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Col3a1 (NM_009930) Mouse Tagged ORF Clone
CAT＃: MG211998
- TrueORF®
Col3a1 (tGFP-tagged) - Mouse procollagen, type III, alpha 1 (Col3a1)
ORF Plasmid: DDK
"NM_009930" in other vectors (2)
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CNY 16,060.00
货期*
5周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AW550625; Col3; Col3a-1; Tsk; Tsk-; Tsk-2; Tsk2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211998 representing NM_009930
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGAGCTTTGTGCAAAGTGGAACCTGGTTTCTTCTCACCCTTCTTCATCCCACTCTTATTTTGGCAC AGCAGTCCAACGTAGATGAATTGGGATGCAGCCACCTTGGTCAGTCCTATGAGTCTAGAGATGTCTGGAA GCCAGAACCATGTCAAATATGTGTCTGTGACTCAGGATCTGTCCTTTGCGATGACATAATCTGTGATGAG GAGCCACTAGACTGCCCCAACCCAGAGATCCCATTTGGAGAATGTTGTGCAATATGCCCACAGCCTTCTA CACCTGCTCCTGTGCTTCCTGATGGCCATGGACCTCAAGGCCCCAAGGGAGATCCGGGTCCTCCTGGCAT TCCTGGGAGAAATGGTGACCCTGGCCTTCCAGGACAACCAGGTCTCCCTGGGCCTCCTGGCTCCCCTGGA ATCTGTGAATCATGTCCAACTGGTGGCCAAAATTATTCTCCCCAATTCGACTCATATGATGTCAAGTCTG GAGTGGGAGGAATGGGTGGCTATCCGGGACCAGCTGGTCCACCAGGCCCTCCAGGACCCCCTGGTTCTTC TGGACATCCTGGATCTCCTGGTTCTCCTGGATACCAAGGTCCCCCTGGTGAACCTGGTCAAGCTGGTCCG GCAGGCCCTCCAGGACCTCCTGGTGCTCTTGGTCCAGCTGGTCCTGCTGGAAAGGATGGAGAGTCAGGAA GACCTGGACGACCTGGAGAGCGTGGACTGCCTGGACCTCCAGGTATCAAAGGCCCAGCTGGCATGCCTGG ATTCCCTGGTATGAAAGGACACAGAGGCTTTGATGGACGCAATGGAGAAAAGGGAGAAACAGGTGCTCCT GGACTGAAGGGTGAAAATGGTCTTCCAGGAGACAACGGAGCTCCTGGCCCCATGGGTCCTAGAGGGGCTC CTGGTGAGCGAGGACGACCAGGCCTTCCTGGAGCTGCAGGTGCTCGAGGCAATGATGGTGCTCGGGGCAG TGATGGGCAACCTGGTCCCCCTGGCCCTCCTGGAACTGCAGGATTCCCTGGATCCCCTGGTGCTAAGGGT GAAGTTGGACCTGCAGGGTCCCCTGGCTCAAATGGCTCACCAGGACAAAGAGGGGAGCCTGGACCGCAGG GACATGCTGGTGCTCAAGGGCCTCCTGGCCCTCCCGGGAATAACGGCAGTCCTGGTGGAAAGGGTGAAAT GGGTCCAGCTGGCATTCCTGGAGCCCCTGGACTAATAGGAGCAAGGGGCCCCCCTGGACCAGCAGGAACT AATGGTATACCCGGAACACGAGGTCCTTCAGGTGAACCCGGCAAGAACGGTGCGAAGGGAGAGCCAGGAG CACGCGGTGAACGGGGCGAAGCTGGTTCCCCAGGAATTCCAGGACCTAAGGGCGAAGATGGCAAAGATGG ATCACCTGGAGAACCTGGTGCAAATGGGCTTCCAGGAGCCGCAGGAGAAAGGGGTCCTAGTGGCTTCCGA GGACCTGCAGGGCCAAATGGCATCCCAGGAGAAAAGGGTCCTCCCGGAGAGCGTGGTGGCCCAGGTCCTG CAGGACCCAGAGGAGTAGCTGGAGAACCTGGCCGAGATGGAACCCCTGGAGGTCCAGGGATACGGGGTAT GCCCGGAAGTCCAGGTGGACCAGGCAATGATGGAAAACCAGGACCTCCTGGAAGTCAAGGAGAAAGTGGT CGCCCTGGTCCCCCTGGCCCATCTGGTCCCAGAGGTCAGCCTGGTGTCATGGGTTTCCCTGGTCCTAAAG GAAATGATGGTGCCCCTGGCAAGAATGGAGAACGGGGTGGCCCTGGAGGACCTGGCCTTCCAGGTCCTGC TGGAAAGAATGGGGAGACTGGACCTCAGGGTCCCCCAGGACCTACTGGCCCAGCTGGTGACAAGGGAGAT TCTGGACCCCCTGGTCCACAAGGATTACAAGGCATACCTGGTACCGGTGGTCCTCCAGGAGAAAATGGGA