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Hck (NM_010407) Mouse Tagged ORF Clone
CAT＃: MG208418
- TrueORF®
Hck (tGFP-tagged) - Mouse hemopoietic cell kinase (Hck)
"NM_010407" in other vectors (5)
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CNY 5,800.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI849071; Bmk |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208418 representing NM_010407
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC CTGGGGGGTCGGTCTAGCTGCGAGGATCCGGGCTGCCCACGAAGCGAAGGGCGGGCGCCCAGGATGGGAT GCGTGAAGTCCAGGTTCCTCCGAGATGGAAGCAAGGCCTCAAAAACAGAGCCAAGTGCCAATCAGAAGGG CCCTGTGTATGTGCCGGATCCCACGTCCTCCAGCAAGCTGGGACCAAACAACAGCAACAGCATGCCCCCA GGGTTTGTGGAGGGCTCTGAGGATACCATTGTGGTCGCACTGTACGACTATGAGGCTATTCACCGTGAAG ACCTCAGCTTCCAGAAGGGAGACCAGATGGTGGTTCTGGAGGAGGCTGGGGAGTGGTGGAAGGCACGGTC CCTGGCTACCAAGAAGGAAGGCTACATCCCAAGCAACTATGTGGCTCGAGTTAACTCTTTGGAGACAGAA GAGTGGTTCTTCAAGGGGATCAGCCGGAAGGATGCAGAGCGCCACCTCCTGGCTCCAGGCAACATGCTGG GCTCCTTCATGATCCGGGACAGTGAGACCACCAAAGGGAGCTACTCGTTGTCTGTTCGAGACTTTGACCC CCAGCACGGAGACACCGTGAAGCACTATAAGATCCGGACGCTGGACAGTGGAGGCTTCTACATCTCTCCA AGGAGCACCTTCAGCAGCCTGCAGGAACTCGTGCTCCACTACAAGAAGGGGAAGGATGGGCTCTGCCAGA AGCTGTCAGTGCCCTGTGTGTCTCCCAAACCCCAGAAGCCATGGGAGAAAGATGCTTGGGAGATTCCTCG AGAATCCCTCCAGATGGAGAAGAAACTTGGAGCTGGGCAGTTTGGAGAAGTGTGGATGGCCACCTACAAC AAGCACACCAAAGTGGCGGTGAAGACAATGAAGCCAGGGAGCATGTCCGTGGAGGCCTTCCTGGCTGAGG CCAACCTGATGAAGTCGCTGCAGCATGACAAACTGGTGAAGCTACACGCTGTGGTCTCTCAGGAGCCCAT CTTTATTGTCACGGAGTTCATGGCCAAAGGAAGCCTGCTGGACTTTCTCAAGAGTGAAGAAGGCAGCAAG CAGCCACTGCCAAAACTCATTGACTTCTCAGCCCAGATCTCAGAAGGCATGGCCTTCATTGAGCAGAGGA ACTACATCCACCGAGACCTGAGGGCTGCCAACATCTTAGTCTCTGCATCACTGGTGTGTAAGATTGCTGA CTTTGGACTGGCACGAATCATCGAGGACAATGAGTACACAGCTCGGGAAGGAGCCAAGTTCCCCATCAAG TGGACAGCTCCTGAAGCCATCAACTTTGGTTCCTTCACCATCAAGTCAGATGTCTGGTCCTTTGGTATCC TGCTGATGGAAATTGTCACCTATGGCCGGATCCCTTACCCAGGTATGTCAAACCCAGAGGTGATTCGGGC ACTAGAGCATGGGTACCGTATGCCTCGACCAGATAACTGTCCAGAAGAGCTCTACAATATCATGATCCGC TGCTGGAAGAACCGCCCCGAGGAACGGCCCACCTTTGAATACATCCAGAGTGTGCTGGATGACTTCTACA CGGCCACTGAGAGCCAGTATCAGCAGCAGCCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208418 representing NM_010407
Red=Cloning site Green=Tags(s) LGGRSSCEDPGCPRSEGRAPRMGCVKSRFLRDGSKASKTEPSANQKGPVYVPDPTSSSKLGPNNSNSMPP GFVEGSEDTIVVALYDYEAIHREDLSFQKGDQMVVLEEAGEWWKARSLATKKEGYIPSNYVARVNSLETE EWFFKGISRKDAERHLLAPGNMLGSFMIRDSETTKGSYSLSVRDFDPQHGDTVKHYKIRTLDSGGFYISP RSTFSSLQELVLHYKKGKDGLCQKLSVPCVSPKPQKPWEKDAWEIPRESLQMEKKLGAGQFGEVWMATYN KHTKVAVKTMKPGSMSVEAFLAEANLMKSLQHDKLVKLHAVVSQEPIFIVTEFMAKGSLLDFLKSEEGSK QPLPKLIDFSAQISEGMAFIEQRNYIHRDLRAANILVSASLVCKIADFGLARIIEDNEYTAREGAKFPIK WTAPEAINFGSFTIKSDVWSFGILLMEIVTYGRIPYPGMSNPEVIRALEHGYRMPRPDNCPEELYNIMIR CWKNRPEERPTFEYIQSVLDDFYTATESQYQQQP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010407 |
| ORF Size | 1572 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010407.4, NP_034537.2 |
| RefSeq Size | 2068 bp |
| RefSeq ORF | 1575 bp |
| Locus ID | 15162 |
| UniProt ID | P08103 |
| Gene Summary | The protein encoded by this gene is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may play a role in the innate immune response and the STAT5 signaling pathway. Alternative translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms, that have different subcellular localization. [provided by RefSeq, Feb 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200941 | Hck (untagged) - Mouse hemopoietic cell kinase (Hck), transcript variant 1, (10ug) |
CNY 2,000.00 |
|
| MG227049 | Hck (tGFP-tagged) - Mouse hemopoietic cell kinase (Hck) transcript variant 1, (10ug) |
CNY 5,040.00 |
|
| MR227049 | Hck (Myc-DDK-tagged) - Mouse hemopoietic cell kinase (Hck), transcript variant 1 |
CNY 4,136.00 |
|
| MR227049L3 | Lenti ORF clone of Hck (Myc-DDK-tagged) - Mouse hemopoietic cell kinase (Hck), transcript variant 1 |
CNY 6,460.00 |
|
| MR227049L4 | Lenti ORF clone of Hck (mGFP-tagged) - Mouse hemopoietic cell kinase (Hck), transcript variant 1 |
CNY 6,460.00 |
