CNOT1 (NM_016284) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AD-005; CDC39; HPE12; NOT1; NOT1H
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC224859 representing NM_016284 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATCTTGACTCGCTCTCGCTGGCCTTGTCTCAAATCAGCTACCTGGTGGACAATTTAACCAAGAAAA ATTACCGAGCCAGCCAGCAGGAAATACAGCATATTGTGAATCGGCACGGTCCTGAGGCAGACAGGCATTT ATTACGCTGCCTATTTTCGCATGTGGATTTCAGTGGCGATGGTAAAAGCAGTGGCAAAGATTTCCATCAG ACTCAGTTTCTGATTCAGGAGTGTGCGTTGCTGATTACAAAGCCAAATTTTATCTCGACGCTGTCCTATG CCATTGATAATCCATTGCACTATCAGAAGAGTTTAAAGCCTGCACCCCACTTATTTGCCCAGCTGAGTAA AGTGCTCAAATTAAGCAAAGTACAAGAGGTAATTTTTGGCCTTGCCCTGTTGAATTCTTCCAGCTCAGAT CTTAGAGGTTTCGCTGCCCAGTTTATCAAACAGAAGCTTCCAGATCTTCTGCGTTCTTACATTGACGCAG ACGTCAGTGGAAATCAAGAAGGTGGCTTCCAAGATATAGCAATAGAGGTCCTACACCTCCTCCTCTCCCA TCTCCTCTTTGGGCAGAAGGGAGCCTTTGGAGTTGGACAAGAACAGATAGACGCTTTTCTTAAGACGCTG CGCAGAGATTTTCCCCAAGAACGCTGTCCCGTGGTGCTCGCACCACTTTTATACCCTGAAAAACGGGACA TTCTAATGGACAGGATCCTGCCTGATTCCGGAGGGGTAGCTAAAACCATGATGGAGAGCTCTTTGGCTGA TTTCATGCAAGAAGTAGGCTATGGCTTTTGTGCAAGTATTGAAGAATGTCGCAATATAATCGTGCAGTTT GGTGTTCGGGAGGTCACAGCTGCCCAGGTTGCAAGGGTTTTGGGAATGATGGCTCGAACTCATTCAGGAT TAACAGATGGCATTCCATTACAGAGTATTTCTGCTCCGGGCAGTGGGATCTGGAGTGATGGGAAAGATAA AAGTGATGGAGCACAGGCACACACATGGAATGTAGAAGTCTTGATTGACGTTCTTAAAGAACTGAATCCA AGTTTGAATTTCAAGGAAGTAACTTATGAACTGGACCATCCTGGATTTCAAATTCGTGACAGTAAAGGAC TTCATAATGTGGTTTATGGCATTCAGAGGGGTTTGGGTATGGAAGTGTTCCCAGTAGACCTCATATATAG ACCTTGGAAACATGCTGAAGGCCAGCTCTCCTTCATTCAACATTCCCTTATAAATCCAGAGATCTTCTGT TTTGCTGACTATCCCTGTCATACTGTTGCCACTGATATTCTGAAAGCACCACCAGAGGATGACAATCGAG AAATTGCCACATGGAAGAGCTTGGATTTGATTGAATCTCTGCTGAGGCTTGCAGAGGTTGGGCAGTATGA GCAAGTCAAACAGCTCTTCAGCTTCCCTATCAAACACTGTCCAGACATGCTGGTATTGGCCTTACTACAA ATTAACACCTCTTGGCATACCTTGCGCCATGAACTTATCTCCACTCTGATGCCAATTTTCCTTGGAAACC ATCCTAACTCAGCTATTATTTTGCACTATGCATGGCATGGGCAGGGACAGTCTCCCTCAATTCGCCAACT TATCATGCATGCAATGGCAGAATGGTACATGAGAGGGGAGCAGTATGATCAGGCCAAATTGTCTCGAATA CTTGATGTGGCCCAGGACTTGAAGGCCTTGTCAATGCTGCTAAATGGTACTCCATTTGCCTTTGTTATTG ACCTTGCTGCACTTGCTTCACGTCGTGAATACCTCAAACTTGATAAGTGGCTCACAGATAAAATTCGAGA GCATGGGGAGCCTTTTATCCAGGCGTGTATGACTTTTTTAAAGAGACGGTGTCCTTCTATTTTGGGCGGA CTTGCCCCAGAAAAAGACCAGCCCAAAAGTGCTCAACTTCCTCCAGAAACTTTGGCGACAATGTTGGCCT