Ralgapa1 (NM_001003719) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2310003F20Rik; 4930400K19Rik; AI563624; Garnl1; GRIPE; mKIAA0884; Tulip1
Vector	pCMV6-AC-Myc-DDK
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR220421 representing NM_001003719 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCTCTAAGAAGCCGCACGGGGACGTGAAGAAGTCCACCCAGAAGGTGCTGGACACCAAGAAGGACG CGCTGACCCGCCTCAAGCACCTGCGCATCGTCATCGAGAATGCAGAATCTATTGACCTTAAACAGTTTTT TGACCAACATTTTTCACATATATACTATGTGTTTTTTGAAAATTTTGTGACTATTGAAGCTAGTCTTAAA CAGAAAGGTCACAAGTCTCAAAGAGAGGAATTAGATGCTATACTTTTTATTTTTGAGAAAATTTTACAAC TTCTTCCAGAAAGAATTCATCAGCGATGGCAATTCCATAGTATTGGGTTGATTTTAAAGAAACTACTGCA CACAGGAAACTCCTTAAAGATCAGACGGGAAGGTGTTCGTCTCTTCTTGTTATGGTTGCAAGCTCTTCAG GATAACTGCAGCAAAGAACAGCTCTGGATGTTTTCATGCTTGATCCCTGGATTCTCAGCACCACAATCCG AATATGGACCTCGGACTTTAGATAATCTTATTAATCCTCCACTCAGCCTTCAAGAAACTCAAGTAACTAT AGAGGAAGTCACTCCTCTTGTTCCGCCACAGTCAGGAGATAAAGGACAAGAAGACCTCACAAGCTATTTT CTTGAAGCACTTTTAAAATACATAGTAATTCAGGTAAAAAGTTTAGAATGGAAGAACAAAGAAAACCAAG AAAGGGGATTTTCATTTTTGTTTTCACATTTTAAGAAGTTTTACTTGCCTTATATTTTCCCAAACATTTG CAAGGAGAATAGTTTGTATCATCCTGTACTTGATATCCCACAGATCAGACCAAAGCCTCATTATGTGATG ATAAAGAAGGATGCTGAAACCAATGAAACAATATATTGTACAAAAGAGCCTTTCATCCAGGCTCGGGTTA TTGTCATTCGTTGGCTGGTTTCTTTCTGGCTGGAGCCAAAACCACATTCAGGACCTAATATTCCTGGGAT GGAAGGTGAAGTCTTGCCAAAGAATATTCAGAGAGCAGCTGCTAGTTTGGTTTCCAGAGAAGAAAGCAAA AATGATACTGTTGATAAAGTAGACAAGTCTGCAGAGCCTGAGCAGTCTCATTCCAACACAAGCACTCTCA CGGAGCGAGAGCCTAGCTCTTCCAGTCTCTGCAGCATCGACGAAGAACATCTCACAGACATCGAAATTGT TCGCAGAGTGTTTTCATCTAAGAGAAGCAATGTAAACTTTGTCACAGAGATATTTCGTCAGGCATTTTTA TTACCAATTTGTGAAGCAGCAGCTATGAGAAAAGTGGTGAAAGTGTATCAAGAATGGATTCAACAAGAGG AAAAGCCTTTGTTTATGCAAGAGCCTGAGGACACTGCGATCACTTGTTCAGATATACCCTGCAGCGAAAC TGTTGCAGACCATGACAGTGCAATAGAAGATGGAGAGAAAAGAGAAGAGGAAAATGGGACCAGTACTTCT GAACATGTTCGCAATTCTAGCTGGACAAAAAATGGTTCCTACCAAGAAGCTTTTCATGTCTGTGAAGAAG CCACAGAACAGAATATTCAAGCTGGCACTCAGGCCGTTTTGCAGGTGTTTATTATAAACTCATCGAACAT ATTTCTTCTTGAACCTGCAAATGAAATAAAGAATCTTCTGGATGAGCATACAGATATGTGTAAACGCATT CTTAATATTTATCGATACATGGTTGTGCAAGTATCAATGGACAAAAAGACCTGGGAACAGATGCTGCTCG TGCTGCTCAGAGTCACGGAGTCCGTACTGAAGATGTCGTCACAAGCTTTTCTGCAGTTCCAGGGGAAAAA AAGCATGACCCTGGCGGGTCGACTTGCAGGACCACTTTTTCAGACTCTCATTGTTGCCTGGATCAAAGCA AATCTAAATGTATACATTTCTCGAGAACTTTGGGATGACTTACTTTCGGTATTGTCATCATTGACCTATT GGGAAGAATTGGCCACTGAGTGGTCACTGACCATGGAGACACTGACTAAAGTTTTGGCTAGAAATTTATA