AACCAGGTGAACCAGGTCCAAAGGGTGAAGTCGGTGCTCCTGGAGCTCCTGGAGGCAAGGGTGATTCTGG TGCACCTGGAGAACGTGGGCCTCCTGGAACAGCAGGAATCCCTGGTGCTAGAGGTGGAGCTGGACCCCCT GGCCCTGAGGGAGGAAAGGGCCCTGCTGGTCCCCCTGGCCCTCCTGGTGCTTCTGGTTCTCCTGGTCTGC AAGGGATGCCAGGGGAAAGAGGAGGTCCTGGAAGTCCTGGCCCAAAGGGTGAAAAGGGTGAACCAGGTGG TGCAGGTGCTGACGGAGTTCCAGGAAAGGATGGGCCAAGGGGTCCTGCTGGCCCTATTGGTCCCCCTGGC CCAGCTGGTCAGCCTGGAGATAAGGGTGAAGGTGGTTCCCCTGGACTTCCAGGTATAGCTGGACCTCGAG GTGGCCCTGGTGAGAGAGGTGAACATGGCCCTCCAGGACCTGCTGGCTTCCCGGGTGCTCCTGGACAGAA TGGTGAACCAGGTGCTAAAGGAGAAAGAGGTGCCCCTGGAGAGAAAGGTGAAGGAGGCCCTCCAGGACCT GCAGGACCCACTGGCAGTTCTGGACCTGCTGGTCCGCCTGGTCCTCAGGGTGTAAAGGGTGAACGTGGTA GTCCTGGTGGTCCTGGTACTGCTGGCTTTCCCGGTGGGCGTGGTCTTCCTGGTCCTCCTGGCAATAATGG TAATCCAGGGCCCCCAGGACCCAGTGGTGCTCCTGGCAAGGACGGCCCTCCAGGTCCTGCAGGCAACAGT GGTTCTCCTGGCAACCCTGGAATAGCTGGACCAAAAGGTGATGCTGGACAGCCTGGAGAGAAGGGGCCAC CTGGTGCTCAGGGTCCTCCGGGTTCCCCAGGCCCACTTGGAATTGCAGGGCTAACTGGAGCACGAGGTCT TGCTGGACCACCAGGCATGCCAGGTCCACGGGGTAGCCCTGGACCTCAGGGTATCAAGGGTGAAAGTGGA AAACCAGGAGCCAGTGGCCATAATGGGGAACGTGGTCCTCCTGGACCCCAAGGTCTTCCTGGTCAGCCTG GTACAGCTGGTGAACCTGGAAGGGATGGAAACCCTGGATCAGATGGTCAGCCAGGTCGAGATGGATCTCC TGGTGGCAAGGGTGATCGTGGTGAAAATGGTTCTCCTGGTGCCCCAGGCGCTCCTGGTCATCCAGGCCCA CCTGGCCCTGTTGGTCCATCTGGAAAGAGTGGTGACAGAGGAGAAACTGGCCCTGCTGGTCCTTCTGGTG CTCCAGGCCCTGCTGGAGCTCGAGGTGCTCCGGGTCCCCAAGGTCCACGAGGTGACAAAGGTGAAACTGG TGAACGTGGCTCTAATGGCATCAAAGGACATCGAGGATTCCCTGGCAATCCAGGTCCTCCAGGTTCTCCT GGTGCTGCTGGTCACCAGGGTGCAATTGGTAGTCCAGGACCTGCAGGTCCCAGAGGACCAGTTGGACCAC ATGGACCTCCTGGAAAAGATGGAACAAGTGGGCATCCAGGTCCTATTGGACCACCAGGTCCTAGAGGAAA CAGAGGTGAAAGAGGATCTGAGGGCTCGCCAGGCCACCCTGGACAGCCAGGACCCCCTGGACCCCCTGGT GCCCCTGGTCCCTGCTGTGGTGGTGGTGCTGCTGCCATTGCTGGAGTTGGAGGTGAAAAGTCTGGTGGCT TTTCACCCTATTATGGAGACGATCCAATGGATTTCAAGATCAACACTGAAGAGATTATGTCTTCACTCAA GTCTGTTAATGGACAAATAGAGAGTCTTATAAGCCCTGATGGTTCTCGAAAAAACCCTGCTCGGAACTGC AGAGACCTAAAATTCTGCCACCCCGAACTCAAGAGTGGAGAATACTGGGTTGATCCTAACCAAGGCTGCA AGATGGATGCTATAAAAGTATTCTGTAACATGGAAACTGGGGAAACATGCATAAATGCCAGCCCCATGAC TGTCCCACGTAAGCACTGGTGGACAGATTCTGGTGCAGAGAAGAAACATGTTTGGTTTGGAGAATCTATG AATGGTGGTTTTCAGTTCAGCTATGGCCCTCCTGATCTTCCTGAAGATGTCGTTGATGTGCAGCTGGCAT TCCTCAGACTTCTTTCCAGCCGGGCCTCCCAGAACATTACATACCACTGCAAAAACAGTATTGCCTATAT GGATCAGGCCAGTGGCAATGTAAAGAAGTCTCTGAAGCTGATGGGATCAAATGAAGGCGAATTCAAGGCT GAAGGAAACAGCAAATTCACTTACACAGTTCTAGAGGATGGCTGTACTAAACACACTGGGGAATGGAGCA AGACAGTCTTTGAATATCAAACACGCAAGGCAATGAGACTACCCATCATAGATATCGCACCCTATGACAT TGGGGGTCCTGATCAAGAATTTGGTGTGGACATTGGCCCTGTTTGCTTTTTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211998 representing NM_009930
