TNIP1 (NM_001258454) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ABIN-1; NAF1; nip40-1; VAN
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234626 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC RCATGGAAGGGAGAGGACCGTACCGGATCTACGACCCTGGGGGCAGCGTGCCCTCAGGAGAGGCATCCGC AGCTTTTGAGCGCCTAGTGAAGGAGAATTCCCGGCTGAAGGAAAAAATGCAAGGGATAAAGATGTTAGGG GAGCTTTTGGAAGAGTCCCAGATGGAAGCGACCAGGCTCCGGCAGAAGGCAGAGGAGCTAGTGAAGGACA ACGAGCTGCTCCCACCACCTTCTCCCTCCTTGGGCTCCTTCGACCCCCTGGCTGAGCTCACAGGAAAGGA CTCAAATGTCACAGCATCTCCCACAGCCCCTGCATGCCCCAGTGACAAGCCAGCACCAGTCCAGAAGCCT CCATCCAGTGGCACCTCCTCTGAATTTGAAGTGGTCACTCCTGAGGAGCAGAATTCACCAGAGAGCAGCA GCCATGCCAATGCGATGGCGCTGGACCCCCTGCCCCGTGAGGACGGCAACCTGATGCTGCACCTGCAGCG CCTGGAGACCACGCTGAGTGTGTGTGCCGAGGAGCCGGACCACGGCCAGCTCTTCACCCACCTGGGCCGC ATGGCCCTGGAGTTCAACCGACTGGCATCCAAGGTGCACAAGAATGAGCAGCGCACCTCCATTCTGCAGA CCCTGTGTGAGCAGCTTCGGAAGGAGAACGAGGCTCTGAAGGCCAAGTTGGATAAGGGCCTGGAACAGCG GGATCAGGCTGCCGAGAGGCTGCGGGAGGAAAATTTGGAGCTCAAGAAGTTGTTGATGAGCAATGGCAAC AAAGAGGGTGCGTCTGGGCGGCCAGGCTCACCGAAGATGGAAGGGACAGGCAAGAAGGCAGTGGCTGGAC AGCAGCAGGCTAGTGTGACGGCAGGTAAGGTCCCAGAGGTGGTGGCCTTGGGCGCAGCCGAGAAGAAGGT GAAGATGCTGGAGCAGCAGCGCAGTGAGCTGCTGGAAGTGAACAAGCAGTGGGACCAGCATTTCCGGTCC ATGAAGCAGCAGTATGAGCAGAAGATCACTGAGCTGCGTCAGAAGCTGGCTGATTTGCAGAAGCAGGTGA CTGACCTGGAGGCCGAGCGGGAGCAGAAGCAGCGTGACTTTGACCGCAAGCTCCTCCTGGCCAAGTCCAA GATTGAAATGGAGGAGACCGACAAGGAGCAGCTGACAGCAGAGGCCAAGGAGCTGCGCCAAAAGGTCAAG TACCTGCAGGATCAGCTGAGCCCACTCACCCGACAGCGTGAGTACCAGGAAAAGGAGATCCAGCGGCTCA ACAAGGCCCTGGAGGAAGCACTGAGCATCCAAACCCCGCCATCATCTCCACCAACAGCATTTGGGAGCCC AGAAGGAGCAGGGGCCCTCCTAAGGAAACAGGAGCTGGTCACGCAGAATGAGTTGCTGAAACAGCAGGTG AAGATCTTCGAGGAGGACTTCCAGAGGGAGCGCAGTGATCGTGAGCGCATGAATGAGGAGAAGGAAGAGC TGAAGAAGCAAGTGGAGAAGCTGCAGGCCCAGGTCACCCTGTCAAATGCCCAGCTAAAAGCATTCAAAGA TGAGGAGAAGGCAAGAGAAGCCCTCAGACAGCAGAAGAGGAAAGCAAAGGCCTCAGGAGAGCGTTACCAT GTGGAGCCCCACCCAGAACATCTCTGCGGGGCCTACCCCTACGCCTACCCGCCCATGCCAGCCATGGTGC CACACCATGGCTTCGAGGACTGGTCCCAGATCCGCTACCCCCCTCCCCCCATGGCCATGGAGCACCCGCC CCCACTCCCCAACTCGCGCCTCTTCCATCTGCCGGAATACACCTGGCGTCTACCCTGTGGAGGGGTTCGA AATCCAAATCAGAGCTCCCAAGTGATGGACCCTCCCACAGCCAGGCCTACAGAACCAGAGTCTCCAAAAA ATGACCGTGAGGGGCCTCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234626 protein sequence Red=Cloning site Green=Tags(s) XWKGEDRTGSTTLGAACPQERHPQLLSA**RRIPG*RKKCKG*RC*GSFWKSPRWKRPGSGRRQRS**RT TSCSHHLLPPWAPSTPWLSSQERTQMSQHLPQPLHAPVTSQHQSRSLHPVAPPLNLKWSLLRSRIHQRAA AMPMRWRWTPCPVRTAT*CCTCSAWRPR*VCVPRSRTTASSSPTWAAWPWSSTDWHPRCTRMSSAPPFCR PCVSSFGRRTRL*RPSWIRAWNSGIRLPRGCGRKIWSSRSC**AMATKRVRLGGQAHRRWKGQARRQWLD SSRLV*RQVRSQRWWPWAQPRRR*RCWSSSAVSCWK*TSSGTSISGP*SSSMSRRSLSCVRSWLICRSR* LTWRPSGSRSSVTLTASSSWPSPRLKWRRPTRSS*QQRPRSCAKRSSTCRIS*AHSPDSVSTRKRRSSGS TRPWRKH*ASKPRHHLHQQHLGAQKEQGPS*GNRSWSRRMSC*NSR*RSSRRTSRGSAVIVSA*MRRRKS *RSKWRSCRPRSPCQMPS*KHSKMRRRQEKPSDSRRGKQRPQESVTMWSPTQNISAGPTPTPTRPCQPWC HTMASRTGPRSATPLPPWPWSTRPHSPTRASSICRNTPGVYPVEGFEIQIRAPK*WTLPQPGLQNQSLQK MTVRGL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001258454
ORF Size	1908 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001258454.1, NP_001245383.1
RefSeq Size	2922 bp
RefSeq ORF	1911 bp
Locus ID	10318
UniProt ID	Q15025
MW	71.9 kDa
Gene Summary	This gene encodes an A20-binding protein which plays a role in autoimmunity and tissue homeostasis through the regulation of nuclear factor kappa-B activation. Mutations in this gene have been associated with psoriatic arthritis, rheumatoid arthritis, and systemic lupus erythematosus. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

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