Treacher Collins syndrome protein (TCOF1) (NM_001195141) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	MFD1; TCS; TCS1; treacle
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235305 representing NM_001195141 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGAGGCCAGGAAGCGGCGGGAGCTACTTCCCCTGATCTACCACCATCTGCTGCGGGCTGGCTATG TGCGTGCGGCGCGGGAAGTGAAGGAGCAGAGCGGCCAGAAGTGTTTCCTGGCTCAGCCCGTAACCCTTCT GGACATCTATACACACTGGCAACAAACCTCAGAGCTTGGTCGGAAGCGGAAGGCAGAGGAAGATGCGGCA CTGCAAGCTAAGAAAACCCGTGTGTCAGACCCCATCAGCACCTCGGAGAGCTCGGAAGAGGAGGAAGAAG CAGAAGCCGAAACCGCCAAAGCCACCCCAAGACTAGCATCTACCAACTCCTCAGTCCTGGGGGCGGACTT GCCATCAAGCATGAAAGAAAAAGCCAAGGCAGAGACAGAGAAAGCTGGCAAGACTGGGAATTCCATGCCA CACCCTGCCACTGGGAAGACGGTGGCCAACCTTCTTTCTGGGAAGTCTCCCAGGAAGTCAGCAGAGCCCT CAGCAAATACTACGTTGGTCTCAGAAACTGAGGAGGAGGGCAGCGTCCCGGCCTTTGGAGCTGCTGCCAA GCCTGGGATGGTGTCAGCGGGCCAGGCCGACAGCTCCAGCGAGGACACCTCCAGCTCCAGTGATGAGACA GACGTGGAGGGGAAACCCTCAGTAAAACCAGCCCAGGTCAAAGCCTCATCAGTTTCTACTAAGGAGTCTC CAGCAAGAAAGGCGGCCCCAGCCCCTGGGAAGGTGGGGGATGTGACACCCCAGGTCAAAGGAGGGGCCCT GCCCCCAGCCAAGAGGGCCAAGAAGCCAGAAGAGGAGTCAGAGAGTAGTGAGGAGGGATCTGAAAGTGAG GAGGAGGCCCCTGCAGGGACACGAAGCCAGGTAAAGGCCTCTGAAAAAATTCTCCAGGTCAGAGCTGCCT CAGCCCCTGCCAAGGGGACCCCTGGGAAAGGGGCTACCCCAGCACCCCCTGGGAAGGCAGGGGCTGTAGC CTCCCAGACCAAGGCAGGGAAGCCAGAGGAGGACTCAGAGAGCAGCAGCGAGGAGTCATCTGACAGTGAG GAGGAGACGCCAGCTGCCAAGGCCCTGCTTCAGGCGAAGGCCTCAGGAAAAACCTCTCAGGTCGGAGCTG CCTCAGCCCCTGCCAAGGAGTCCCCCAGGAAAGGAGCTGCCCCAGCGCCCCCTGGGAAGACAGGGCCTGC AGTTGCCAAGGCCCAGGCGGGGAAGCGGGAGGAGGACTCGCAGAGCAGCAGCGAGGAATCGGACAGTGAG GAGGAGGCGCCTGCTCAGGCGAAGCCTTCAGGGAAGGCCCCCCAGGTCAGAGCCGCCTCGGCCCCTGCCA AGGAGTCCCCCAGGAAAGGGGCTGCCCCAGCACCTCCTAGGAAAACAGGGCCTGCAGCCGCCCAGGTCCA GGTGGGGAAGCAGGAGGAGGACTCAAGAAGCAGCAGCGAGGAGTCAGACAGTGACAGAGAGGCACTGGCA GCCATGAATGCAGCTCAGGTGAAGCCCTTGGGGAAAAGCCCCCAGGTGAAACCTGCCTCTACCATGGGCA TGGGGCCCTTGGGGAAAGGCGCCGGCCCAGTGCCACCCGGGAAGGTGGGGCCTGCAACCCCCTCAGCCCA GGTGGGGAAGTGGGAGGAGGACTCAGAGAGCAGTAGTGAGGAGTCATCAGACAGCAGTGATGGAGAGGTG CCCACAGCTGTGGCCCCGGCTCAGGAAAAGTCCTTGGGGAACATCCTCCAGGCCAAACCCACCTCCAGTC CTGCCAAGGGGCCCCCTCAGAAGGCAGGGCCTGTAGCCGTCCAGGTCAAGGCTGAAAAGCCCATGGACAA CTCGGAGAGCAGCGAGGAGTCATCGGACAGTGCGGACAGTGAGGAGGCACCAGCAGCCATGACTGCAGCT CAGGCAAAACCAGCTCTGAAAATTCCTCAGACCAAGGCCTGCCCAAAGAAAACCAATACCACTGCATCTG