Red=Cloning site Green=Tags(s) MMSFVQSGTWFLLTLLHPTLILAQQSNVDELGCSHLGQSYESRDVWKPEPCQICVCDSGSVLCDDIICDE EPLDCPNPEIPFGECCAICPQPSTPAPVLPDGHGPQGPKGDPGPPGIPGRNGDPGLPGQPGLPGPPGSPG ICESCPTGGQNYSPQFDSYDVKSGVGGMGGYPGPAGPPGPPGPPGSSGHPGSPGSPGYQGPPGEPGQAGP AGPPGPPGALGPAGPAGKDGESGRPGRPGERGLPGPPGIKGPAGMPGFPGMKGHRGFDGRNGEKGETGAP GLKGENGLPGDNGAPGPMGPRGAPGERGRPGLPGAAGARGNDGARGSDGQPGPPGPPGTAGFPGSPGAKG EVGPAGSPGSNGSPGQRGEPGPQGHAGAQGPPGPPGNNGSPGGKGEMGPAGIPGAPGLIGARGPPGPAGT NGIPGTRGPSGEPGKNGAKGEPGARGERGEAGSPGIPGPKGEDGKDGSPGEPGANGLPGAAGERGPSGFR GPAGPNGIPGEKGPPGERGGPGPAGPRGVAGEPGRDGTPGGPGIRGMPGSPGGPGNDGKPGPPGSQGESG RPGPPGPSGPRGQPGVMGFPGPKGNDGAPGKNGERGGPGGPGLPGPAGKNGETGPQGPPGPTGPAGDKGD SGPPGPQGLQGIPGTGGPPGENGKPGEPGPKGEVGAPGAPGGKGDSGAPGERGPPGTAGIPGARGGAGPP GPEGGKGPAGPPGPPGASGSPGLQGMPGERGGPGSPGPKGEKGEPGGAGADGVPGKDGPRGPAGPIGPPG PAGQPGDKGEGGSPGLPGIAGPRGGPGERGEHGPPGPAGFPGAPGQNGEPGAKGERGAPGEKGEGGPPGP AGPTGSSGPAGPPGPQGVKGERGSPGGPGTAGFPGGRGLPGPPGNNGNPGPPGPSGAPGKDGPPGPAGNS GSPGNPGIAGPKGDAGQPGEKGPPGAQGPPGSPGPLGIAGLTGARGLAGPPGMPGPRGSPGPQGIKGESG KPGASGHNGERGPPGPQGLPGQPGTAGEPGRDGNPGSDGQPGRDGSPGGKGDRGENGSPGAPGAPGHPGP PGPVGPSGKSGDRGETGPAGPSGAPGPAGARGAPGPQGPRGDKGETGERGSNGIKGHRGFPGNPGPPGSP GAAGHQGAIGSPGPAGPRGPVGPHGPPGKDGTSGHPGPIGPPGPRGNRGERGSEGSPGHPGQPGPPGPPG APGPCCGGGAAAIAGVGGEKSGGFSPYYGDDPMDFKINTEEIMSSLKSVNGQIESLISPDGSRKNPARNC RDLKFCHPELKSGEYWVDPNQGCKMDAIKVFCNMETGETCINASPMTVPRKHWWTDSGAEKKHVWFGESM NGGFQFSYGPPDLPEDVVDVQLAFLRLLSSRASQNITYHCKNSIAYMDQASGNVKKSLKLMGSNEGEFKA EGNSKFTYTVLEDGCTKHTGEWSKTVFEYQTRKAMRLPIIDIAPYDIGGPDQEFGVDIGPVCFL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009930 |
| ORF Size | 4392 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009930.2, NP_034060.2 |
| RefSeq Size | 5564 bp |
| RefSeq ORF | 4395 bp |
| Locus ID | 12825 |
| UniProt ID | P08121 |
| Gene Summary | This gene encodes the alpha-1 subunit of the fibril-forming type III collagen found in bone, cartilage, dentin, tendon, bone marrow stroma and other connective tissue. The encoded protein forms homotrimeric type III procollagen that undergoes proteolytic processing during fibril formation. A majority of mice lacking the encoded protein die within two days of birth but about 5% of the animals survive to adulthood. The surviving mice exhibit severe cortical malformation and experience significantly shorter lifespan. The mutant mouse named "tight skin 2" exhibiting systemic sclerosis phenotype was found to harbor a missense point mutation in this gene. A pseudogene of this gene has been defined on chromosome 8. [provided by RefSeq, Nov 2015] |
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