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Myh9 (NM_022410) Mouse Tagged ORF Clone
CAT＃: MG224136
- TrueORF®
Myh9 (tGFP-tagged) - Mouse myosin heavy polypeptide 9 non-muscle (Myh9) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_022410" in other vectors (2)
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CNY 23,660.00
货期*
3周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Fltn; Myhn-1; Myhn1; NMHCIIA; NMMHC-A; NMMHC-IIA; TU72.6 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG224136 representing NM_022410
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCAGCAGGCTGCAGACAAGTACCTCTATGTGGATAAAAACTTCATCAATAACCCGCTGGCCCAAG CTGACTGGGCTGCCAAGAAGTTGGTATGGGTGCCTTCCAGCAAGAATGGCTTTGAACCAGCTAGCCTCAA GGAGGAGGTGGGAGAAGAGGCCATTGTAGAGCTGGTAGAGAATGGGAAGAAGGTGAAGGTGAACAAGGAC GACATCCAGAAGATGAACCCACCCAAGTTCTCCAAGGTGGAGGACATGGCAGAGCTCACGTGCCTCAACG AAGCTTCGGTGCTGCACAACCTCAAGGAGCGATACTACTCAGGGCTTATCTACACCTATTCAGGCCTGTT CTGTGTGGTCATCAACCCTTATAAGAACCTGCCCATCTACTCAGAGGAGATCGTGGAGATGTACAAGGGC AAGAAGAGGCACGAGATGCCACCCCACATCTACGCCATCACAGATACTGCCTACCGGAGCATGATGCAGG ACCGGGAAGATCAGTCCATCCTGTGCACGGGGGAGTCTGGAGCAGGGAAGACAGAGAACACCAAGAAAGT CATCCAGTACCTGGCACATGTGGCCTCCTCACACAAGAGCAAGAAGGACCAGGGGGAGTTGGAGCGGCAG CTGCTACAGGCCAACCCTATCCTAGAGGCCTTTGGAAACGCCAAGACGGTGAAGAATGACAACTCCTCTC GATTCGGTAAATTCATTCGTATCAACTTTGATGTCAATGGCTACATTGTTGGTGCCAACATTGAGACTTA TCTTCTGGAGAAATCTCGTGCTATCCGCCAAGCCAAAGAGGAGCGGACCTTCCACATCTTCTACTACCTG CTGTCTGGGGCCGGAGAACACCTGAAGACTGATCTCCTGTTGGAGCCATACAACAAATACCGCTTCCTGT CCAACGGGCACGTCACCATCCCTGGGCAGCAGGACAAGGACATGTTCCAGGAGACAATGGAGGCCATGAG AATTATGGGCATCCCAGAGGATGAGCAGATGGGCTTGCTGCGGGTCATCTCTGGGGTCCTTCAGCTTGGC AACATTGCCTTCAAGAAGGAGCGGAACACTGACCAGGCGTCCATGCCGGACAACACAGCTGCTCAAAAGG TGTCCCACCTCCTGGGGATCAATGTGACCGACTTCACCAGAGGCATCCTCACCCCACGCATCAAGGTGGG CAGAGACTATGTGCAGAAGGCGCAGACTAAAGAGCAGGCTGACTTTGCCATTGAGGCCTTGGCCAAGGCT ACCTATGAGCGGATGTTCCGCTGGCTGGTGCTTCGCATCAACAAAGCTCTGGACAAGACCAAGAGGCAGG GCGCCTCATTTATCGGGATCCTGGACATCGCTGGCTTTGAGATCTTTGATCTGAACTCCTTCGAGCAGCT GTGCATCAACTACACCAACGAGAAGCTGCAGCAGCTGTTCAACCACACCATGTTCATCCTGGAGCAGGAG GAGTACCAGCGAGAGGGCATCGAGTGGAACTTCATCGACTTCGGCCTGGACCTGCAGCCCTGCATCGACC TCATTGAGAAGCCGGCGGGTCCCCCAGGCATCCTGGCCCTGCTAGATGAGGAGTGCTGGTTTCCTAAGGC CACTGACAAGAGCTTCGTGGAGAAGGTGGTGCAGGAGCAGGGCACCCACCCCAAGTTCCAGAAGCCCAAG CAACTGAAGGACAAGGCTGATTTCTGCATTATCCACTATGCCGGCAAGGTGGACTATAAAGCTGACGAGT