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RPB2 (POLR2B) (NM_000938) Human Tagged ORF Clone
CAT＃: RC200440
- TrueORF®
POLR2B (Myc-DDK-tagged)-Human polymerase (RNA) II (DNA directed) polypeptide B, 140kDa (POLR2B)
ORF Plasmid: tGFP
"NM_000938" in other vectors (4)
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CNY 8,176.00
Product images
CNY 300.00
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | hRPB140; POL2RB; RPB2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200440 representing NM_000938
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACGACGCGGATGAGGATATGCAATATGATGAGGATGATGATGAAATCACCCCGGATTTGTGGCAAG AAGCATGCTGGATTGTAATCAGTTCCTATTTTGACGAGAAAGGCTTGGTTAGACAACAGCTGGATTCTTT TGATGAGTTTATTCAGATGTCTGTTCAAAGAATTGTGGAAGACGCTCCTCCTATAGACCTACAGGCTGAA GCTCAGCATGCTAGTGGAGAAGTTGAAGAACCGCCACGATATTTGCTGAAGTTTGAACAAATTTATCTTT CCAAGCCTACCCATTGGGAAAGAGATGGTGCTCCTTCACCAATGATGCCCAATGAAGCTAGATTAAGGAA TCTCACGTATTCTGCTCCGCTTTATGTTGATATAACAAAAACAGTCATTAAAGAAGGTGAAGAACAACTT CAGACTCAGCATCAGAAAACTTTTATAGGAAAAATTCCAATTATGTTGCGGTCAACTTACTGCCTTTTGA ATGGCTTGACAGATCGTGATCTTTGTGAGTTAAATGAATGCCCTTTGGATCCTGGTGGCTATTTCATTAT TAATGGATCAGAAAAGGTTCTGATTGCCCAAGAGAAAATGGCAACAAACACAGTTTATGTGTTTGCCAAA AAGGATTCTAAATATGCCTACACAGGAGAGTGTAGATCATGTCTTGAGAATTCTTCCCGACCCACCAGTA CTATATGGGTTAGCATGCTGGCAAGAGGAGGACAGGGTGCCAAGAAGAGTGCTATTGGTCAGCGCATTGT GGCAACTCTACCATATATCAAGCAAGAAGTTCCCATCATTATTGTGTTCAGAGCATTAGGTTTTGTGTCC GACAGAGATATTTTAGAACATATTATTTATGATTTTGAAGATCCAGAGATGATGGAAATGGTTAAACCTT CTCTCGATGAAGCTTTTGTCATCCAAGAACAGAATGTTGCACTAAATTTCATTGGTTCACGAGGAGCAAA GCCTGGTGTTACTAAAGAGAAAAGAATTAAATATGCAAAGGAAGTTTTACAAAAAGAAATGCTCCCTCAT GTTGGTGTCAGTGATTTTTGTGAGACCAAAAAAGCCTATTTCTTGGGATACATGGTTCATAGGTTACTTC TGGCAGCTTTGGGTAGAAGAGAACTAGATGACAGAGATCACTATGGAAACAAGAGATTGGATCTTGCTGG GCCGCTGCTTGCATTCTTATTTAGAGGTATGTTTAAGAATTTGCTTAAAGAAGTGCGGATCTATGCACAG AAATTTATTGATCGAGGAAAGGATTTTAACTTGGAGTTGGCAATTAAAACACGGATCATATCTGATGGCC TAAAATACTCTTTAGCTACTGGAAACTGGGGTGATCAAAAGAAAGCTCATCAAGCCAGAGCTGGAGTATC TCAGGTGTTAAACCGCCTGACTTTTGCGTCTACTCTTTCTCACCTGCGTCGTTTAAATTCTCCTATTGGT AGAGACGGCAAGCTAGCAAAACCAAGACAGTTGCATAATACGTTGTGGGGAATGGTGTGTCCTGCCGAGA CCCCAGAGGGCCATGCTGTAGGACTTGTGAAGAATTTAGCCTTGATGGCGTATATTTCAGTTGGATCTCA