TAGTTTGGATCTCAGTGATTTGCCATTGGATAAGCTCAGTGAACAGAAACAAAAAAAGCACAAAGGGAAA GGAGTTGGACACGAATTTCAGAAAGTTTCAGTTGACAAGTCATTTTCTAGAGGATGGAGTCGCGATCAGC CTGGCCAGGCACCTATGAGACAGAGAAGTGCAACGACCACTGGCTCCCCAGGAACTGAGAAGGCCAGGAG TATAGTACGGCAAAAAACTGTTGATATTGATGATGCTCAAATCCTTCCCCGCTCAACCAGAGTCAGACAT TTTTCACAGAGTGAAGACACTGGAAATGAAGTTTTTGGTGCTTTGCATGAGGAACAGCCACTGCCTCGAA GTAGCAGCACTTCTGACATCTTGGAACCATTCACTGTTGAGCGAGCCAAAGTAAATAAAGAGGACACGAG CCCCAAACTGCCTCCTCTTAATAGTGAGACTGGTGGCAACAGCGCCAATGTTCCTGACCTGATGGATGAG TTTATCGCAGAGCGACTTCGAAGTGGCAATGCCTCGACTATGACAAGAAGGGGGAGTAGTCCAGGCAGCC TTGAAATCCCGAAAGACCTCCCTGATATTCTAAACAAGCAGAACCAGATGCGGCCTGTTGATGACCCTGG TGTACCCTCCGAATGGACGTCTCCAGCCAGTGCAGGGAGCAGTGATCTCATGAGCTCAGATAGTCACTCG GATTCCTTCAGTGCCTTCCAGTGTGAGGGACGAAAATTTGACAACTTTGGCTTCGGGACTGACATTGGGA TCCCATCTTCTGCTGATGTGGATTTAGGTTCTGGCCATCATCAAAGCACTGAGGAGCAGGAAGTGGCCAG CCTAACTACGCTTCATTTAGATTCAGAAACAAGCAGCCTTAATCAGCAAGCCTTCTCTGCAGAAGTTGCA ACCGTTACTGGTTCAGAAAGTGCTTCCCCCGTTCATTCAGCTCTGGGATCCAGGTCGCAGACCCCCTCCC CATCTACACTGAGCAGAGCTCACATAGAACAGAAGGACCTACAGCTCGATGAGAAGCTCCACCACTCTGT TCTTCAGACGCCGGATGACCTAGAAATCAGTGAATTTCCATCTGAATGTTGTAGCGTGATGGCAGGAGGT ACTCTCACTGGGTGGCATGCTGATGTTGCTACTGTTATGTGGCGCAGAATGCTGGGCATTTTGGGAGATG TCAATGCTATTATGGATCCTGAAATACATGCTCAAGTTTTTGATTACCTCTGTGAACTTTGGCAGAATCT AGCCAAGATCAGAGATAACCTTGGCATTTCAGCTGACAACCTGACTTCCCCTTCTCCACCCGTCCTGATT CCTCCACTAAGAATTCTTACACCTTGGCTTTTTAAGGCAACCATGTTGACTGATAAGTATAAACAAGGTA AATTACATGCATATAAACTTATTTGTAATACAATGAAGAGAAGACAAGATGTATCTCCAAATAGAGATTT TTTAACACATTTCTACAATATAATGCATTGTGGATTGCTTCATATTGACCAGGACATTGTCAATACGATC ATCAAGCACTGCTCACCTCAGTTTTTTTCACTCGGTTTGCCTGGGGCCACGATGCTTATTATGGATTTTA TTATAGCAGCTGGGAGAGTGGCTTCTTCGGCTTTTCTTAATGCACCAAGAGTGGAAGCACAAGTTCTTCT GGGATCTTTAGTTTGCTTTCCCAACTTATATTGTGAATTGCCTGCTCTCCATCCCAACATTCCTGATATT GCTGTGTCTCAATTTACAGATGTTAAGGAACTTATAATTAAAACTGTATTAAGCTCGGCAAGAGATGAGC CCTCTGGTCCTGCACGATGTGTAGCACTTTGCAGTTTAGGTATTTGGATTTGTGAAGAATTAGTCCACGA ATCTCATCATCCTCAAATTAAAGAAGCACTGAATGTAATTTGTGTTTCATTAAAGTTTACTAATAAAACA GTTGCCCACGTCGCCTGTAACATGCTTCACATGCTGGTTCACTATGTCCCTAGACTTCAGATTCACCAGC CGCAGTCTCCCCTGAAAATTATTCAGATCCTAATAGCAACTATCACCCACCTTTTGCCAAGTACAGAAGC TTCATCATATGAAATGGACAAGAGGTTGGTTGTCTCATTACTTCTTTGCCTTCTGGACTGGATCATGGCC TTACCTTTAAAGACACTGCTCCAACCAGTCCATGCTACAGGAGCAGAAAATGATAAGACCGAAAAGTCTG TTCTCAACTGCATTTATAAGGTATTACATGGGTGTGTCTATGGAGCGCAAAGTTTTAGCAATCCCAAGTA