GGCTGATGAAGAACATGGACCCCTTGAACGACAACATCGCCACGCTGCTTCACCAGTCCTCAGACAAGTT TGTCTCTGAGCTGTGGAAGGATGTGGATCGGATCATTGGCTTGGACCAAGTGGCTGGAATGTCCGAGACA GCACTACCTGGTGCCTTCAAGACCCGGAAGGGCATGTTCCGTACTGTCGGACAGCTGTACAAGGAGCAGC TGGCCAAGCTCATGGCCACGTTGAGGAATACCAACCCCAACTTCGTGCGCTGCATCATTCCCAACCATGA GAAGAAGGCCGGCAAACTGGACCCGCACTTGGTGCTGGACCAGCTGCGCTGCAATGGCGTCCTTGAGGGC ATCCGGATCTGCCGCCAGGGCTTTCCCAACAGGGTGGTCTTCCAGGAGTTCCGGCAGAGGTATGAGATCC TCACCCCCAACTCCATCCCGAAGGGCTTCATGGATGGCAAGCAAGCGTGTGTGCTCATGATCAAAGCCTT GGAGCTTGACAGCAACCTGTACCGCATCGGCCAGAGCAAAGTGTTCTTCCGGGCAGGAGTGCTAGCCCAC CTGGAGGAAGAGCGGGACCTGAAGATCACCGATGTCATCATTGGCTTCCAGGCCTGCTGCAGGGGCTACC TGGCCAGGAAGGCCTTTGCCAAGAGGCAGCAACAGCTGACCGCCATGAAGGTCCTACAGAGGAACTGTGC TGCGTACCTCAGGCTGCGCAACTGGCAGTGGTGGAGGCTCTTCACCAAGGTCAAGCCCCTGTTGAACTCA ATAAGACATGAGGATGAGCTGTTAGCCAAGGAGGCGGAACTGACAAAGGTTCGAGAGAAACATCTGGCTG CAGAGAACAGGCTGACAGAGATGGAGACGATGCAGTCTCAGCTCATGGCAGAGAAGCTGCAGCTTCAGGA GCAGCTGCAGGCGGAGACAGAGCTGTGTGCCGAGGCTGAGGAGCTCCGGGCCCGTCTGACAGCGAAGAAG CAGGAGCTGGAGGAGATCTGCCATGACCTGGAGGCCAGGGTGGAGGAGGAGGAGGAGCGCTGCCAGTACC TGCAGGCCGAGAAGAAGAAGATGCAGCAGAACATCCAGGAACTTGAGGAGCAGTTGGAGGAGGAGGAGAG CGCCCGGCAGAAGCTGCAGCTTGAGAAGGTGACCACCGAGGCCAAGCTGAAGAAACTGGAGGAGGACCAG ATCATCATGGAGGACCAGAACTGCAAACTGGCCAAGGAGAAGAAACTGCTGGAAGACAGAGTAGCTGAAT TCACTACCAACCTCATGGAAGAGGAGGAGAAGTCCAAGAGCCTGGCCAAGCTCAAGAACAAGCACGAGGC AATGATCACCGACCTGGAAGAGCGCCTCCGTAGGGAGGAGAAGCAGAGGCAGGAGTTGGAGAAGACCCGT CGCAAGCTGGAGGGAGACTCCACAGACCTCAGTGACCAGATTGCTGAGCTCCAGGCGCAGATAGCAGAGC TCAAGATGCAGCTGGCCAAGAAGGAGGAGGAGTTGCAGGCTGCCTTGGCCAGAGTGGAAGAAGAAGCTGC TCAGAAGAATATGGCCCTGAAGAAGATCCGAGAACTGGAAACTCAGATCTCTGAGCTCCAGGAGGACCTG GAGTCGGAGCGAGCCTCCAGGAATAAAGCCGAGAAGCAGAAACGGGATCTGGGAGAGGAGCTGGAGGCGC TGAAGACAGAGCTGGAGGACACGCTGGACTCCACGGCTGCCCAGCAGGAGCTGAGGTCGAAGCGTGAGCA GGAGGTGAGCATCCTGAAGAAGACTCTGGAGGACGAGGCCAAGACCCATGAGGCCCAGATCCAGGAGATG AGGCAGAAGCACTCACAGGCTGTGGAGGAGCTGGCAGATCAGTTGGAGCAGACGAAGCGGGTAAAAGCTA CCCTTGAGAAGGCGAAGCAGACCCTGGAGAATGAGCGGGGAGAGCTGGCCAATGAGGTGAAGGCCCTGCT GCAAGGCAAGGGCGACTCAGAGCACAAGCGCAAGAAGGTGGAGGCGCAGCTGCAAGAACTGCAGGTCAAG TTCAGCGAGGGAGAGCGCGTGCGAACCGAACTGGCCGACAAGGTCACCAAGCTGCAGGTTGAACTGGACA GTGTGACCGGTCTCCTTAGCCAGTCTGACAGCAAGTCCAGCAAGCTTACGAAGGACTTCTCTGCGCTGGA GTCCCAGCTTCAGGACACACAGGAGTTGCTCCAGGAGGAGAACCGGCAGAAGCTGAGCCTGAGCACCAAG CTCAAGCAGATGGAGGATGAGAAAAACTCCTTCAGGGAGCAGCTGGAGGAGGAGGAGGAGGCCAAGCGCA