St3gal3 (NM_001285521) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Siat3; Siat6; ST3GalIII; ST3N
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227256 representing NM_001285521 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGACTCTTGGTATTTGTGCGCAATCTGCTGCTAGCCCTCTGCCTCTTCCTGGTCCTGGGATTTTTGT ATTATTCTGCCTGGAAGCTACACTTACTCCAATGGGAAGACTCCAAGTATGATAGGCTGGGCTTCCTCCT GAAGCTGGACTCTAAACTGCCTGCCGAGCTAGCCACCAAGTACGCAAACTTTTCCGAGGGAGCTTGCAAA CCCGGCTACGCTTCAGCCATGATGACTGCCATCTTCCCCAGGTTCTCCAAGCCAGCACCCATGTTCCTGG ATGACTCCTTTCGCAAGTGGGCTAGGATCCGGGAGTTTGTGCCACCTTTTGGGATCAAAGGTCAAGACAA TCTGATCAAAGCCATCTTGTCAGTCACCAAAGAATACCGCCTGACCCCTGCCTTGGACAGCCTCCACTGC CGCCGCTGCATCATTGTAGGCAATGGAGGGGTCCTCGCCAACAAGTCTCTGGGGTCACGGATTGACGACT ATGACATTGTGATCAGACTGAACTCAGCACCTGTGAAGGGCTTTGAGAGGGACGTGGGCAGCAAGACCAC CCTACGCATCACCTACCCTGAGGGCGCCATGCAGCGACCTGAGCAATATGAACGAGACTCTCTCTTTGTC CTCGCTGGCTTCAAGTGGCAGGACTTCAAGTGGCTGAAGTACATCGTCTACAAGGAGAGAGTGAGTGCAT CTGATGGCTTCTGGAAGTCCGTGGCCACCCGAGTGCCCAAGGAGCCCCCTGAGATCCGCATCCTCAACCC ATACTTCATCCAGGAGGCTGCCTTCACTCTCATTGGACTGCCCTTCAACAACGGCCTCATGGGCAGAGGG AACATCCCAACCCTTGGCAGTGTGGCAGTGACCATGGCACTACACGGCTGTGATGAAGTGGCAGTCGCGG GCTTTGGCTATGACATGAACACACCCAATGCACCCCTGCACTACTATGAAACTGTGCGCATGGCAGCCAT CAAAGAGTCCTGGACACACAACATCCAGCGAGAGAAAGAGTTTCTGCGGAAGCTAGTGAAGGCACGTGTC ATCACTGACTTAAGCAGCGGTATCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001285521
Insert Size	1077 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001285521.1, NP_001272450.1
RefSeq Size	2239 bp
RefSeq ORF	1077 bp
Locus ID	20441
Gene Summary	Catalyzes the formation of the NeuAc-alpha-2,3-Gal-beta-1,4-GlcNAc-, NeuAc-alpha-2,3-Gal-beta-1,3-GlcNAc- and NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc- sequences found in terminal carbohydrate groups of glycoproteins and glycolipids. The highest activity is toward Gal-beta-1,3-GlcNAc and the lowest toward Gal-beta-1,3-GalNAc.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) differs in the 5' UTR and lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. The encoded isoform (b) is shorter than isoform a.

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