CCAAGGTCGCCCCTGTGCGAGTGGGCACCCAAGCCCCCCGGAAAGCAGGAACTGCGACTTCTCCAGCAGG CTCATCCCCAGCTGTGGCTGGGGGCACCCAGAGACCAGCAGAGGATTCTTCAAGCAGTGAGGAATCAGAT AGTGAGGAAGAGAAGACAGGTCTTGCAGTAACCGTGGGACAGGCAAAGTCTGTGGGGAAAGGCCTCCAGG TGAAAGCAGCCTCAGTGCCTGTCAAGGGGTCCTTGGGGCAAGGGACTGCTCCAGTACTCCCTGGGAAGAC GGGGCCTACAGTCACCCAGGTGAAAGCTGAAAAGCAGGAAGACTCTGAGAGCAGTGAGGAGGAATCAGAC AGTGAGGAAGCAGCTGCATCTCCAGCACAGGTGAAAACCTCAGTAAAGAAAACCCAGGCCAAAGCCAACC CAGCTGCCGCCAGAGCACCTTCAGCAAAAGGGACAATTTCAGCCCCTGGAAAAGTTGTCACTGCAGCTGC TCAAGCCAAGCAGAGGTCTCCATCCAAGGTGAAGCCACCAGTGAGAAACCCCCAGAACAGTACCGTCTTG GCGAGGGGCCCAGCATCTGTGCCATCTGTGGGGAAGGCCGTGGCTACAGCAGCTCAGGCCCAGACAGGGC CAGAGGAGGACTCAGGGAGCAGTGAGGAGGAGTCAGACAGTGAGGAGGAGGCGGAGACGCTGGCTCAGGT GAAGCCTTCAGGGAAGACCCACCAGATCAGAGCTGCCTTGGCTCCTGCCAAGGAGTCCCCCAGGAAAGGG GCTGCCCCAACACCTCCTGGGAAGACAGGGCCTTCGGCTGCCCAGGCAGGGAAGCAGGATGACTCAGGGA GCAGCAGCGAGGAATCAGACAGTGATGGGGAGGCACCGGCAGCTGTGACCTCTGCCCAGGTGATTAAACC CCCTCTGATTTTTGTCGACCCTAATCGTAGTCCAGCTGGCCCAGCTGCTACACCCGCACAAGCCCAGGCT GCAAGCACCCCGAGGAAGGCCCGAGCCTCGGAGAGCACAGCCAGGAGCTCCTCCTCCGAGAGCGAGGATG AGGACGTGATCCCCGCTACACAGTGCTTGACTCCTGGCATCAGAACCAATGTGGTGACCATGCCCACTGC CCACCCAAGAATAGCCCCCAAAGCCAGCATGGCTGGGGCCAGCAGCAGCAAGGAGTCCAGTCGGATATCA GATGGCAAGAAACAGGAGGGACCAGCCACTCAGGTTGACAGTGCTGTGGGAACACTCCCTGCAACAAGTC CCCAGAGCACCTCCGTCCAGGCCAAAGGGACCAACAAGCTCAGAAAACCTAAGCTTCCTGAGGTCCAGCA GGCCACCAAAGCCCCTGAGAGCTCAGATGACAGTGAGGACAGCAGCGACAGTTCTTCAGGGAGTGAGGAA GATGGTGAAGGGCCCCAGGGGGCCAAGTCAGCCCACACGCTGGGTCCCACCCCCTCCAGGACAGAGACCC TGGTGGAGGAGACCGCAGCAGAGTCCAGCGAGGATGATGTGGTGGCGCCATCCCAGTCTCTCCTCTCAGG TTATATGACCCCTGGACTAACCCCAGCCAATTCCCAGGCCTCAAAAGCCACTCCCAAGCTAGACTCCAGC CCCTCAGTTTCCTCTACTCTGGCCGCCAAAGATGACCCAGATGGCAAGCAGGAGGCAAAGCCCCAACAGG CAGCAGGCATGTTGTCCCCTAAAACAGGTGGAAAAGAGGCTGCTTCAGGCACCACACCTCAGAAGTCCCG GAAGCCCAAGAAAGGGGCTGGGAACCCCCAAGCCTCAACCCTGGCGCTGCAAAGCAACATCACCCAGTGC CTCCTGGGCCAACCCTGGCCCCTGAATGAGGCCCAGGTGCAGGCCTCAGTGGTGAAGGTCCTGACTGAGC TGCTGGAACAGGAAAGAAAGAAGGTGGTGGACACCACCAAGGAGAGCAGCAGGAAGGGCTGGGAGAGCCG CAAGCGGAAGCTATCGGGAGACCAGCCAGCTGCCAGGACCCCCAGGAGCAAGAAGAAGAAGAAGCTGGGG GCCGGGGAAGGTGGGGAGGCCTCTGTTTCCCCAGAAAAGACCTCCACGACTTCCAAGGGGAAAGCAAAGA GAGACAAAGCAAGTGGTGATGTCAAGGAGAAGAAAGGGAAGGGGTCTCTTGGCTCCCAAGGGGCCAAGGA CGAGCCAGAAGAGGAGCTTCAGAAGGGGATGGGGACGGTTGAAGGTGGAGATCAAAGCAACCCAAAGAGC