ACTTGGAGAAGCAGATCGCCACGCTCCATGCCCAGGTGACCGACATGAAGAAGAAGATGGAGGACGGTGT AGGGTGCCTGGAGACTGCAGAGGAGGCGAAGCGGAGGCTTCAGAAGGACTTGGAAGGCCTGAGCCAGCGG CTTGAGGAGAAGGTGGCTGCCTACGATAAGCTGGAGAAGACCAAGACACGGCTGCAGCAGGAGCTGGACG ACCTGCTGGTTGACCTGGACCACCAGCGGCAGAGCGTCTCCAACCTGGAAAAGAAGCAGAAGAAGTTCGA CCAGCTCCTAGCCGAGGAGAAGACCATCTCGGCCAAGTATGCAGAGGAGCGTGACCGAGCTGAGGCTGAG GCCCGTGAGAAGGAGACAAAGGCGCTATCACTGGCCCGGGCGCTTGAGGAGGCCATGGAGCAGAAGGCAG AGCTGGAGCGGCTCAACAAGCAGTTCCGCACGGAGATGGAGGACCTCATGAGCTCCAAGGATGACGTGGG CAAGAGTGTCCACGAGCTGGAGAAGTCCAAGCGGGCCTTGGAGCAGCAGGTGGAGGAGATGAAGACCCAG CTGGAGGAGCTGGAGGATGAGCTGCAGGCCACGGAGGATGCCAAGCTCCGCCTGGAGGTGAACCTGCAGG CCATGAAGGCCCAGTTTGAGCGGGATCTGCAGGGCCGGGATGAACAGAGCGAGGAGAAGAAGAAGCAGCT GGTCAGACAGGTGCGGGAGATGGAGGCGGAGCTGGAGGATGAGAGGAAGCAGCGCTCCATGGCCATGGCC GCACGCAAGAAACTGGAGATGGATCTGAAGGACCTGGAGGCACACATTGACACAGCCAATAAGAACCGGG AAGAGGCCATCAAACAGCTGCGGAAGCTTCAGGCCCAGATGAAGGACTGCATGCGGGAGCTGGACGACAC GCGCGCCTCCCGGGAGGAGATCCTGGCGCAGGCCAAGGAGAATGAGAAGAAGCTGAAGAGCATGGAGGCC GAGATGATTCAGCTGCAGGAGGAACTGGCAGCTGCTGAGCGTGCTAAGCGTCAGGCCCAACAGGAACGGG ACGAGCTGGCTGATGAGATCGCCAACAGCAGTGGCAAAGGGGCCCTAGCATTAGAGGAGAAGCGGCGACT GGAGGCCCGCATTGCCCAGCTGGAGGAGGAGCTGGAGGAGGAACAGGGCAACACGGAGCTGATCAACGAT CGGCTGAAGAAGGCCAACCTGCAGATCGACCAAATAAACACCGACCTGAACCTGGAACGCAGCCACGCAC AGAAGAATGAGAATGCGCGACAGCAGCTGGAACGCCAGAACAAGGAGCTCAAGGCCAAGCTGCAGGAAAT GGAGAGTGCTGTCAAGTCCAAATACAAGGCCTCCATCGCGGCCTTGGAGGCCAAAATTGCACAGCTGGAG GAACAGCTGGACAACGAGACCAAGGAGCGCCAGGCAGCCTCCAAGCAGGTGCGCCGGACGGAGAAGAAGC TGAAGGACGTGCTGCTGCAGGTGGAGGACGAGCGGAGGAACGCGGAACAGTTCAAGGACCAGGCTGACAA GGCGTCCACCCGCCTGAAGCAGCTTAAACGGCAGCTAGAGGAGGCTGAAGAGGAGGCCCAGCGGGCCAAT GCCTCACGCCGGAAGCTGCAGCGTGAGCTGGAAGATGCCACAGAGACCGCTGATGCTATGAACCGCGAGG TCAGCTCCCTGAAGAACAAACTGAGGCGTGGGGACCTGCCATTTGTCGTGACTCGCCGAATTGTTCGGAA AGGCACTGGCGACTGCTCAGACGAGGAGGTCGACGGTAAAGCAGATGGGGCCGATGCCAAGGCAGCTGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG224136 representing NM_022410
Red=Cloning site Green=Tags(s) MAQQAADKYLYVDKNFINNPLAQADWAAKKLVWVPSSKNGFEPASLKEEVGEEAIVELVENGKKVKVNKD DIQKMNPPKFSKVEDMAELTCLNEASVLHNLKERYYSGLIYTYSGLFCVVINPYKNLPIYSEEIVEMYKG KKRHEMPPHIYAITDTAYRSMMQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKSKKDQGELERQ LLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDVNGYIVGANIETYLLEKSRAIRQAKEERTFHIFYYL LSGAGEHLKTDLLLEPYNKYRFLSNGHVTIPGQQDKDMFQETMEAMRIMGIPEDEQMGLLRVISGVLQLG