ACCATCTCCAATTCTGGAATTTTTAGAAGAATGGAGTATGGAAAATTTAGAAGAAATTTCTCCTGCAGCT ATTGCTGATGCAACCAAGATTTTTGTTAATGGCTGCTGGGTTGGAATACATAAAGATCCCGAACAACTTA TGAACACCCTAAGGAAATTGAGACGTCAGATGGACATCATTGTGTCTGAAGTTTCTATGATCAGAGATAT TCGAGAGAGGGAGATTCGGATCTATACGGATGCAGGCCGTATTTGTAGACCACTTCTGATTGTGGAAAAA CAAAAGCTACTTTTGAAGAAGAGGCATATTGACCAATTGAAAGAGAGAGAATATAACAACTATAGTTGGC AGGATCTTGTGGCCAGTGGGGTAGTGGAGTATATTGATACCCTGGAAGAAGAAACAGTGATGCTTGCAAT GACTCCAGATGATTTACAGGAGAAAGAAGTAGCTTATTGTTCCACATATACACACTGTGAGATTCATCCC TCAATGATCCTTGGTGTCTGTGCATCTATTATTCCCTTTCCTGATCATAACCAGTCCCCTAGAAACACAT ACCAGTCTGCTATGGGTAAGCAGGCTATGGGAGTTTACATCACCAACTTCCATGTTCGCATGGACACATT GGCCCATGTTCTCTATTATCCTCAAAAGCCACTTGTGACTACACGGTCTATGGAATATCTACGATTTAGA GAGCTGCCAGCAGGCATCAACTCAATTGTGGCCATTGCATCATACACTGGATATAATCAGGAAGACTCTG TTATCATGAATCGTTCAGCTGTAGACCGCGGCTTCTTCAGGTCTGTTTTCTATCGCTCATACAAAGAACA GGAGTCTAAAAAAGGATTTGATCAAGAAGAAGTTTTTGAGAAGCCTACACGTGAAACATGCCAGGGCATG AGGCATGCCATTTACGACAAGCTGGATGATGATGGTTTGATAGCTCCAGGGGTTCGTGTATCAGGAGATG ATGTTATTATAGGCAAAACAGTCACCTTGCCTGAAAATGAAGATGAATTGGAGAGCACCAATAGACGCTA TACCAAGAGAGACTGTAGCACTTTTCTCAGAACTAGTGAGACGGGCATTGTGGATCAGGTTATGGTAACT CTCAATCAGGAAGGATATAAATTTTGTAAAATAAGGGTACGCTCTGTTAGGATTCCACAGATTGGAGACA AATTTGCTAGTCGACATGGTCAAAAGGGTACTTGTGGTATTCAGTATAGACAAGAGGATATGCCTTTCAC CTGTGAAGGTATCACCCCTGATATCATCATCAATCCCCATGCCATCCCCTCTCGTATGACTATTGGTCAC TTAATTGAATGCCTTCAAGGGAAGGTATCGGCTAACAAGGGTGAAATTGGTGATGCCACTCCATTTAATG ATGCTGTTAACGTGCAGAAGATTTCTAATCTTTTATCTGATTATGGCTATCATCTCAGAGGAAATGAGGT CCTGTACAATGGGTTCACTGGTCGAAAAATCACATCACAAATATTTATTGGCCCCACTTATTACCAGCGT TTGAAGCATATGGTGGATGATAAGATTCACTCTCGTGCTAGGGGACCTATTCAGATCCTCAATAGACAGC CCATGGAGGGTAGATCTCGTGATGGTGGCCTGCGTTTTGGAGAAATGGAACGAGATTGTCAGATTGCCCA TGGAGCAGCCCAGTTTTTAAGGGAAAGATTGTTTGAGGCATCAGATCCATATCAGGTTCATGTTTGCAAT CTTTGTGGAATAATGGCGATTGCCAACACCAGGACCCATACATATGAATGCAGGGGCTGCCGCAATAAAA CCCAGATTTCTTTGGTGCGAATGCCTTACGCATGCAAACTATTGTTTCAGGAACTTATGTCTATGAGTAT TGCACCGCGAATGATGAGTGTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200440 representing NM_000938