GTCTGCAAGCTTGTGCAGGGAGTGTTTCTCAGGAGCTATCAGAAACTATCCTCACCATGGTAGCCAATTG CAGTAATGTTATGAATAAGGCCAGACAACCACCACCTGGAGTTATGCCAAAAGGACGTCCTCCTAGTGCT AGCAGCTTAGATGCCATTTCTCCTGTTCAGATTGACCCTCTTGCTGGAATGACATCTCTTAGTATAGGTG GTTCAGCTGCCCCTCACACCCAGAGTATGCAGGGTTTTCCTCCAAATTTGGGTTCTGCATTCAGTACCCC TCAGTCACCAGCAAAAGCATTTCCACCCCTTTCAACCCCCAATCAGACCACTGCATTCAGTGGTATTGGA GGACTTTCATCACAGCTTCCAGTAGGTGGTCTTGGCACAGGCAGCCTGACTGGTATAGGAACTGGTGCTC TTGGACTCCCTGCAGTGAATAACGACCCTTTTGTACAGAGGAAACTGGGCACCTCTGGACTGAATCAGCC TACATTCCAGCAGAGTAAGATGAAACCTTCGGACTTGTCTCAGGTGTGGCCAGAGGCAAACCAGCACTTT AGTAAAGAGATAGATGATGAAGCAAACAGCTATTTCCAGCGAATATATAATCATCCACCACATCCAACCA TGTCTGTTGATGAGGTATTAGAAATGCTGCAGAGATTTAAAGACTCTACTATAAAGAGGGAACGAGAAGT ATTTAACTGTATGCTAAGGAACTTGTTTGAAGAATATCGTTTTTTTCCCCAGTATCCTGATAAAGAGTTA CATATAACAGCCTGCCTATTTGGTGGTATAATTGAGAAAGGACTGGTCACTTACATGGCACTAGGTCTGG CTCTACGATATGTTCTTGAAGCCTTACGCAAGCCTTTTGGATCCAAAATGTATTATTTCGGGATTGCTGC ACTAGATAGATTTAAAAACAGATTGAAGGACTATCCCCAGTATTGTCAGCATTTGGCTTCTATCAGTCAC TTTATGCAATTTCCACATCATTTACAGGAGTATATTGAGTATGGACAGCAGTCTAGAGATCCTCCTGTGA AAATGCAAGGCTCTATCACAACCCCTGGAAGTATTGCACTGGCTCAGGCCCAGGCTCAGGCCCAGGTTCC AGCAAAAGCTCCTCTTGCTGGTCAAGTTAGCACTATGGTAACCACCTCAACAACTACCACTGTTGCTAAA ACGGTTACGGTCACCAGGCCAACTGGAGTCAGCTTTAAGAAAGATGTGCCACCTTCTATTAATACTACAA ATATAGATACGTTGCTTGTGGCCACAGATCAAACTGAGAGAATTGTGGAGCCCCCAGAAAATATCCAGGA GAAAATTGCTTTTATTTTCAATAATCTCTCACAGTCAAATATGACACAAAAGGTTGAAGAGCTAAAGGAA ACGGTGAAAGAAGAATTTATGCCTTGGGTTTCACAGTATCTGGTTATGAAGAGAGTCAGTATTGAGCCAA ACTTTCATAGCCTGTATTCAAACTTCCTTGACACGCTGAAGAATCCTGAATTTAACAAGATGGTTCTGAA TGAGACCTACAGAAACATTAAAGTGCTCCTGACCTCTGATAAAGCTGCAGCCAATTTCTCAGATCGTTCT TTGCTGAAGAACTTGGGACATTGGCTAGGAATGATCACATTAGCTAAAAACAAACCCATCTTACACACTG ACTTGGATGTGAAATCATTGCTGCTAGAGGCTTATGTTAAAGGACAACAAGAATTGCTCTATGTAGTGCC CTTTGTTGCCAAAGTCTTAGAATCTAGCATTCGTAGTGTGGTTTTTAGGCCACCAAACCCTTGGACAATG GCAATTATGAATGTATTAGCTGAGCTACATCAGGAGCATGACTTAAAGTTAAACTTGAAGTTTGAAATCG AGGTTCTCTGCAAGAACCTTGCATTAGACATCAATGAGCTAAAACCTGGAAACCTCCTAAAGGATAAAGA TCGCCTGAAGAATTTAGATGAGCAACTCTCTGCTCCAAAGAAAGATGTCAAGCAGCCAGAAGAACTCCCT CCCATCACAACCACAACAACTTCTACTACACCAGCTACCAACACCACTTGTACAGCCACGGTTCCACCAC AGCCACAGTACAGCTACCACGACATCAATGTCTATTCCCTTGCGGGCTTGGCACCACACATTACTCTGAA