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Pyrin (MEFV) (NM_000243) Human Tagged ORF Clone
CAT＃: RC210934
- TrueORF®
MEFV (Myc-DDK-tagged)-Human Mediterranean fever (MEFV), transcript variant 1
ORF Plasmid: tGFP
"NM_000243" in other vectors (6)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | FMF; MEF; PAAND; TRIM20 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210934 representing NM_000243
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTAAGACCCCTAGTGACCATCTGCTGTCCACCCTGGAGGAGCTGGTGCCCTATGACTTCGAGAAGT TCAAGTTCAAGCTGCAGAACACCAGTGTGCAGAAGGAGCACTCCAGGATCCCCCGGAGCCAGATCCAGAG AGCCAGGCCGGTGAAGATGGCCACTCTGCTGGTCACCTACTATGGGGAAGAGTACGCCGTGCAGCTCACC CTGCAGGTCCTGCGGGCCATCAACCAGCGCCTGCTGGCCGAGGAGCTCCACAGGGCAGCCATTCAGGAAT ATTCCACACAAGAAAACGGCACAGACGATTCCGCAGCGTCCAGCTCCCTGGGGGAGAACAAGCCCAGGAG CCTGAAGACTCCAGACCACCCCGAGGGGAACGAGGGGAACGGCCCTCGGCCGTACGGGGGCGGGGCTGCC AGCCTGCGGTGCAGCCAGCCCGAGGCCGGGAGGGGGCTGTCGAGGAAGCCCCTGAGCAAACGCAGAGAGA AGGCATCGGAGGGCCTGGACGCGCAGGGCAAGCCTCGGACCCGGAGCCCGGCCCTGCCGGGCGGGAGAAG CCCCGGCCCCTGCAGGGCGCTAGAGGGGGGCCAGGCCGAGGTCCAGCTGCGCAGAAACGCCAGCTCCGCG GGGAGGCTGCAGGGGCTGGCGGGGGGCGCCCCGGGGCAGAAGGAGTGCAGGCCCTTCGAAGTGTACCTGC CCTCGGGAAAGATGCGACCTAGAAGCCTTGAGGTCACCATTTCTACAGGGGAGAAGGCGCCCGCAAATCC AGAAATTCTCCTGACTCTAGAGGAAAAGACAGCTGCGAATCTGGACTCGGCAACAGAACCCCGGGCAAGG CCCACTCCGGATGGAGGGGCATCTGCGGACCTGAAGGAAGGCCCTGGAAATCCAGAACATTCGGTCACCG GAAGGCCACCAGACACGGCTGCGAGTCCCCGTTGCCACGCCCAGGAAGGAGACCCAGTTGACGGTACCTG TGTGCGTGATTCCTGCAGCTTCCCCGAGGCAGTTTCTGGGCACCCCCAGGCCTCAGGCAGCCGCTCACCT GGCTGCCCCCGGTGCCAGGACTCCCATGAAAGGAAGAGCCCGGGAAGCCTAAGCCCCCAGCCCCTGCCAC AGTGTAAGCGCCACCTGAAGCAGGTCCAGCTGCTCTTCTGTGAGGATCACGATGAGCCCATCTGCCTCAT CTGCAGTCTGAGTCAGGAGCACCAAGGCCACCGGGTGCGCCCCATTGAGGAGGTCGCCCTGGAACACAAG AAGAAAATTCAGAAGCAGCTGGAGCATCTGAAGAAGCTGAGAAAATCAGGGGAGGAGCAGCGATCCTATG GGGAGGAGAAGGCAGTGAGCTTTCTGAAACAAACTGAAGCGCTGAAGCAGCGGGTGCAGAGGAAGCTGGA GCAGGTGTACTACTTCCTGGAACAGCAGGAGCATTTCTTTGTGGCCTCACTGGAGGACGTGGGCCAGATG GTTGGGCAGATCAGGAAGGCATATGACACCCGCGTATCCCAGGACATCGCCCTGCTCGACGCGCTGATTG GGGAACTGGAGGCCAAGGAGTGCCAGTCAGAATGGGAACTTCTGCAGGACATTGGAGACATCTTGCACAG GGCTAAGACAGTGCCTGTCCCTGAAAAGTGGACCACTCCTCAAGAGATAAAACAAAAGATCCAACTCCTC CACCAGAAGTCAGAGTTTGTGGAGAAGAGCACAAAGTACTTCTCAGAAACCCTGCGTTCAGAAATGGAAA TGTTCAATGTTCCGGAGCTGATTGGCGCTCAGGCACATGCTGTTAATGTGATTCTGGATGCAGAAACCGC TTACCCCAACCTCATCTTCTCTGATGATCTGAAGAGTGTTAGACTTGGAAACAAGTGGGAGAGGCTGCCT GATGGCCCGCAAAGATTTGACAGCTGTATCATTGTTCTGGGCTCTCCGAGTTTCCTCTCTGGCCGCCGTT ACTGGGAGGTGGAGGTTGGAGACAAGACAGCATGGATCCTGGGAGCCTGCAAGACATCCATAAGCAGGAA AGGGAACATGACTCTGTCGCCAGAGAATGGCTACTGGGTGGTGATAATGATGAAGGAAAATGAGTACCAG GCGTCCAGCGTTCCCCCGACCCGCCTGCTAATAAAGGAGCCTCCCAAGCGTGTGGGCATCTTCGTGGACT ACAGAGTTGGAAGCATCTCCTTTTACAATGTGACAGCCAGATCCCACATCTATACATTCGCCAGCTGCTC TTTCTCTGGGCCCCTTCAACCTATCTTCAGCCCTGGGACACGTGATGGAGGGAAGAACACAGCTCCTCTG ACTATCTGTCCAGTGGGTGGTCAGGGGCCTGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210934 representing NM_000243
