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Mbd1 (NM_013594) Mouse Tagged ORF Clone
CAT＃: MR209180
- TrueORF®
Mbd1 (Myc-DDK-tagged) - Mouse methyl-CpG binding domain protein 1 (Mbd1)
"NM_013594" in other vectors (3)
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CNY 4,376.00
CNY 5,230.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Cxxc3; PCM1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209180 representing NM_013594
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGAGTCCTGGCAGGACTGCCCAGCCCTGGGCCCTGGCTGGAAACGCCGAGAGTCCTTTCGAAAGT CAGGGGCCTCGTTTGGACGCTCAGACATCTATTACCAGAGCCCCACAGGAGAAAAGATCCGAAGCAAAGT TGAGCTGACTCGGTACTTGGGCCCTGCATGTGATCTTACCCTGTTTGACTTCAGACAAGGCACCTTATGC CATCCCATTCCCAAGACTCATCCCTTGGCTGTCCCCAGCAAGAAGAAAAAGAAGCCTTCTAAACCAGCCA AGACTAAGAAACAGCAGGTTGGGCTGCAGCGAAGTGAGGTCAGGAGAGAGACTCCACAGGGGGAATATAA GGCCCCTACTGCCACAGCTCTAGCTTCACTATCTGTGTCTGCATCTGCGTCTTCGTCTGCATCTGCATCT GCGTCTTCGTCTGCATCTGCATCTGCATCTGCGTCTTCACATGCACCTGTGTGCTGTGAGAATTGTGGAA TCCACTTTTCATGGGATGGTGTCAAAAGGCAGAGACTTAAGACGTTGTGCAAAGATTGTCGAGCACAGAG AATCGCCTTCAACCGCGAACAGAGAATGTTTAAGCGAGTCGGCTGTGGGGACTGTGCAGCTTGCCTGGTG AAAGAGGACTGTGGGGTGTGCTCCACCTGTCGCCTGCAACTGCCCAGTGATGTGGCCTCAGGGCTTTACT GCAAGTGTGAGCGGAGACGATGCCTGCGGATTATGGAGAAGAGCCGAGGGTGTGGCGTGTGTCGGGGTTG TCAGACCCAAGAGGACTGTGGCCATTGCTGCATCTGTCTTCGCTCTCCCCGCCCTGGTCTCAAGCGCCAG TGGAGGTGTTTGCAACGGCGCTGTTTTTGGGGTAAACGTGATTCTAGTAAGAGAGGCTCGAAGGTAGCGT CTCAGCGTCACTCCCAAGCCCCGCCACTTCCTCCACATCCTGCATCACAGTACACAGAGCCCACAGAGCT GCACATCAGCGACATAGCACCCACATCACCTGCTGAGTTCATCTATTACTGTGTAGACGAGGACGAGGAC GAGCTAAAGCGGCTGCTGCCCAGTGCTGGGTCAGGGTCTGGGGAGGGAGCAGGATTGCGTCCATATCAGA CCCATCAGACCCATCAAAAGAGGCCTGCTTCTGCTCGACAACTCCAACTGAGCTCTCCCTTAAAGGCCCC TTGGGCTGTGGTCACAGCACCACCAGGCCCTGTCCGAGATTCAAGAAAGCAGCAAGCAGGTAGAGGCTCT GTACTGCCCCAGCCTGACACAGACTTTGTGTTTTTACAAGAGGGTACCAGCAGTGCCATGCAGATGCCTG GCACTGCTGCAGCTTGCACAGAAGTCCCAGTACAGGCGGCCCAGTGCTCTGCCCCGAGCTGGGTTGTGGC CTTACCCCAGGTGAAGCAAGAGACGGCAGATGCTCCAGAGGAGTGGACAGCAGTCACGACCTTCCTGACT TCCTCCACGCTGCAGTCTGGCTTCCCTAGCAAGGCTGCAGATCCAGACCTTTCACCTGTGAAACAAGAGC CCCCTGGCCCTGAAGAGGATGGAGAAGAGAAAAAAGATGATGTCTCTGAAACAACCCCAGCAGAGGAGAT AGGAGGGGTTGGCACGCCAGTGATCACGGAGATTTTCAGCCTGGGTGGAACCCGCCTCCGAGATGCAGAA GCCTGGTTGCCAAGGCTACATAAACTGTTAGCAGTAAATGAAAAAGAGTATTTTACTGAGCTGCAGTTGA AAGAAGAAGTTTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209180 representing NM_013594
Red=Cloning site Green=Tags(s) MAESWQDCPALGPGWKRRESFRKSGASFGRSDIYYQSPTGEKIRSKVELTRYLGPACDLTLFDFRQGTLC HPIPKTHPLAVPSKKKKKPSKPAKTKKQQVGLQRSEVRRETPQGEYKAPTATALASLSVSASASSSASAS ASSSASASASASSHAPVCCENCGIHFSWDGVKRQRLKTLCKDCRAQRIAFNREQRMFKRVGCGDCAACLV KEDCGVCSTCRLQLPSDVASGLYCKCERRRCLRIMEKSRGCGVCRGCQTQEDCGHCCICLRSPRPGLKRQ WRCLQRRCFWGKRDSSKRGSKVASQRHSQAPPLPPHPASQYTEPTELHISDIAPTSPAEFIYYCVDEDED ELKRLLPSAGSGSGEGAGLRPYQTHQTHQKRPASARQLQLSSPLKAPWAVVTAPPGPVRDSRKQQAGRGS VLPQPDTDFVFLQEGTSSAMQMPGTAAACTEVPVQAAQCSAPSWVVALPQVKQETADAPEEWTAVTTFLT SSTLQSGFPSKAADPDLSPVKQEPPGPEEDGEEKKDDVSETTPAEEIGGVGTPVITEIFSLGGTRLRDAE AWLPRLHKLLAVNEKEYFTELQLKEEVL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013594 |
| ORF Size | 1764 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013594.3 |
| RefSeq Size | 2672 bp |
| RefSeq ORF | 1767 bp |
| Locus ID | 17190 |
| UniProt ID | Q9Z2E2 |
| MW | 64.6 kDa |
| Gene Summary | Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 can also repress transcription from unmethylated promoters.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219389 | Mbd1 (untagged) - Mouse methyl-CpG binding domain protein 1 (Mbd1), (10ug) |
CNY 7,510.00 |
|
| MR209180L3 | Lenti ORF clone of Mbd1 (Myc-DDK-tagged) - Mouse methyl-CpG binding domain protein 1 (Mbd1) |
CNY 7,130.00 |
|
| MR209180L4 | Lenti ORF clone of Mbd1 (mGFP-tagged) - Mouse methyl-CpG binding domain protein 1 (Mbd1) |
CNY 7,130.00 |