TCCAACAATTCCCTTGTTTCAGGCCCATCCACAGTTGAAGCAGTGTGTGCGTCAGGCAATTGAACGGGCT GTCCAGGAGCTGGTCCATCCTGTGGTGGATCGATCAATTAAGATTGCCATGACTACTTGTGAGCAAATAG TCAGGAAGGATTTTGCCCTGGATTCGGAGGAATCTCGAATGCGAATAGCAGCTCATCACATGATGCGTAA CTTGACAGCTGGAATGGCTATGATTACATGCAGGGAACCTTTGCTCATGAGCATATCTACCAACTTAAAA AACAGTTTTGCCTCAGCCCTTCGTACTGCTTCCCCACAACAAAGAGAAATGATGGATCAGGCAGCTGCTC AATTAGCTCAGGACAATTGTGAGTTGGCTTGCTGTTTTATTCAGAAGACTGCAGTAGAAAAAGCAGGCCC TGAGATGGACAAGAGATTAGCAACTGAATTTGAGCTGAGAAAACATGCTAGGCAAGAAGGACGCAGATAC TGTGATCCTGTTGTTTTAACATATCAAGCTGAACGGATGCCAGAGCAAATCAGGCTGAAAGTTGGTGGTG TGGACCCAAAGCAGTTGGCTGTTTACGAAGAGTTTGCACGCAATGTTCCTGGCTTCTTACCTACAAATGA CTTAAGTCAGCCCACGGGATTTTTAGCCCAGCCCATGAAGCAAGCTTGGGCAACAGATGATGTAGCTCAG ATTTATGATAAGTGTATTACAGAACTGGAGCAACATCTACATGCCATCCCACCAACTTTGGCCATGAACC CTCAAGCTCAGGCTCTTCGAAGTCTCTTGGAGGTTGTAGTTTTATCTCGAAACTCTCGGGATGCCATAGC TGCTCTTGGATTGCTCCAAAAGGCTGTAGAGGGCTTACTAGATGCCACAAGTGGTGCTGATGCTGACCTT CTGCTGCGCTACAGGGAATGCCACCTCTTGGTCCTAAAAGCTCTGCAGGATGGCCGGGCATATGGGTCTC CATGGTGCAACAAACAGATCACAAGGTGCCTAATTGAATGTCGAGATGAATATAAATATAATGTGGAGGC TGTGGAGCTGCTAATTCGCAATCATTTGGTTAATATGCAGCAGTATGATCTTCACCTAGCGCAGTCAATG GAGAATGGCTTAAACTACATGGCTGTGGCATTTGCTATGCAGTTAGTAAAAATCCTGCTGGTGGATGAAA GGAGTGTTGCTCATGTTACTGAGGCAGATCTGTTCCACACCATTGAAACCCTCATGAGGATTAATGCTCA TTCCAGAGGCAATGCTCCAGAAGGATTGCCCCAGCTGATGGAAGTAGTGCGATCCAACTATGAAGCAATG ATTGATCGTGCTCATGGAGGCCCAAACTTTATGATGCATTCTGGGATCTCTCAAGCCTCAGAGTATGATG ACCCTCCAGGCCTGAGGGAGAAGGCAGAGTATCTTCTGAGGGAATGGGTGAATCTCTACCATTCAGCAGC AGCTGGCCGCGACAGTACCAAAGCTTTCTCTGCATTTGTTGGACAGATGCACCAGCAAGGAATACTGAAG ACCGATGATCTCATAACAAGGTTCTTTCGTCTGTGTACTGAAATGTGTGTTGAAATCAGTTACCGTGCTC AGGCTGAGCAGCAGCACAATCCTGCTGCCAATCCCACCATGATCCGAGCCAAGTGCTATCACAACCTGGA TGCCTTTGTTCGACTCATTGCACTGCTCGTGAAACACTCAGGGGAGGCCACCAACACTGTCACAAAGATT AATCTGCTGAACAAGGTCCTTGGTATAGTAGTGGGAGTTCTCCTTCAGGATCATGATGTTCGTCAGAGTG AATTTCAGCAACTTCCCTACCATCGAATTTTTATCATGCTTCTCTTGGAACTCAATGCACCTGAGCATGT GTTGGAAACCATTAATTTCCAGACACTTACAGCTTTCTGCAATACATTCCACATCTTGAGGCCTACCAAA GCTCCTGGCTTTGTATATGCCTGGCTTGAACTGATTTCCCATCGGATATTTATTGCAAGAATGCTGGCAC ATACGCCACAGCAGAAGGGGTGGCCTATGTATGCACAGCTACTGATTGATTTATTCAAATATTTAGCGCC TTTCCTTAGAAATGTGGAACTCACCAAACCTATGCAAATCCTCTACAAGGGCACTTTAAGAGTGCTGCTG