Red=Cloning site Green=Tags(s) MAKTPSDHLLSTLEELVPYDFEKFKFKLQNTSVQKEHSRIPRSQIQRARPVKMATLLVTYYGEEYAVQLT LQVLRAINQRLLAEELHRAAIQEYSTQENGTDDSAASSSLGENKPRSLKTPDHPEGNEGNGPRPYGGGAA SLRCSQPEAGRGLSRKPLSKRREKASEGLDAQGKPRTRSPALPGGRSPGPCRALEGGQAEVQLRRNASSA GRLQGLAGGAPGQKECRPFEVYLPSGKMRPRSLEVTISTGEKAPANPEILLTLEEKTAANLDSATEPRAR PTPDGGASADLKEGPGNPEHSVTGRPPDTAASPRCHAQEGDPVDGTCVRDSCSFPEAVSGHPQASGSRSP GCPRCQDSHERKSPGSLSPQPLPQCKRHLKQVQLLFCEDHDEPICLICSLSQEHQGHRVRPIEEVALEHK KKIQKQLEHLKKLRKSGEEQRSYGEEKAVSFLKQTEALKQRVQRKLEQVYYFLEQQEHFFVASLEDVGQM VGQIRKAYDTRVSQDIALLDALIGELEAKECQSEWELLQDIGDILHRAKTVPVPEKWTTPQEIKQKIQLL HQKSEFVEKSTKYFSETLRSEMEMFNVPELIGAQAHAVNVILDAETAYPNLIFSDDLKSVRLGNKWERLP DGPQRFDSCIIVLGSPSFLSGRRYWEVEVGDKTAWILGACKTSISRKGNMTLSPENGYWVVIMMKENEYQ ASSVPPTRLLIKEPPKRVGIFVDYRVGSISFYNVTARSHIYTFASCSFSGPLQPIFSPGTRDGGKNTAPL TICPVGGQGPD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000243 |
| ORF Size | 2343 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000243.3 |
| RefSeq Size | 3511 bp |
| RefSeq ORF | 2346 bp |
| Locus ID | 4210 |
| UniProt ID | O15553 |
| Protein Families | Druggable Genome |
| Protein Pathways | NOD-like receptor signaling pathway |
| MW | 86.3 kDa |
| Gene Summary | This gene encodes a protein, also known as pyrin or marenostrin, that is an important modulator of innate immunity. Mutations in this gene are associated with Mediterranean fever, a hereditary periodic fever syndrome. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210934L1 | Lenti-ORF clone of MEFV (Myc-DDK-tagged)-Human Mediterranean fever (MEFV), transcript variant 1 |
CNY 5,890.00 |
|
| RC210934L2 | Lenti-ORF clone of MEFV (mGFP-tagged)-Human Mediterranean fever (MEFV), transcript variant 1 |
CNY 5,890.00 |
|
| RC210934L3 | Lenti-ORF clone of MEFV (Myc-DDK-tagged)-Human Mediterranean fever (MEFV), transcript variant 1 |
CNY 5,890.00 |
|
| RC210934L4 | Lenti-ORF clone of MEFV (mGFP-tagged)-Human Mediterranean fever (MEFV), transcript variant 1 |
CNY 5,890.00 |
|
| RG210934 | MEFV (tGFP-tagged) - Human Mediterranean fever (MEFV), transcript variant 1 |
CNY 4,370.00 |
|
| SC300033 | MEFV (untagged)-Human Mediterranean fever (MEFV), transcript variant 1 |
CNY 5,728.00 |