AAGAAGGAGAAGAAGAAATCCGACAAGAGAAAAAAAGACAAAGAAAAAAAAGAAAAGAAGAAGAAAGCAA AAAAGGCCTCAACCAAAGATTCTGAGTCACCGTCCCAGAAGAAAAAGAAGAAAAAGAAGAAGACAGCAGA GCAGACTGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC235305 representing NM_001195141 Red=Cloning site Green=Tags(s) MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAA LQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMP HPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDET DVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKRAKKPEEESESSEEGSESE EEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSE EETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSE EEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALA AMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEV PTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAA QAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESD SEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESD SEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPPVRNPQNSTVL ARGPASVPSVGKAVATAAQAQTGPEEDSGSSEEESDSEEEAETLAQVKPSGKTHQIRAALAPAKESPRKG AAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQVIKPPLIFVDPNRSPAGPAATPAQAQA ASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRIS DGKKQEGPATQVDSAVGTLPATSPQSTSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEE DGEGPQGAKSAHTLGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSS PSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNITQC LLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQPAARTPRSKKKKKLG AGEGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGTVEGGDQSNPKS KKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001195141
ORF Size	4350 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001195141.2
RefSeq Size	4999 bp
RefSeq ORF	4353 bp
Locus ID	6949
UniProt ID	Q13428
Protein Families	Druggable Genome, Stem cell - Pluripotency
MW	148.7 kDa
Gene Summary	This gene encodes a nucleolar protein with a LIS1 homology domain. The protein is involved in ribosomal DNA gene transcription through its interaction with upstream binding factor (UBF). Mutations in this gene have been associated with Treacher Collins syndrome, a disorder which includes abnormal craniofacial development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

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