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PIAS3 (NM_006099) Human Tagged ORF Clone
CAT＃: RC207034
PIAS3 (Myc-DDK-tagged)-Human protein inhibitor of activated STAT, 3 (PIAS3)
ORF Plasmid: tGFP
"NM_006099" in other vectors (7)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ZMIZ5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207034 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGCTGGGCGAATTAAAGCACATGGTGATGAGTTTCCGGGTGTCTGAGCTCCAGGTGCTTCTTG GCTTTGCTGGCCGGAACAAGAGTGGACGGAAGCACGAGCTCCTGGCCAAGGCTCTGCACCTCCTGAAGTC CAGCTGTGCCCCTAGTGTCCAGATGAAGATCAAAGAGCTTTACCGACGACGCTTTCCCCGGAAGACCCTG GGGCCCTCTGATCTCTCCCTTCTCTCTTTGCCCCCTGGCACCTCTCCTGTAGGCTCCCCTGGTCCTCTAG CTCCCATTCCCCCAACGCTGTTGGCCCCTGGCACCCTGCTGGGCCCCAAGCGTGAGGTGGACATGCACCC CCCTCTGCCCCAGCCTGTGCACCCTGATGTCACCATGAAACCATTGCCCTTCTATGAAGTCTATGGGGAG CTCATCCGGCCCACCACCCTTGCATCCACTTCTAGCCAGCGGTTTGAGGAAGCGCACTTTACCTTTGCCC TCACACCCCAGCAAGTGCAGCAGATTCTTACATCCAGAGAGGTTCTGCCAGGAGCCAAATGTGATTATAC CATACAGGTGCAGCTAAGGTTCTGTCTCTGTGAGACCAGCTGCCCCCAGGAAGATTATTTTCCCCCCAAC CTCTTTGTCAAGGTCAATGGGAAACTGTGCCCCCTGCCGGGTTACCTTCCCCCAACCAAGAATGGGGCCG AGCCCAAGAGGCCCAGCCGCCCCATCAACATCACACCCCTGGCTCGACTCTCAGCCACTGTTCCCAACAC CATTGTGGTCAATTGGTCATCTGAGTTCGGACGGAATTACTCCTTGTCTGTGTACCTGGTGAGGCAGTTG ACTGCAGGAACCCTTCTACAAAAACTCAGAGCAAAGGGTATCCGGAACCCAGACCACTCGCGGGCACTGA TCAAGGAGAAATTGACTGCTGACCCTGACAGTGAGGTGGCCACTACAAGTCTCCGGGTGTCACTCATGTG CCCGCTAGGGAAGATGCGCCTGACTGTCCCTTGTCGTGCCCTCACCTGCGCCCACCTGCAGAGCTTCGAT GCTGCCCTTTATCTACAGATGAATGAGAAGAAGCCTACATGGACATGTCCTGTGTGTGACAAGAAGGCTC CCTATGAATCTCTTATCATTGATGGTTTATTTATGGAGATTCTTAGTTCCTGTTCAGATTGTGATGAGAT CCAATTCATGGAAGATGGATCCTGGTGCCCAATGAAACCCAAGAAGGAGGCATCTGAGGTTTGCCCCCCG CCAGGGTATGGGCTGGATGGCCTCCAGTACAGCCCAGTCCAGGGGGGAGATCCATCAGAGAATAAGAAGA AGGTCGAAGTTATTGACTTGACAATAGAAAGCTCATCAGATGAGGAGGATCTGCCCCCTACCAAGAAGCA CTGTTCTGTCACCTCAGCTGCCATCCCGGCCCTACCTGGAAGCAAAGGAGTCCTGACATCTGGCCACCAG CCATCCTCGGTGCTAAGGAGCCCTGCTATGGGCACGTTGGGTGGGGATTTCCTGTCCAGTCTCCCACTAC ATGAGTACCCACCTGCCTTCCCACTGGGAGCCGACATCCAAGGTTTAGATTTATTTTCATTTCTTCAGAC AGAGAGTCAGCACTATGGCCCCTCTGTCATCACCTCACTAGATGAACAGGATGCCCTTGGCCACTTCTTC CAGTACCGAGGGACCCCTTCTCACTTTCTGGGCCCACTGGCCCCCACGCTGGGGAGCTCCCACTGCAGCG CCACTCCGGCGCCCCCTCCTGGCCGTGTCAGCAGCATTGTGGCCCCTGGGGGGGCCTTGAGGGAGGGGCA TGGAGGACCCCTGCCCTCAGGTCCCTCTTTGACTGGCTGTCGGTCAGACATCATTTCCCTGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207034 protein sequence
