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Thyrotropin Releasing Hormone (TRH) (NM_007117) Human Tagged ORF Clone
CAT＃: RC219231
TRH (Myc-DDK-tagged)-Human thyrotropin-releasing hormone (TRH)
ORF Plasmid: tGFP
"NM_007117" in other vectors (4)
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CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Pro-TRH; TRF |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC219231 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCGGCCCTTGGTTGCTGCTCGCTCTGGCTTTGACCCTGAACCTGACCGGTGTCCCCGGCGGCCGTG CTCAGCCAGAGGCGGCCCAGCAGGAGGCAGTGACGGCCGCGGAGCATCCGGGCCTGGATGACTTCCTGCG CCAGGTGGAGCGCCTCCTCTTCCTCCGGGAAAACATCCAGCGGCTGCAAGGGGACCAGGGTGAGCACTCC GCGTCCCAGATCTTTCAATCTGACTGGCTCTCCAAACGTCAGCATCCAGGCAAAAGAGAGGAGGAGGAGG AAGAGGGAGTTGAAGAAGAGGAAGAGGAAGAAGGGGGGGCTGTGGGACCCCACAAACGGCAGCACCCTGG CCGACGAGAAGATGAGGCTTCATGGTCAGTCGATGTAACCCAGCACAAGCGGCAGCATCCTGGCCGGCGC TCCCCCTGGCTTGCATATGCTGTCCCGAAGCGGCAGCACCCAGGCAGAAGGCTGGCAGATCCCAAGGCTC AAAGGAGCTGGGAAGAAGAGGAGGAGGAGGAAGAGAGAGAGGAAGACCTGATGCCTGAAAAACGCCAGCA TCCGGGCAAGAGGGCCCTGGGAGGCCCCTGTGGGCCCCAGGGAGCCTATGGTCAAGCGGGCCTCCTGCTG GGGCTCCTGGATGACCTGAGTAGGAGCCAGGGAGCTGAGGAAAAGCGGCAGCACCCTGGTCGGCGGGCAG CCTGGGTCAGGGAGCCCCTGGAGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219231 protein sequence
Red=Cloning site Green=Tags(s) MPGPWLLLALALTLNLTGVPGGRAQPEAAQQEAVTAAEHPGLDDFLRQVERLLFLRENIQRLQGDQGEHS ASQIFQSDWLSKRQHPGKREEEEEEGVEEEEEEEGGAVGPHKRQHPGRREDEASWSVDVTQHKRQHPGRR SPWLAYAVPKRQHPGRRLADPKAQRSWEEEEEEEEREEDLMPEKRQHPGKRALGGPCGPQGAYGQAGLLL GLLDDLSRSQGAEEKRQHPGRRAAWVREPLEE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007117 |
| ORF Size | 726 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007117.1, NP_009048.1 |
| RefSeq Size | 1890 bp |
| RefSeq ORF | 729 bp |
| Locus ID | 7200 |
| UniProt ID | P20396 |
| Protein Families | Druggable Genome, Secreted Protein |
| MW | 27.4 kDa |
| Gene Summary | This gene encodes a member of the thyrotropin-releasing hormone family. Cleavage of the encoded proprotein releases mature thyrotropin-releasing hormone, which is a tripeptide hypothalamic regulatory hormone. The human proprotein contains six thyrotropin-releasing hormone tripeptides. Thyrotropin-releasing hormone is involved in the regulation and release of thyroid-stimulating hormone, as well as prolactin. Deficiency of this hormone has been associated with hypothalamic hypothyroidism. [provided by RefSeq, May 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219231L3 | Lenti ORF clone of Human thyrotropin-releasing hormone (TRH), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC219231L4 | Lenti ORF clone of Human thyrotropin-releasing hormone (TRH), mGFP tagged |
CNY 5,890.00 |
|
| RG219231 | TRH (tGFP-tagged) - Human thyrotropin-releasing hormone (TRH) |
CNY 4,000.00 |
|
| SC115730 | TRH (untagged)-Human thyrotropin-releasing hormone (TRH) |
CNY 2,400.00 |
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