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ZNF274 (NM_016324) Human Tagged ORF Clone
CAT＃: RC200209
- TrueORF®
ZNF274 (Myc-DDK-tagged)-Human zinc finger protein 274 (ZNF274), transcript variant ZNF274b
ORF Plasmid: tGFP
"NM_016324" in other vectors (4)
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CNY 5,510.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HFB101; ZF2; ZKSCAN19; ZSCAN51 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200209 representing NM_016324
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACATTGAGGTTGTTGAGGTCCTCACACTGAACCAGGAGGTGGCTGGTCCCCGGAATGCCCAGATCC AGGCCCTATATGCTGAAGATGGAAGCCTGAGTGCAGATGCCCCCAGTGAGCAGGTCCAACAGCAGGGCAA GCATCCAGGTGACCCTGAGGCCGCGCGCCAGAGGTTCCGGCAGTTCCGTTATAAGGACATGACAGGTCCC CGGGAGGCCCTGGACCAGCTCCGAGAGCTGTGTCACCAGTGGCTACAGCCTAAGGCACGCTCCAAGGAGC AGATCCTGGAGCTGCTGGTGCTGGAGCAGTTCCTAGGTGCACTGCCTGTGAAGCTCCGGACATGGGTGGA ATCGCAGCACCCAGAGAACTGCCAAGAGGTGGTGGCCCTGGTAGAGGGTGTGACCTGGATGTCTGAGGAG GAAGTACTTCCTGCAGGACAACCTGCCGAGGGCACCACCTGCTGCCTCGAGGTCACTGCCCAGCAGGAGG AGAAGCAGGAGGATGCAGCCATCTGCCCAGTGACAGTGCTCCCTGAGGAGCCAGTGACCTTCCAGGATGT GGCTGTGGACTTCAGCCGGGAGGAGTGGGGGCTGCTGGGCCCGACACAGAGGACCGAGTACCGCGATGTG ATGCTGGAGACCTTTGGGCACCTGGTCTCTGTGGGGTGGGAGACTACACTGGAAAATAAAGAGTTAGCTC CAAATTCTGACATTCCTGAGGAAGAACCAGCCCCCAGCCTGAAAGTACAAGAATCCTCAAGGGATTGTGC CTTGTCCTCTACATTAGAAGATACCTTGCAGGGTGGGGTCCAGGAAGTCCAAGACACAGTGTTGAAGCAG ATGGAGTCTGCTCAGGAAAAAGACCTTCCTCAGAAGAAGCACTTTGACAACCGTGAGTCCCAGGCAAACA GTGGTGCTCTTGACACAAACCAAGTTTCGCTCCAGAAAATTGACAACCCTGAGTCCCAGGCAAACAGTGG CGCTCTTGACACAAACCAAGTTTTGCTCCACAAAATTCCTCCTAGAAAACGATTGCGCAAACGTGACTCA CAAGTTAAAAGTATGAAACATAATTCACGTGTAAAAATTCATCAGAAGAGCTGTGAAAGGCAAAAGGCCA AGGAAGGCAATGGTTGTAGGAAAACCTTCAGTCGGAGTACTAAACAGATTACGTTTATAAGAATTCACAA GGGGAGCCAAGTTTGCCGATGCAGTGAATGTGGTAAAATATTCCGGAACCCAAGATACTTTTCTGTGCAT AAGAAAATCCATACCGGAGAGAGGCCCTATGTGTGTCAAGACTGTGGGAAAGGATTTGTTCAGAGCTCTT CCCTCACACAGCATCAGAGAGTTCATTCTGGAGAGAGACCATTTGAATGTCAGGAGTGTGGGAGGACCTT CAATGATCGCTCAGCCATCTCCCAGCACCTGAGGACTCACACTGGCGCTAAGCCCTACAAGTGTCAGGAC TGTGGAAAAGCCTTCCGCCAGAGCTCCCACCTCATCAGACATCAGAGGACTCACACCGGGGAGCGCCCAT ATGCATGCAACAAATGTGGAAAGGCCTTCACCCAGAGCTCACACCTTATTGGGCACCAGAGAACCCACAA TAGGACAAAGCGAAAGAAGAAACAGCCTACCTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200209 representing NM_016324
Red=Cloning site Green=Tags(s) MNIEVVEVLTLNQEVAGPRNAQIQALYAEDGSLSADAPSEQVQQQGKHPGDPEAARQRFRQFRYKDMTGP REALDQLRELCHQWLQPKARSKEQILELLVLEQFLGALPVKLRTWVESQHPENCQEVVALVEGVTWMSEE EVLPAGQPAEGTTCCLEVTAQQEEKQEDAAICPVTVLPEEPVTFQDVAVDFSREEWGLLGPTQRTEYRDV MLETFGHLVSVGWETTLENKELAPNSDIPEEEPAPSLKVQESSRDCALSSTLEDTLQGGVQEVQDTVLKQ MESAQEKDLPQKKHFDNRESQANSGALDTNQVSLQKIDNPESQANSGALDTNQVLLHKIPPRKRLRKRDS QVKSMKHNSRVKIHQKSCERQKAKEGNGCRKTFSRSTKQITFIRIHKGSQVCRCSECGKIFRNPRYFSVH KKIHTGERPYVCQDCGKGFVQSSSLTQHQRVHSGERPFECQECGRTFNDRSAISQHLRTHTGAKPYKCQD CGKAFRQSSHLIRHQRTHTGERPYACNKCGKAFTQSSHLIGHQRTHNRTKRKKKQPTS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016324 |
| ORF Size | 1644 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016324.3, NP_057408.2 |
| RefSeq Size | 2589 bp |
| RefSeq ORF | 1647 bp |
| Locus ID | 10782 |
| UniProt ID | Q96GC6 |
| Domains | KRAB, LER, zf-C2H2 |
| Protein Families | Transcription Factors |
| Protein Pathways | Neurotrophin signaling pathway |
| MW | 62.1 kDa |
| Gene Summary | This gene encodes a zinc finger protein containing five C2H2-type zinc finger domains, one or two Kruppel-associated box A (KRAB A) domains, and a leucine-rich domain. The encoded protein has been suggested to be a transcriptional repressor. It localizes predominantly to the nucleolus. Alternatively spliced transcript variants encoding different isoforms exist. These variants utilize alternative polyadenylation signals. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200209L3 | Lenti-ORF clone of ZNF274 (Myc-DDK-tagged)-Human zinc finger protein 274 (ZNF274), transcript variant ZNF274b |
CNY 7,410.00 |
|
| RC200209L4 | Lenti-ORF clone of ZNF274 (mGFP-tagged)-Human zinc finger protein 274 (ZNF274), transcript variant ZNF274b |
CNY 7,410.00 |
|
| RG200209 | ZNF274 (tGFP-tagged) - Human zinc finger protein 274 (ZNF274), transcript variant ZNF274b |
CNY 6,080.00 |
|
| SC320278 | ZNF274 (untagged)-Human zinc finger protein 274 (ZNF274), transcript variant ZNF274b |
CNY 4,088.00 |
