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PHF10 (NM_133325) Human Tagged ORF Clone
CAT＃: RC229249
- TrueORF®
PHF10 (Myc-DDK-tagged)-Human PHD finger protein 10 (PHF10), transcript variant 2
ORF Plasmid: tGFP
"NM_133325" in other vectors (5)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BAF45A; XAP135 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC229249 representing NM_133325.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGGCGGCGGCCGGGCCCGGGGCTGCGCTGTCCCCGCGGCCGTGTGACAGCGACCCAGCCACCCCC GGAGCGCAGTCCCCGAAGGATGATAATGAAGATAATTCAAATGATGGGACCCAGCCATCCAAAAGGAGG CGAATGGGCTCAGGAGATAGTTCTAGGAGTTGTGAAACTTCAAGTCAAGATCTTGGTTTTAGTTACTAT CCAGCAGAAAACTTGATAGAGTACAAATGGCCACCTGATGAAACAGGAGAATACTATATGCTTCAAGAA CAAGTCAGTGAATATTTGGGTGTGACCTCCTTTAAAAGGAAATATCCAGAGCGACGAGATTTGTCTCAC AAGGAGAAACTCTACCTGAGAGAGCTAAATGTCATTACTGAAACTCAGTGCACTCTAGGCTTAACAGCA TTGCGCAGTGATGAAGTGATTGATTTAATGATAAAAGAATATCCAGCCAAACATGCTGAGTATTCTGTT ATTCTACAAGAAAAAGAACGTCAACGAATTACAGACCATTATAAAGAGTATTCCCAAATGCAACAACAG AATACTCAGAAAGTTGAAGCCAGTAAAGTGCCTGAGTATATTAAGAAAGCTGCCAAAAAAGCAGCAGAA TTTAATAGCAACTTAAACCGGGAACGCATGGAAGAAAGAAGAGCTTATTTTGACTTGCAGACACATGTT ATCCAGGTACCTCAAGGGAAGTACAAAGTTTTGCCAACAGAGCGAACAAAGGTCAGTTCTTACCCAGTG GCTCTCATCCCCGGACAGTTCCAGGAATATTATAAGAGGTACTCACCAGATGAGCTGCGGTATCTGCCA TTAAACACAGCCCTGTATGAGCCCCCTCTGGATCCTGAGCTCCCTGCTCTAGACAGTGATGGTGATTCA GATGATGGCGAAGATGGTCGAGGTGATGAGAAACGGAAAAATAAAGGCACTTCGGACAGCTCCTCTGGC AATGTATCTGAAGGGGAAAGCCCTCCTGACAGCCAGGAGGACTCTTTCCAGGGAAGACAGAAATCAAAA GACAAAGCTGCCACTCCAAGAAAAGATGGTCCCAAACGTTCTGTACTGTCCAAGTCAGTTCCTGGGTAC AAGCCAAAGGTCATTCCAAATGCTATATGTGGAATTTGTCTGAAGGGTAAGGAGTCCAACAAGAAAGGA AAGGCTGAATCACTTATACACTGCTCCCAATGTGAGAATAGTGGCCATCCTTCTTGCCTGGATATGACA ATGGAGCTTGTTTCTATGATTAAGACCTACCCATGGCAGTGTATGGAATGTAAAACATGCATTATATGT GGACAACCCCACCATGAAGAAGAAATGATGTTCTGTGATATGTGTGACAGAGGTTATCATACTTTTTGT GTGGGCCTTGGTGCTATTCCATCAGGTCGCTGGATTTGTGACTGTTGTCAGCGGGCCCCCCCAACACCC AGGAAAGTGGGCAGAAGGGGGAAAAACAGCAAAGAGGGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC >Peptide sequence encoded by RC229249
Blue=ORF Red=Cloning site Green=Tag(s) MAAAAGPGAALSPRPCDSDPATPGAQSPKDDNEDNSNDGTQPSKRRRMGSGDSSRSCETSSQDLGFSYY PAENLIEYKWPPDETGEYYMLQEQVSEYLGVTSFKRKYPERRDLSHKEKLYLRELNVITETQCTLGLTA LRSDEVIDLMIKEYPAKHAEYSVILQEKERQRITDHYKEYSQMQQQNTQKVEASKVPEYIKKAAKKAAE FNSNLNRERMEERRAYFDLQTHVIQVPQGKYKVLPTERTKVSSYPVALIPGQFQEYYKRYSPDELRYLP LNTALYEPPLDPELPALDSDGDSDDGEDGRGDEKRKNKGTSDSSSGNVSEGESPPDSQEDSFQGRQKSK DKAATPRKDGPKRSVLSKSVPGYKPKVIPNAICGICLKGKESNKKGKAESLIHCSQCENSGHPSCLDMT MELVSMIKTYPWQCMECKTCIICGQPHHEEEMMFCDMCDRGYHTFCVGLGAIPSGRWICDCCQRAPPTP RKVGRRGKNSKEG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133325 |
| ORF Size | 1488 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq Size | 1686 bp |
| RefSeq ORF | 1491 bp |
| Locus ID | 55274 |
| UniProt ID | Q8WUB8 |
| Domains | PHD |
| Protein Families | Druggable Genome, Stem cell - Pluripotency, Transcription Factors |
| MW | 55.8 kDa |
| Gene Summary | This gene contains a predicted ORF that encodes a protein with two zinc finger domains. The function of the encoded protein is not known. Sequence analysis suggests that multiple alternatively spliced transcript variants are derived from this gene but the full-length nature of only two of them is known. These two splice variants encode different isoforms. A pseudogene for this gene is located on Xq28. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229249L3 | Lenti-ORF clone of PHF10 (Myc-DDK-tagged)-Human PHD finger protein 10 (PHF10), transcript variant 2 |
CNY 5,890.00 |
|
| RC229249L4 | Lenti-ORF clone of PHF10 (mGFP-tagged)-Human PHD finger protein 10 (PHF10), transcript variant 2 |
CNY 5,890.00 |
|
| RG229249 | PHF10 (tGFP-tagged) - Human PHD finger protein 10 (PHF10), transcript variant 2 |
CNY 4,370.00 |
|
| SC124594 | PHF10 (untagged)-Human PHD finger protein 10 (PHF10), transcript variant 2 |
CNY 7,220.00 |
|
| SC327884 | PHF10 (untagged)-Human PHD finger protein 10 (PHF10) transcript variant 2 |
CNY 7,220.00 |