Red=Cloning site Green=Tags(s) MAELGELKHMVMSFRVSELQVLLGFAGRNKSGRKHELLAKALHLLKSSCAPSVQMKIKELYRRRFPRKTL GPSDLSLLSLPPGTSPVGSPGPLAPIPPTLLAPGTLLGPKREVDMHPPLPQPVHPDVTMKPLPFYEVYGE LIRPTTLASTSSQRFEEAHFTFALTPQQVQQILTSREVLPGAKCDYTIQVQLRFCLCETSCPQEDYFPPN LFVKVNGKLCPLPGYLPPTKNGAEPKRPSRPINITPLARLSATVPNTIVVNWSSEFGRNYSLSVYLVRQL TAGTLLQKLRAKGIRNPDHSRALIKEKLTADPDSEVATTSLRVSLMCPLGKMRLTVPCRALTCAHLQSFD AALYLQMNEKKPTWTCPVCDKKAPYESLIIDGLFMEILSSCSDCDEIQFMEDGSWCPMKPKKEASEVCPP PGYGLDGLQYSPVQGGDPSENKKKVEVIDLTIESSSDEEDLPPTKKHCSVTSAAIPALPGSKGVLTSGHQ PSSVLRSPAMGTLGGDFLSSLPLHEYPPAFPLGADIQGLDLFSFLQTESQHYGPSVITSLDEQDALGHFF QYRGTPSHFLGPLAPTLGSSHCSATPAPPPGRVSSIVAPGGALREGHGGPLPSGPSLTGCRSDIISLD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006099 |
| ORF Size | 1884 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006099.3, NP_006090.2 |
| RefSeq Size | 2902 bp |
| RefSeq ORF | 1887 bp |
| Locus ID | 10401 |
| UniProt ID | Q9Y6X2 |
| Domains | SAP, zf-MIZ |
| Protein Families | Transcription Factors |
| Protein Pathways | Jak-STAT signaling pathway, Pathways in cancer, Small cell lung cancer, Ubiquitin mediated proteolysis |
| MW | 68 kDa |
| Gene Summary | This gene encodes a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the covalent attachment of a SUMO protein to specific target substrates. It directly binds to several transcription factors and either blocks or enhances their activity. Alternatively spliced transcript variants of this gene have been identified, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207034L1 | Lenti ORF clone of Human protein inhibitor of activated STAT, 3 (PIAS3), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207034L2 | Lenti ORF clone of Human protein inhibitor of activated STAT, 3 (PIAS3), mGFP tagged |
CNY 5,890.00 |
|
| RC207034L3 | Lenti ORF clone of Human protein inhibitor of activated STAT, 3 (PIAS3), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207034L4 | Lenti ORF clone of Human protein inhibitor of activated STAT, 3 (PIAS3), mGFP tagged |
CNY 5,890.00 |
|
| RG207034 | PIAS3 (tGFP-tagged) - Human protein inhibitor of activated STAT, 3 (PIAS3) |
CNY 4,370.00 |
|
| SC116323 | PIAS3 (untagged)-Human protein inhibitor of activated STAT, 3 (PIAS3) |
CNY 2,950.00 |
|
| SC324304 | PIAS3 (untagged)-Human protein inhibitor of activated STAT, 3 (PIAS3) |
CNY 2,950.00 |