NIAFKKERNTDQASMPDNTAAQKVSHLLGINVTDFTRGILTPRIKVGRDYVQKAQTKEQADFAIEALAKA TYERMFRWLVLRINKALDKTKRQGASFIGILDIAGFEIFDLNSFEQLCINYTNEKLQQLFNHTMFILEQE EYQREGIEWNFIDFGLDLQPCIDLIEKPAGPPGILALLDEECWFPKATDKSFVEKVVQEQGTHPKFQKPK QLKDKADFCIIHYAGKVDYKADEWLMKNMDPLNDNIATLLHQSSDKFVSELWKDVDRIIGLDQVAGMSET ALPGAFKTRKGMFRTVGQLYKEQLAKLMATLRNTNPNFVRCIIPNHEKKAGKLDPHLVLDQLRCNGVLEG IRICRQGFPNRVVFQEFRQRYEILTPNSIPKGFMDGKQACVLMIKALELDSNLYRIGQSKVFFRAGVLAH LEEERDLKITDVIIGFQACCRGYLARKAFAKRQQQLTAMKVLQRNCAAYLRLRNWQWWRLFTKVKPLLNS IRHEDELLAKEAELTKVREKHLAAENRLTEMETMQSQLMAEKLQLQEQLQAETELCAEAEELRARLTAKK QELEEICHDLEARVEEEEERCQYLQAEKKKMQQNIQELEEQLEEEESARQKLQLEKVTTEAKLKKLEEDQ IIMEDQNCKLAKEKKLLEDRVAEFTTNLMEEEEKSKSLAKLKNKHEAMITDLEERLRREEKQRQELEKTR RKLEGDSTDLSDQIAELQAQIAELKMQLAKKEEELQAALARVEEEAAQKNMALKKIRELETQISELQEDL ESERASRNKAEKQKRDLGEELEALKTELEDTLDSTAAQQELRSKREQEVSILKKTLEDEAKTHEAQIQEM RQKHSQAVEELADQLEQTKRVKATLEKAKQTLENERGELANEVKALLQGKGDSEHKRKKVEAQLQELQVK FSEGERVRTELADKVTKLQVELDSVTGLLSQSDSKSSKLTKDFSALESQLQDTQELLQEENRQKLSLSTK LKQMEDEKNSFREQLEEEEEAKRNLEKQIATLHAQVTDMKKKMEDGVGCLETAEEAKRRLQKDLEGLSQR LEEKVAAYDKLEKTKTRLQQELDDLLVDLDHQRQSVSNLEKKQKKFDQLLAEEKTISAKYAEERDRAEAE AREKETKALSLARALEEAMEQKAELERLNKQFRTEMEDLMSSKDDVGKSVHELEKSKRALEQQVEEMKTQ LEELEDELQATEDAKLRLEVNLQAMKAQFERDLQGRDEQSEEKKKQLVRQVREMEAELEDERKQRSMAMA ARKKLEMDLKDLEAHIDTANKNREEAIKQLRKLQAQMKDCMRELDDTRASREEILAQAKENEKKLKSMEA EMIQLQEELAAAERAKRQAQQERDELADEIANSSGKGALALEEKRRLEARIAQLEEELEEEQGNTELIND RLKKANLQIDQINTDLNLERSHAQKNENARQQLERQNKELKAKLQEMESAVKSKYKASIAALEAKIAQLE EQLDNETKERQAASKQVRRTEKKLKDVLLQVEDERRNAEQFKDQADKASTRLKQLKRQLEEAEEEAQRAN ASRRKLQRELEDATETADAMNREVSSLKNKLRRGDLPFVVTRRIVRKGTGDCSDEEVDGKADGADAKAAE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_022410 |
| ORF Size | 5880 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_022410.3, NP_071855.2 |
| RefSeq Size | 7371 bp |
| RefSeq ORF | 5883 bp |
| Locus ID | 17886 |
| UniProt ID | Q8VDD5 |
| Gene Summary | During cell spreading, plays an important role in cytoskeleton reorganization, focal contacts formation (in the margins but not the central part of spreading cells), and lamellipodial retraction; this function is mechanically antagonized by MYH10 (By similarity). Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping.[UniProtKB/Swiss-Prot Function] |
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