CTTTCCAATAAGCCTTTCTGATTTGGCATCTGTAGATTATGATCCGTTTATGCATTTGGAAAGTCTGAAA GAACCTGAACCTTTACACTCTCCAGATTCAGAGCGGTCTTCTAAACTGCAGCCTGTGACAGAAGTGAAAA CTCAGATGCAACAAGGATTAATCTCTATAGCAGCCCGTACTGTTATTACCCATCTGGTGAATCACTTGGG CCATTATCCAATGAGTGGTGGTCCTGCTATGCTAACAAGTCAGGTGTGTGAAAATCATGACAATCATTAC AGTGAAAGTACTGAACTTTCTCCTGAGCTCTTTGAAAGTCCAAACATCCAGTTCTTTGTGCTGAATAATA CAACCTTAGTGTCCTGTATCCAGATAAGATCGGAAGAGAGTATGCCTGGAGGAGGTCTAGCTGCTGGCCT TGTGTCAGCCAACTCAAATGTTAGAATCATAGTACGTGATCTCTCTGGAAAGTACTCATGGGATTCTGCC ATACTGTATGGACCACCCATTGTAAGTGGTTTGCCTGAACCTACATCTTTCATACTTTCAATGTCTGACC AAGAGAAACCGGAAGAGCCTCCTACATCTAACGAATGCTTAGAAGACATCGCAGTAAAAGATGGGCTTTC TCTCCAGCTTAGAAGATTTAGAGAAACTGTACCAACTTGGTCTACGATACGAGAGGAAGAAGATGTTCTT GACGAGCTCCTGCAGTATTTAGGCACTACAAGTCCTGAGTGTTTACAGAGAACGGGAATCTCCCTCAATG TTCCTGCTCCACAGCCTCTGTGCATTTCTGAAAAACAGGAGAATGATGTCATTAATGCTATCCTTAAGCA ATACACGGAAGAAAAAGAGTTTGTAGAGAAGCACTTTAATGACTTAAACATGAAAGCCTCAGAACAAGAT GAGCCAACACCTCAGAAACCTCAGTCAGCTTTTTATTACTGCAGATTGCTTCTTAGTATATTGGGAATGA ATTCCTGGGACAAACGGAGGAGCTTTCATCTCTTGAAGAAAAATGAAAAGCTGCTCAGAGAACTTAGGAA CTTGGATTCAAGGCAATGCCGAGAGACACACAAGATTGCAGTATTTTACGTTGCTGAAGGACAAGAAGAT AAATACTCCATTCTCACCAATATAGGAGGAAGCCAAGCATATGAAGATTTTGTAGCTGGCCTTGGTTGGG AGGTAAACCTTACAAACCACTGTGGCTTCATGGGAGGCCTTCAGAAGAACAGAAGCACTGGACTGACCAC TCCCTATTTTGCTACCTCGACAGTAGAAGTAATATTTCATGTATCTACACGAATGCCTTCTGATTCTGAT GACTCTCTGACCAAAAAATTGAGACATCTAGGAAATGATGAAGTGCACATTGTTTGGTCAGAACATACTA GAGACTACAGGAGAGGAATTATTCCTACTGAGTTTGGTGATGTCCTCATTGTAATATACCCAATGAAAAA CCACATGTTCAGTATCCAGATCATGAAAAAACCAGAGGTTCCCTTCTTTGGTCCACTTTTTGATGGTGCT ATTGTGAATGGAAAAGTTCTGCCCATTATGGTTCGATCCACAGCAATAAATGCTAGCCGTGCTCTGAAAT CACTGATTCCATTGTATCAAAATTTCTATGAGGAGAGAGCACGGTACCTGCAGACAATTGTCCAGCACCA TTTAGAGCCAACAACATTTGAAGACTTTGCAGCACAGGTTTTCTCTCCAGCTCCCTACCATCATTTCCCT GCTGACGCAGTTGGATCCTACCCAGAGATTCCACCCAGTGACGCTGCCCCAGCAGCACAGGTAGACGGGG CTGACCTGGCCTCTCCCATGTCTCCTCGAACTAGCAAAAGCCGCATGTCCATGAAGCTGCGTCGGTCCTC TGGCTCAGCCAATAAATCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR220421 representing NM_001003719 Red=Cloning site Green=Tags(s) MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLK QKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQ DNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLSLQETQVTIEEVTPLVPPQSGDKGQEDLTSYF LEALLKYIVIQVKSLEWKNKENQERGFSFLFSHFKKFYLPYIFPNICKENSLYHPVLDIPQIRPKPHYVM