FOXP1 (NM_001244816) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	12CC4; hFKH1B; HSPC215; MFH; QRF1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234698 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC RCATGATGCAAGAATCTGGGACTGAGACAAAAAGTAACGGTTCAGCCATCCAGAATGGGTCGGGCGGCAG CAACCACTTACTAGAGTGCGGCGGTCTTCGGGAGGGGCGGTCCAACGGAGAGACGCCGGCCGTGGACATC GGGGCAGCTGACCTCGCCCACGCCCAGCAGCAGCAGCAACAGGCACTTCAGGTGGCAAGACAGCTCCTTC TTCAGCAGCAACAGCAGCAGCAAGTTAGTGGATTAAAATCTCCCAAGAGGAATGACAAACAACCAGCTCT TCAGGTTCCCGTGTCAGTGGCTATGATGACACCTCAAGTTATCACTCCCCAGCAAATGCAGCAGATCCTC CAGCAACAAGTGCTGAGCCCTCAGCAGCTCCAGGTTCTCCTCCAGCAGCAGCAGGCCCTCATGCTTCAAC AGCAGCAGCTTCAAGAGTTTTATAAAAAACAACAGGAACAGTTGCAGCTTCAACTTTTACAACAACAACA TGCTGGAAAACAGCCTAAAGAGCAACAGCAGGTGGCTACCCAGCAGTTGGCTTTTCAGCAGCAGCTTTTA CAGATGCAGCAGTTACAGCAGCAGCACCTCCTGTCTTTGCAGCGCCAAGGCCTTCTGACAATTCAGCCCG GGCAGCCTGCCCTTCCCCTTCAACCTCTTGCTCAAGGCATGATTCCAACAGAACTGCAGCAGCTCTGGAA AGAAGTGACAAGTGCTCATACTGCAGAAGAAACCACAGGCAACAATCACAGCAGTTTGGATCTGACCACG ACATGTGTCTCCTCCTCTGCACCTTCCAAGACCTCCTTAATAATGAACCCACATGCCTCTACCAATGGAC AGCTCTCAGTCCACACTCCCAAAAGGGAAAGTTTGTCCCATGAGGAGCACCCCCATAGCCATCCTCTCTA TGGACATGGTGTATGCAAGTGGCCAGGCTGTGAAGCAGTGTGCGAAGATTTCCAATCATTTCTAAAACAT CTCAACAGTGAGCATGCGCTGGACGATAGAAGTACAGCCCAATGTAGAGTACAAATGCAGGTTGTACAGC AGTTAGAGCTACAGCTTGCAAAAGACAAAGAACGCCTGCAAGCCATGATGACCCACCTGCATGTGAAGTC TACAGAACCCAAAGCCGCCCCTCAGCCCTTGAATCTGGTATCAAGTGTCACTCTCTCCAAGTCCGCATCG GAGGCTTCTCCACAGAGCTTACCTCATACTCCAACGACCCCAACCGCCCCCCTGACTCCCGTCACCCAAG GCCCCTCTGTCATCACAACCACCAGCATGCACACGGTGGGACCCATCCGCAGGCGGTACTCAGACAAATA CAACGTGCCCATTTCGTCAGCAGATATTGCGCAGAACCAAGAATTTTATAAGAACGCAGAAGTTAGACCA CCATTTACATATGCATCTTTAATTAGGCAGGCCATTCTCGAATCTCCAGAAAAGCAGCTAACACTAAATG AGATCTATAACTGGTTCACACGAATGTTTGCTTACTTCCGACGCAACGCGGCCACGTGGAAGAATGCAGT GCGTCATAATCTTAGTCTTCACAAGTGTTTTGTGCGAGTAGAAAACGTTAAAGGGGCAGTATGGACAGTG GATGAAGTAGAATTCCAAAAACGAAGGCCACAAAAGATCAGTGGTAACCCTTCCCTTATTAAAAACATGC AGAGCAGCCACGCCTACTGCACACCTCTCAGTGCAGCTTTACAGGCTTCAATGGCTGAGAATAGTATACC TCTATACACTACCGCTTCCATGGGAAATCCCACTCTGGGCAACTTAGCCAGCGCAATACGGGAAGAGCTG AACGGGGCAATGGAGCATACCAACAGCAACGAGAGTGACAGCAGTCCAGGCAGATCTCCTATGCAAGCCG TGCATCCTGTACACGTCAAAGAAGAGCCCCTCGATCCAGAGGAAGCTGAAGGGCCCCTGTCCTTAGTGAC AACAGCCAACCACAGTCCAGATTTTGACCATGACAGAGATTACGAAGATGAACCAGTAAACGAGGACATG GAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234698 protein sequence Red=Cloning site Green=Tags(s) X*CKNLGLRQKVTVQPSRMGRAAATTY*SAAVFGRGGPTERRRPWTSGQLTSPTPSSSSNRHFRWQDSSF FSSNSSSKLVD*NLPRGMTNNQLFRFPCQWL**HLKLSLPSKCSRSSSNKC*ALSSSRFSSSSSRPSCFN SSSFKSFIKNNRNSCSFNFYNNNMLENSLKSNSRWLPSSWLFSSSFYRCSSYSSSTSCLCSAKAF*QFSP GSLPFPFNLLLKA*FQQNCSSSGKK*QVLILQKKPQATITAVWI*PRHVSPPLHLPRPP***THMPLPMD SSQSTLPKGKVCPMRSTPIAILSMDMVYASGQAVKQCAKISNHF*NISTVSMRWTIEVQPNVEYKCRLYS S*SYSLQKTKNACKP**PTCM*SLQNPKPPLSP*IWYQVSLSPSPHRRLLHRAYLILQRPQPPP*LPSPK APLSSQPPACTRWDPSAGGTQTNTTCPFRQQILRRTKNFIRTQKLDHHLHMHL*LGRPFSNLQKSS*H*M RSITGSHECLLTSDATRPRGRMQCVIILVFTSVLCE*KTLKGQYGQWMK*NSKNEGHKRSVVTLPLLKTC RAATPTAHLSVQLYRLQWLRIVYLYTLPLPWEIPLWAT*PAQYGKS*TGQWSIPTATRVTAVQADLLCKP CILYTSKKSPSIQRKLKGPCP**QQPTTVQILTMTEITKMNQ*TRTW TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001244816
ORF Size	2031 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001244816.1, NP_001231745.1
RefSeq Size	7007 bp
RefSeq ORF	2034 bp
Locus ID	27086
UniProt ID	Q9H334
Protein Families	Transcription Factors
MW	75.3 kDa
Gene Summary	This gene belongs to subfamily P of the forkhead box (FOX) transcription factor family. Forkhead box transcription factors play important roles in the regulation of tissue- and cell type-specific gene transcription during both development and adulthood. Forkhead box P1 protein contains both DNA-binding- and protein-protein binding-domains. This gene may act as a tumor suppressor as it is lost in several tumor types and maps to a chromosomal region (3p14.1) reported to contain a tumor suppressor gene(s). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

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