - 1:500, Immunoprecipitation: 1:1000, Immunocytochemistry/ Immunofluorescence, ELISA, Immunoblotting, Chromatin Immunoprecipitation Sequencing, Knockout Validated
Reactivity Human, Mouse, Rat, Canine, Guinea Pig, Primate, Xenopus
Host Rabbit
Clonality Polyclonal
Immunogen Fusion protein made to an internal sequence of human HIF-1 alpha (containing amino acids 432-528) [UniProt Q16665]
Formulation PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration lot specific
Purification Immunogen affinity purified
Conjugation Unconjugated
Storage Condition Store at -20°C as received.
Predicted Protein Size 120 kDa
Gene Name hypoxia inducible factor 1 alpha subunit
Background HIF-1 alpha (HIF1A) is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell.
Synonyms bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8
Note This HIF-1 alpha antibody is useful for Chromatin Immunoprecipitation (PMID 22891341), Immunohistochemistry, Western Blot, Gel Shift Assays, Immunocytochemistry/Immunfluorescence (PMID: 21270095; 21270095), and Immunoprecipitation. In Western blot a band is observed ~120 kDa. IHC applications have been mixed on mouse. For ChIP, nuclear extracts are recommended. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reference Data
Protein Families Transcription Factors
Protein Pathways mTOR signaling pathway, Pathways in cancer, Renal cell carcinoma
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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