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CHRNB1 (NM_000747) Human Tagged ORF Clone
CAT＃: RC204040
- TrueORF®
CHRNB1 (Myc-DDK-tagged)-Human cholinergic receptor, nicotinic, beta 1 (muscle) (CHRNB1)
ORF Plasmid: tGFP
"NM_000747" in other vectors (6)
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CNY 3,728.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ACHRB; CHRNB; CMS1D; CMS2A; CMS2C; SCCMS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204040 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCCCAGGGGCTCTGCTGATGCTGCTGGGGGCGCTGGGGGCGCCGCTCGCCCCAGGCGTCCGCGGCT CGGAGGCGGAGGGTCGACTCCGGGGGAAACTTTTCTCTGGCTATGATAGCTCCGTGCGGCCAGCGCGGGA GGTGGGAGACCGTGTCAGGGTCAGCGTTGGTCTCATCCTGGCGCAACTCATCAGCCTGAACGAGAAGGAT GAAGAGATGAGCACAAAGGTGTACTTAGACCTGGAGTGGACTGACTACAGGCTGAGCTGGGACCCTGCGG AGCACGACGGCATCGATTCGCTCCGCATCACGGCGGAATCCGTGTGGCTCCCTGACGTGGTGCTACTGAA CAACAATGATGGGAATTTTTACGTGGCTCTGGACATTAGCGTCGTGGTGTCCTCCGACGGCTCCGTGCGT TGGCAACCCCCGGGCATCTATCGCAGCAGCTGCAGCATCCAGGTCACCTACTTCCCCTTCGACTGGCAGA ATTGCACTATGGTGTTCAGCTCCTACAGCTACGACAGCTCGGAGGTCAGCCTGCAGACAGGCCTGGGTCC TGACGGGCAAGGGCATCAGGAAATCCACATTCATGAAGGGACTTTCATTGAGAATGGCCAGTGGGAGATT ATCCACAAGCCCTCTCGGCTAATCCAGCCTCCAGGCGATCCTAGGGGAGGGAGGGAAGGACAGCGCCAGG AAGTCATCTTCTACCTCATCATCCGCCGCAAGCCTCTCTTCTACCTGGTCAACGTCATTGCCCCATGCAT CCTCATCACTCTTCTGGCCATCTTCGTCTTCTACCTGCCACCAGATGCAGGAGAGAAGATGGGGCTCTCA ATCTTTGCCCTGCTGACCCTTACTGTGTTCCTGCTGCTGCTGGCTGACAAAGTACCTGAGACCTCACTAT CAGTACCCATTATTATCAAGTACCTCATGTTTACCATGGTCCTCGTCACCTTCTCAGTCATCCTTAGTGT CGTGGTTCTCAACCTGCACCACCGCTCACCCCACACCCACCAAATGCCCCTTTGGGTCCGTCAGATCTTC ATTCACAAACTTCCGCTGTACCTGCGTCTAAAAAGGCCCAAACCCGAGAGAGACCTGATGCCGGAGCCCC CTCACTGTTCTTCTCCAGGAAGTGGCTGGGGTCGGGGAACAGATGAATATTTCATCCGGAAGCCGCCAAG TGATTTTCTCTTCCCCAAACCCAATAGGTTCCAGCCTGAACTGTCTGCCCCTGATCTGCGGCGATTTATC GATGGTCCAAACCGGGCTGTGGCCCTGCTTCCGGAGCTACGGGAGGTCGTCTCCTCTATCAGCTACATCG CTCGACAGCTGCAGGAACAGGAGGACCACGATGCGCTGAAGGAGGACTGGCAGTTTGTGGCCATGGTAGT GGACCGCCTCTTCCTGTGGACTTTCATCATCTTCACCAGCGTTGGGACCCTAGTCATCTTCCTGGACGCC ACGTACCACTTGCCCCCTCCAGACCCCTTTCCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204040 protein sequence
Red=Cloning site Green=Tags(s) MTPGALLMLLGALGAPLAPGVRGSEAEGRLRGKLFSGYDSSVRPAREVGDRVRVSVGLILAQLISLNEKD EEMSTKVYLDLEWTDYRLSWDPAEHDGIDSLRITAESVWLPDVVLLNNNDGNFYVALDISVVVSSDGSVR WQPPGIYRSSCSIQVTYFPFDWQNCTMVFSSYSYDSSEVSLQTGLGPDGQGHQEIHIHEGTFIENGQWEI IHKPSRLIQPPGDPRGGREGQRQEVIFYLIIRRKPLFYLVNVIAPCILITLLAIFVFYLPPDAGEKMGLS IFALLTLTVFLLLLADKVPETSLSVPIIIKYLMFTMVLVTFSVILSVVVLNLHHRSPHTHQMPLWVRQIF IHKLPLYLRLKRPKPERDLMPEPPHCSSPGSGWGRGTDEYFIRKPPSDFLFPKPNRFQPELSAPDLRRFI DGPNRAVALLPELREVVSSISYIARQLQEQEDHDALKEDWQFVAMVVDRLFLWTFIIFTSVGTLVIFLDA TYHLPPPDPFP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000747 |
| ORF Size | 1503 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000747.3 |
| RefSeq Size | 2437 bp |
| RefSeq ORF | 1506 bp |
| Locus ID | 1140 |
| UniProt ID | P11230 |
| Protein Families | Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane |
| MW | 56.7 kDa |
| Gene Summary | The muscle acetylcholine receptor is composed of five subunits: two alpha subunits and one beta, one gamma, and one delta subunit. This gene encodes the beta subunit of the acetylcholine receptor. The acetylcholine receptor changes conformation upon acetylcholine binding leading to the opening of an ion-conducting channel across the plasma membrane. Mutations in this gene are associated with slow-channel congenital myasthenic syndrome. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204040L1 | Lenti ORF clone of Human cholinergic receptor, nicotinic, beta 1 (muscle) (CHRNB1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204040L2 | Lenti ORF clone of Human cholinergic receptor, nicotinic, beta 1 (muscle) (CHRNB1), mGFP tagged |
CNY 5,890.00 |
|
| RC204040L3 | Lenti ORF clone of Human cholinergic receptor, nicotinic, beta 1 (muscle) (CHRNB1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204040L4 | Lenti ORF clone of Human cholinergic receptor, nicotinic, beta 1 (muscle) (CHRNB1), mGFP tagged |
CNY 5,890.00 |
|
| RG204040 | CHRNB1 (tGFP-tagged) - Human cholinergic receptor, nicotinic, beta 1 (muscle) (CHRNB1) |
CNY 4,370.00 |
|
| SC319546 | CHRNB1 (untagged)-Human cholinergic receptor, nicotinic, beta 1 (muscle) (CHRNB1) |
CNY 2,950.00 |