IKKDAETNETIYCTKEPFIQARVIVIRWLVSFWLEPKPHSGPNIPGMEGEVLPKNIQRAAASLVSREESK NDTVDKVDKSAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFL LPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEDTAITCSDIPCSETVADHDSAIEDGEKREEENGTSTS EHVRNSSWTKNGSYQEAFHVCEEATEQNIQAGTQAVLQVFIINSSNIFLLEPANEIKNLLDEHTDMCKRI LNIYRYMVVQVSMDKKTWEQMLLVLLRVTESVLKMSSQAFLQFQGKKSMTLAGRLAGPLFQTLIVAWIKA NLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGK GVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRSTRVRH FSQSEDTGNEVFGALHEEQPLPRSSSTSDILEPFTVERAKVNKEDTSPKLPPLNSETGGNSANVPDLMDE FIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPVDDPGVPSEWTSPASAGSSDLMSSDSHS DSFSAFQCEGRKFDNFGFGTDIGIPSSADVDLGSGHHQSTEEQEVASLTTLHLDSETSSLNQQAFSAEVA TVTGSESASPVHSALGSRSQTPSPSTLSRAHIEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGG TLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISADNLTSPSPPVLI PPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTI IKHCSPQFFSLGLPGATMLIMDFIIAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDI AVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKT VAHVACNMLHMLVHYVPRLQIHQPQSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMA LPLKTLLQPVHATGAENDKTEKSVLNCIYKVLHGCVYGAQSFSNPKYFPISLSDLASVDYDPFMHLESLK EPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHY SESTELSPELFESPNIQFFVLNNTTLVSCIQIRSEESMPGGGLAAGLVSANSNVRIIVRDLSGKYSWDSA ILYGPPIVSGLPEPTSFILSMSDQEKPEEPPTSNECLEDIAVKDGLSLQLRRFRETVPTWSTIREEEDVL DELLQYLGTTSPECLQRTGISLNVPAPQPLCISEKQENDVINAILKQYTEEKEFVEKHFNDLNMKASEQD EPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQED KYSILTNIGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNRSTGLTTPYFATSTVEVIFHVSTRMPSDSD DSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGA IVNGKVLPIMVRSTAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHFP ADAVGSYPEIPPSDAAPAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	NM_001003719
ORF Size	6249 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001003719.2, NP_001003719.1
RefSeq Size	7891 bp
RefSeq ORF	6252 bp
Locus ID	56784
UniProt ID	Q6GYP7
MW	234.7 kDa
Gene Summary	Catalytic subunit of the heterodimeric RalGAP1 complex which acts as a GTPase activator for the Ras-like small GTPases RALA and RALB (By similarity). May interact with the HLH region of TCF3/isoform E12.[UniProtKB/Swiss-Prot Function]

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