Red=Cloning site Green=Tags(s) MYDADEDMQYDEDDDEITPDLWQEACWIVISSYFDEKGLVRQQLDSFDEFIQMSVQRIVEDAPPIDLQAE AQHASGEVEEPPRYLLKFEQIYLSKPTHWERDGAPSPMMPNEARLRNLTYSAPLYVDITKTVIKEGEEQL QTQHQKTFIGKIPIMLRSTYCLLNGLTDRDLCELNECPLDPGGYFIINGSEKVLIAQEKMATNTVYVFAK KDSKYAYTGECRSCLENSSRPTSTIWVSMLARGGQGAKKSAIGQRIVATLPYIKQEVPIIIVFRALGFVS DRDILEHIIYDFEDPEMMEMVKPSLDEAFVIQEQNVALNFIGSRGAKPGVTKEKRIKYAKEVLQKEMLPH VGVSDFCETKKAYFLGYMVHRLLLAALGRRELDDRDHYGNKRLDLAGPLLAFLFRGMFKNLLKEVRIYAQ KFIDRGKDFNLELAIKTRIISDGLKYSLATGNWGDQKKAHQARAGVSQVLNRLTFASTLSHLRRLNSPIG RDGKLAKPRQLHNTLWGMVCPAETPEGHAVGLVKNLALMAYISVGSQPSPILEFLEEWSMENLEEISPAA IADATKIFVNGCWVGIHKDPEQLMNTLRKLRRQMDIIVSEVSMIRDIREREIRIYTDAGRICRPLLIVEK QKLLLKKRHIDQLKEREYNNYSWQDLVASGVVEYIDTLEEETVMLAMTPDDLQEKEVAYCSTYTHCEIHP SMILGVCASIIPFPDHNQSPRNTYQSAMGKQAMGVYITNFHVRMDTLAHVLYYPQKPLVTTRSMEYLRFR ELPAGINSIVAIASYTGYNQEDSVIMNRSAVDRGFFRSVFYRSYKEQESKKGFDQEEVFEKPTRETCQGM RHAIYDKLDDDGLIAPGVRVSGDDVIIGKTVTLPENEDELESTNRRYTKRDCSTFLRTSETGIVDQVMVT LNQEGYKFCKIRVRSVRIPQIGDKFASRHGQKGTCGIQYRQEDMPFTCEGITPDIIINPHAIPSRMTIGH LIECLQGKVSANKGEIGDATPFNDAVNVQKISNLLSDYGYHLRGNEVLYNGFTGRKITSQIFIGPTYYQR LKHMVDDKIHSRARGPIQILNRQPMEGRSRDGGLRFGEMERDCQIAHGAAQFLRERLFEASDPYQVHVCN LCGIMAIANTRTHTYECRGCRNKTQISLVRMPYACKLLFQELMSMSIAPRMMSV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000938 |
| ORF Size | 3522 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000938.1, NP_000929.1 |
| RefSeq Size | 3748 bp |
| RefSeq ORF | 3525 bp |
| Locus ID | 5431 |
| UniProt ID | P30876 |
| Domains | RNA_pol_Rpb2_6, RNA_pol_Rpb2_7, RNA_pol_Rpb2_2, RNA_pol_Rpb2_1, RNA_pol_Rpb2_3, RNA_pol_Rpb2_4, RNA_pol_Rpb2_5 |
| Protein Families | Transcription Factors |
| Protein Pathways | Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase |
| MW | 133.7 kDa |
| Gene Summary | This gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptional elongation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200440L3 | Lenti-ORF clone of POLR2B (Myc-DDK-tagged)-Human polymerase (RNA) II (DNA directed) polypeptide B, 140kDa (POLR2B) |
CNY 13,590.00 |
|
| RC200440L4 | Lenti-ORF clone of POLR2B (mGFP-tagged)-Human polymerase (RNA) II (DNA directed) polypeptide B, 140kDa (POLR2B) |
CNY 13,590.00 |
|
| RG200440 | POLR2B (tGFP-tagged) - Human polymerase (RNA) II (DNA directed) polypeptide B, 140kDa (POLR2B) |
CNY 12,830.00 |
|
| SC119548 | POLR2B (untagged)-Human polymerase (RNA) II (DNA directed) polypeptide B, 140kDa (POLR2B) |
CNY 8,184.00 |
