Irx5 (NM_018826) Mouse Tagged ORF Clone

CAT#: MR207753

  • TrueORF®

Irx5 (Myc-DDK-tagged) - Mouse Iroquois related homeobox 5 (Drosophila) (Irx5)

ORF Plasmid: DDK tGFP



  "NM_018826" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 5,488.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR207753 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCTACCCGCAGGGCTACTTGTACCAGCCGTCCGCCTCGCTGGCGCTCTACTCGTGCCCCGCATACA
GCACCAGCGTCATTTCGGGGCCCCGCACGGATGAGCTCGGCCGCTCTTCTTCGGGCTCCGCGTTCTCGCC
CTACGCTGGCTCCACTGCCTTCACGGCTCCCTCGCCCGGCTACAACTCGCACCTCCAGTACGGCGCCGAC
CCCGCGGCTGCGGCCGCCGCTGCCTTCTCTTACGTGGGCTCTCCCTACGACCACACTCCCGGTATGGCAG
GTTCCTTGGGATATCACCCTTATGCAGCACCTCTGGGCTCCTATCCTTATGGGGACCCCGCGTACCGGAA
GAATGCCACTCGGGACGCTACAGCCACTCTCAAGGCCTGGCTCAACGAGCACCGCAAGAACCCGTACCCC
ACCAAGGGAGAGAAGATCATGCTGGCCATTATCACCAAGATGACCCTCACCCAGGTGTCCACCTGGTTTG
CCAACGCGCGCCGGCGCCTCAAGAAAGAGAACAAGATGACGTGGACGCCACGGAACCGCAGCGAGGACGA
GGAGGAGGAGGAGAACATCGATCTGGAGAAGAACGACGAGGACGAGCCACAGAAGCCCGAGGACAAGGGC
GACCTCGAGGGCCCCGAATCAGGAGGAGCAGAGCAGAAGGCGACTGCGGGCTGCGAACGGCTGCAGGGGC
CGCTCAGCCCCGCCGGCAAGGAGACCGAAGGCAGCCTCAGCGACTCGGATTTTAAGGAGTCGTCCTCCGA
AGGCCGCCACGATGAGCTGCCCAGGCCCCCGCGCGCGGGCGAGTCCTCCCCGGCTGGGCCCGCAACCGCT
CGTCTGGCGGAAGACGCGGGTCCTCACTACCCCGCGAGTGTGCCGGCTCCCGGCCCGCACCCATCAGCCG
GAGAGCTGCCCCCCGGCTCGGGCGGATCCTCGGTCATCCACTCGCCACCGCCACCTCCGCCACCGCCTCC
GGCCGTGCTAGCCAAGCCCAAACTGTGGTCTCTGGCCGAGATCGCCACATCCTCGGACAAGGTCAAGGAC
GGGGGCGGAGGGAGCGAGGGCTCTCCATGCCCACCGTGCCCAGGGCCCATGGGCGGGCAAACGCTTGGAG
GCAGCCGCGCGTCTCCTGCCCCAGCGCCCGCACGCTCACCCTCCGCGCAGTGTCCCTTTCCGGGCGGGAC
GGTGCTGTCCCGGCCTCTTTATTACACGGCGCCCTTCTATCCGGGCTACACGAACTATGGCTCCTTCGGA
CATCTTCACGGCCACCCAGGGCCAGGGCCAAGCCCTACAGCGGGTCCTGGCTCTCATTTCAATGGATTAA
ACCAGACGGTGTTGAATCGTGCGGACGTTTTGGCTAAAGACCCGAAAATGTTGCGGAGCCAGTCTCAGCT
AGACCTGTGCAAAGACTCCCCCTATGAGTTGAAGAAAGGTATGTCCGACATT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR207753 protein sequence
Red=Cloning site Green=Tags(s)

MSYPQGYLYQPSASLALYSCPAYSTSVISGPRTDELGRSSSGSAFSPYAGSTAFTAPSPGYNSHLQYGAD
PAAAAAAAFSYVGSPYDHTPGMAGSLGYHPYAAPLGSYPYGDPAYRKNATRDATATLKAWLNEHRKNPYP
TKGEKIMLAIITKMTLTQVSTWFANARRRLKKENKMTWTPRNRSEDEEEEENIDLEKNDEDEPQKPEDKG
DLEGPESGGAEQKATAGCERLQGPLSPAGKETEGSLSDSDFKESSSEGRHDELPRPPRAGESSPAGPATA
RLAEDAGPHYPASVPAPGPHPSAGELPPGSGGSSVIHSPPPPPPPPPAVLAKPKLWSLAEIATSSDKVKD
GGGGSEGSPCPPCPGPMGGQTLGGSRASPAPAPARSPSAQCPFPGGTVLSRPLYYTAPFYPGYTNYGSFG
HLHGHPGPGPSPTAGPGSHFNGLNQTVLNRADVLAKDPKMLRSQSQLDLCKDSPYELKKGMSDI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_018826
ORF Size 1455 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_018826.2, NP_061296.1
RefSeq Size 2421 bp
RefSeq ORF 1455 bp
Locus ID 54352
UniProt ID Q9JKQ4
MW 50.8 kDa
Gene Summary Establishes the cardiac repolarization gradient by its repressive actions on the KCND2 potassium-channel gene. Required for retinal cone bipolar cell differentiation. May regulate contrast adaptation in the retina and control specific aspects of visual function in circuits of the mammalian retina. Involved in craniofacial and gonadal development (By similarity). Modulates the migration of progenitor cell populations in branchial arches and gonads by repressing CXCL12.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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