GTTCTTTTGCATGATTTCCCAGAGTTCCTTTGTGATTACCATTATGGGTTCTGTGATGTGATCCCACCTA ATTGTATCCAGTTAAGAAATTTGATCCTGAGTGCCTTTCCAAGAAACATGAGGCTCCCCGACCCATTCAC TCCTAATCTAAAGGTGGACATGTTGAGTGAAATTAACATTGCTCCCCGGATTCTCACCAATTTCACTGGA GTAATGCCACCTCAGTTCAAAAAGGATTTGGATTCCTATCTTAAAACTCGATCACCAGTCACTTTCCTGT CTGATCTGCGCAGCAACCTACAGGTATCCAATGAACCTGGGAATCGCTACAACCTCCAGCTCATCAATGC ACTGGTGCTCTATGTCGGGACTCAGGCCATTGCGCACATCCACAACAAGGGCAGCACACCTTCAATGAGC ACCATCACTCACTCAGCACACATGGATATCTTCCAGAATTTGGCTGTGGACTTGGACACTGAGGGTCGCT ATCTCTTTTTGAATGCAATTGCAAATCAGCTCCGGTACCCAAATAGCCACACTCACTACTTCAGTTGCAC CATGCTGTACCTTTTTGCAGAGGCCAATACGGAAGCCATCCAAGAACAGATCACAAGAGTTCTCTTGGAA CGGTTGATTGTAAATAGGCCACATCCTTGGGGTCTTCTTATTACCTTCATTGAGCTGATTAAAAACCCAG CGTTTAAGTTCTGGAACCATGAATTTGTACACTGTGCCCCAGAAATCGAAAAGTTATTCCAGTCGGTCGC ACAGTGCTGCATGGGACAGAAGCAGGCCCAGCAAGTAATGGAAGGGACAGGTGCCAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC224859 representing NM_016284 Red=Cloning site Green=Tags(s) MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQ TQFLIQECALLITKPNFISTLSYAIDNPLHYQKSLKPAPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSD LRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTL RRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGVAKTMMESSLADFMQEVGYGFCASIEECRNIIVQF GVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNP SLNFKEVTYELDHPGFQIRDSKGLHNVVYGIQRGLGMEVFPVDLIYRPWKHAEGQLSFIQHSLINPEIFC FADYPCHTVATDILKAPPEDDNREIATWKSLDLIESLLRLAEVGQYEQVKQLFSFPIKHCPDMLVLALLQ INTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHAMAEWYMRGEQYDQAKLSRI LDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRRCPSILGG LAPEKDQPKSAQLPPETLATMLACLQACAGSVSQELSETILTMVANCSNVMNKARQPPPGVMPKGRPPSA SSLDAISPVQIDPLAGMTSLSIGGSAAPHTQSMQGFPPNLGSAFSTPQSPAKAFPPLSTPNQTTAFSGIG GLSSQLPVGGLGTGSLTGIGTGALGLPAVNNDPFVQRKLGTSGLNQPTFQQSKMKPSDLSQVWPEANQHF SKEIDDEANSYFQRIYNHPPHPTMSVDEVLEMLQRFKDSTIKREREVFNCMLRNLFEEYRFFPQYPDKEL HITACLFGGIIEKGLVTYMALGLALRYVLEALRKPFGSKMYYFGIAALDRFKNRLKDYPQYCQHLASISH FMQFPHHLQEYIEYGQQSRDPPVKMQGSITTPGSIALAQAQAQAQVPAKAPLAGQVSTMVTTSTTTTVAK TVTVTRPTGVSFKKDVPPSINTTNIDTLLVATDQTERIVEPPENIQEKIAFIFNNLSQSNMTQKVEELKE TVKEEFMPWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFNKMVLNETYRNIKVLLTSDKAAANFSDRS LLKNLGHWLGMITLAKNKPILHTDLDVKSLLLEAYVKGQQELLYVVPFVAKVLESSIRSVVFRPPNPWTM