GLP1R Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	Dot, ICC/IF, IHC, Simple Western, WB
Recommend Dilution	Dot Blot, Knockdown Validated, Immunocytochemistry/ Immunofluorescence: 1:100, Western Blot: 1:1000, Simple Western: 1:100, Immunohistochemistry-Paraffin: 1:200 - 1:400, Immunohistochemistry: 1:200
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to an internal portion of the human GLP1R protein (between residues 250-350) [UniProt P43220]
Formulation	PBS with 30% Glycerol, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	glucagon like peptide 1 receptor
Database Link	NP_002053 Entrez Gene 14652 MouseEntrez Gene 2740 Human UniProt ID P43220
Background	GLP1R (Glucagon-like peptide 1 receptor) is an incretin hormone produced in the intestine that is released in response to food intake. GLP1R plays an important role in maintaining blood glucose homeostasis and has been suggested to affect the feelings of satiety or hunger, sensation of glucose levels and control of glucagon sensitivity of islets. In addition, desensitization of GLP1R on pancreatic beta-cells is one of the causes of non insulin-dependent diabetes mellitus (NIDDM), and GLP1R is starting to play an increasingly important role in endocrine gastrointestinal tumor management.
Synonyms	MGC138331
Note	This GLP1R antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and IHC-paraffin embedded sections. In Western Blot, a band is seen ~53kDa representing GLP1R. In ICC/IF, membrane staining was observed in HeLa cells. In IHC-P, strong membrane staining was observed in mouse pancreas tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reference Data
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, GPCR, Transmembrane
Protein Pathways	Neuroactive ligand-receptor interaction

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.