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CDC7 (NM_003503) Human Tagged ORF Clone
CAT＃: RC210742
CDC7 (Myc-DDK-tagged)-Human cell division cycle 7 homolog (S. cerevisiae) (CDC7), transcript variant 1
ORF Plasmid: tGFP
"NM_003503" in other vectors (7)
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CNY 6,408.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CDC7L1; HsCDC7; Hsk1; huCDC7 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210742 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCGTCTTTGGGGATTCAGATGGATGAGCCAATGGCTTTTTCTCCCCAGCGTGACCGGTTTCAGG CTGAAGGCTCTTTAAAAAAAAACGAGCAGAATTTTAAACTTGCAGGTGTTAAAAAAGATATTGAGAAGCT TTATGAAGCTGTACCACAGCTTAGTAATGTGTTTAAGATTGAGGACAAAATTGGAGAAGGCACTTTCAGC TCTGTTTATTTGGCCACAGCACAGTTACAAGTAGGACCTGAAGAGAAAATTGCTCTAAAACACTTGATTC CAACAAGTCATCCTATAAGAATTGCAGCTGAACTTCAGTGCCTAACAGTGGCTGGGGGGCAAGATAATGT CATGGGAGTTAAATACTGCTTTAGGAAGAATGATCATGTAGTTATTGCTATGCCATATCTGGAGCATGAG TCGTTTTTGGACATTCTGAATTCTCTTTCCTTTCAAGAAGTACGGGAATATATGCTTAATCTGTTCAAAG CTTTGAAACGCATTCATCAGTTTGGTATTGTTCACCGTGATGTTAAGCCCAGCAATTTTTTATATAATAG GCGCCTGAAAAAGTATGCCTTGGTAGACTTTGGTTTGGCCCAAGGAACCCATGATACGAAAATAGAGCTT CTTAAATTTGTCCAGTCTGAAGCTCAGCAGGAAAGGTGTTCACAAAACAAATCCCACATAATCACAGGAA ACAAGATTCCACTGAGTGGCCCAGTACCTAAGGAGCTGGATCAGCAGTCCACCACAAAAGCTTCTGTTAA AAGACCCTACACAAATGCACAAATTCAGATTAAACAAGGAAAAGACGGAAAGGAGGGATCTGTAGGCCTT TCTGTCCAGCGCTCTGTTTTTGGAGAAAGAAATTTCAATATACACAGCTCCATTTCACATGAGAGCCCTG CAGTGAAACTCATGAAGCAGTCAAAGACTGTGGATGTACTGTCTAGAAAGTTAGCAACAAAAAAGAAGGC TATTTCTACAAAAGTTATGAATAGTGCTGTGATGAGGAAAACTGCCAGTTCTTGCCCAGCTAGCCTGACC TGTGACTGCTATGCAACAGATAAAGTTTGTAGTATTTGCCTTTCAAGGCGTCAGCAGGTTGCCCCTAGGG CAGGTACACCAGGATTCAGAGCACCAGAGGTCTTGACAAAGTGCCCCAATCAAACTACAGCAATTGACAT GTGGTCTGCAGGTGTCATATTTCTTTCTTTGCTTAGTGGACGATATCCATTTTATAAAGCAAGTGATGAT TTAACTGCTTTGGCCCAAATTATGACAATTAGGGGATCCAGAGAAACTATCCAAGCTGCTAAAACTTTTG GGAAATCAATATTATGTAGCAAAGAAGTTCCAGCACAAGACTTGAGAAAACTCTGTGAGAGACTCAGGGG TATGGATTCTAGCACTCCCAAGTTAACAAGTGATATACAAGGGCATGCTTCTCATCAACCAGCTATTTCA GAGAAGACTGACCATAAAGCTTCTTGCCTCGTTCAAACACCTCCAGGACAATACTCAGGGAATTCATTTA AAAAGGGGGATAGTAATAGCTGTGAGCATTGTTTTGATGAGTATAATACCAATTTAGAAGGCTGGAATGA GGTACCTGATGAAGCTTATGACCTGCTTGATAAACTTCTAGATCTAAATCCAGCTTCAAGAATAACAGCA GAAGAAGCTTTGTTGCATCCATTTTTTAAAGATATGAGCTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210742 protein sequence
Red=Cloning site Green=Tags(s) MEASLGIQMDEPMAFSPQRDRFQAEGSLKKNEQNFKLAGVKKDIEKLYEAVPQLSNVFKIEDKIGEGTFS SVYLATAQLQVGPEEKIALKHLIPTSHPIRIAAELQCLTVAGGQDNVMGVKYCFRKNDHVVIAMPYLEHE SFLDILNSLSFQEVREYMLNLFKALKRIHQFGIVHRDVKPSNFLYNRRLKKYALVDFGLAQGTHDTKIEL LKFVQSEAQQERCSQNKSHIITGNKIPLSGPVPKELDQQSTTKASVKRPYTNAQIQIKQGKDGKEGSVGL SVQRSVFGERNFNIHSSISHESPAVKLMKQSKTVDVLSRKLATKKKAISTKVMNSAVMRKTASSCPASLT CDCYATDKVCSICLSRRQQVAPRAGTPGFRAPEVLTKCPNQTTAIDMWSAGVIFLSLLSGRYPFYKASDD LTALAQIMTIRGSRETIQAAKTFGKSILCSKEVPAQDLRKLCERLRGMDSSTPKLTSDIQGHASHQPAIS EKTDHKASCLVQTPPGQYSGNSFKKGDSNSCEHCFDEYNTNLEGWNEVPDEAYDLLDKLLDLNPASRITA EEALLHPFFKDMSL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003503 |
| ORF Size | 1722 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003503.4 |
| RefSeq Size | 3222 bp |
| RefSeq ORF | 1725 bp |
| Locus ID | 8317 |
| UniProt ID | O00311 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase, Stem cell - Pluripotency |
| Protein Pathways | Cell cycle |
| MW | 63.9 kDa |
| Gene Summary | This gene encodes a cell division cycle protein with kinase activity that is critical for the G1/S transition. The yeast homolog is also essential for initiation of DNA replication as cell division occurs. Overexpression of this gene product may be associated with neoplastic transformation for some tumors. Multiple alternatively spliced transcript variants that encode the same protein have been detected. [provided by RefSeq, Aug 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210742L1 | Lenti ORF clone of Human cell division cycle 7 homolog (S. cerevisiae) (CDC7), transcript variant 1, Myc-DDK-tagged |
CNY 8,808.00 |
|
| RC210742L2 | Lenti ORF clone of Human cell division cycle 7 homolog (S. cerevisiae) (CDC7), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC210742L3 | Lenti ORF clone of Human cell division cycle 7 homolog (S. cerevisiae) (CDC7), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210742L4 | Lenti ORF clone of Human cell division cycle 7 homolog (S. cerevisiae) (CDC7), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG210742 | CDC7 (tGFP-tagged) - Human cell division cycle 7 homolog (S. cerevisiae) (CDC7), transcript variant 1 |
CNY 8,008.00 |
|
| SC126574 | CDC7 (untagged)-Human cell division cycle 7 homolog (S. cerevisiae) (CDC7), transcript variant 1 |
CNY 4,280.00 |
|
| SC323385 | CDC7 (untagged)-Kinase deficient mutant (K90M) of Human cell division cycle 7 homolog (S. cerevisiae) (CDC7), transcript variant 1 |
CNY 4,280.00 |