AIMNVLAELHQEHDLKLNLKFEIEVLCKNLALDINELKPGNLLKDKDRLKNLDEQLSAPKKDVKQPEELP PITTTTTSTTPATNTTCTATVPPQPQYSYHDINVYSLAGLAPHITLNPTIPLFQAHPQLKQCVRQAIERA VQELVHPVVDRSIKIAMTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTAGMAMITCREPLLMSISTNLK NSFASALRTASPQQREMMDQAAAQLAQDNCELACCFIQKTAVEKAGPEMDKRLATEFELRKHARQEGRRY CDPVVLTYQAERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPTNDLSQPTGFLAQPMKQAWATDDVAQ IYDKCITELEQHLHAIPPTLAMNPQAQALRSLLEVVVLSRNSRDAIAALGLLQKAVEGLLDATSGADADL LLRYRECHLLVLKALQDGRAYGSPWCNKQITRCLIECRDEYKYNVEAVELLIRNHLVNMQQYDLHLAQSM ENGLNYMAVAFAMQLVKILLVDERSVAHVTEADLFHTIETLMRINAHSRGNAPEGLPQLMEVVRSNYEAM IDRAHGGPNFMMHSGISQASEYDDPPGLREKAEYLLREWVNLYHSAAAGRDSTKAFSAFVGQMHQQGILK TDDLITRFFRLCTEMCVEISYRAQAEQQHNPAANPTMIRAKCYHNLDAFVRLIALLVKHSGEATNTVTKI NLLNKVLGIVVGVLLQDHDVRQSEFQQLPYHRIFIMLLLELNAPEHVLETINFQTLTAFCNTFHILRPTK APGFVYAWLELISHRIFIARMLAHTPQQKGWPMYAQLLIDLFKYLAPFLRNVELTKPMQILYKGTLRVLL VLLHDFPEFLCDYHYGFCDVIPPNCIQLRNLILSAFPRNMRLPDPFTPNLKVDMLSEINIAPRILTNFTG VMPPQFKKDLDSYLKTRSPVTFLSDLRSNLQVSNEPGNRYNLQLINALVLYVGTQAIAHIHNKGSTPSMS TITHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANTEAIQEQITRVLLE RLIVNRPHPWGLLITFIELIKNPAFKFWNHEFVHCAPEIEKLFQSVAQCCMGQKQAQQVMEGTGAS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_016284
ORF Size	7128 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_016284.5
RefSeq Size	8449 bp
RefSeq ORF	7131 bp
Locus ID	23019
UniProt ID	A5YKK6
Domains	Not1
Protein Pathways	RNA degradation
MW	266.8 kDa
Gene Summary	Scaffolding component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Its scaffolding function implies its interaction with the catalytic complex module and diverse RNA-binding proteins mediating the complex recruitment to selected mRNA 3' UTRs. Involved in degradation of AU-rich element (ARE)-containing mRNAs probably via association with ZFP36. Mediates the recruitment of the CCR4-NOT complex to miRNA targets and to the RISC complex via association with TNRC6A, TNRC6B or TNRC6C. Acts as a transcriptional repressor. Represses the ligand-dependent transcriptional activation by nuclear receptors. Involved in the maintenance of embryonic stem (ES) cell identity.[UniProtKB/Swiss-Prot Function]

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