ZNF274 (NM_001278734) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	HFB101; ZF2; ZKSCAN19; ZSCAN51
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239079 representing NM_001278734 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGAGAACTACAGGAACCTGGTCTCAGTGGAACATCAGCTTTCCAAACCAGATGTGGTATCTCAGT TAGAGGAGGCAGAAGATTTCTGGCCAGTGGAGAGAGGAATTCCTCAAGACACCATTCCAGAGTATCCTGA GCTCCAGCTGGACCCTAAATTGGATCCTCTTCCTGCTGAGAGTCCCCTAATGAACATTGAGGTTGTTGAG GTCCTCACACTGAACCAGGAGGTGGCTGGTCCCCGGAATGCCCAGATCCAGGCCCTATATGCTGAAGATG GAAGCCTGAGTGCAGATGCCCCCAGTGAGCAGGTCCAACAGCAGGGCAAGCATCCAGGTGACCCTGAGGC CGCGCGCCAGAGGTTCCGGCAGTTCCGTTATAAGGACATGACAGGTCCCCGGGAGGCCCTGGACCAGCTC CGAGAGCTGTGTCACCAGTGGCTACAGCCTAAGGCACGCTCCAAGGAGCAGATCCTGGAGCTGCTGGTGC TGGAGCAGTTCCTAGGTGCACTGCCTGTGAAGCTCCGGACATGGGTGGAATCGCAGCACCCAGAGAACTG CCAAGAGGTGGTGGCCCTGGTAGAGGGTGTGACCTGGATGTCTGAGGAGGAAGTACTTCCTGCAGGACAA CCTGCCGAGGGCACCACCTGCTGCCTCGAGGTCACTGCCCAGCAGGAGGAGAAGCAGGAGGATGCAGCCA TCTGCCCAGTGACAGTGCTCCCTGAGGAGCCAGTGACCTTCCAGGATGTGGCTGTGGACTTCAGCCGGGA GGAGTGGGGGCTGCTGGGCCCGACACAGAGGACCGAGTACCGCGATGTGATGCTGGAGACCTTTGGGCAC CTGGTCTCTGTGGGGTGGGAGACTACACTGGAAAATAAAGAGTTAGCTCCAAATTCTGACATTCCTGAGG AAGAACCAGCCCCCAGCCTGAAAGTACAAGAATCCTCAAGGGATTGTGCCTTGTCCTCTACATTAGAAGA TACCTTGCAGGGTGGGGTCCAGGAAGTCCAAGACACAGTGTTGAAGCAGATGGAGTCTGCTCAGGAAAAA GACCTTCCTCAGAAGAAGCACTTTGACAACCGTGAGTCCCAGGCAAACAGTGGTGCTCTTGACACAAACC AAGTTTCGCTCCAGAAAATTGACAACCCTGAGTCCCAGGCAAACAGTGGCGCTCTTGACACAAACCAAGT TTTGCTCCACAAAATTCCTCCTAGAAAACGATTGCGCAAACGTGACTCACAAGTTAAAAGTATGAAACAT AATTCACGTGTAAAAATTCATCAGAAGAGCTGTGAAAGGCAAAAGGCCAAGGAAGGCAATGGTTGTAGGA AAACCTTCAGTCGGAGTACTAAACAGATTACGTTTATAAGAATTCACAAGGGGAGCCAAGTTTGCCGATG CAGTGAATGTGGTAAAATATTCCGGAACCCAAGATACTTTTCTGTGCATAAGAAAATCCATACCGGAGAG AGGCCCTATGTGTGTCAAGACTGTGGGAAAGGATTTGTTCAGAGCTCTTCCCTCACACAGCATCAGAGAG TTCATTCTGGAGAGAGACCATTTGAATGTCAGGAGTGTGGGAGGACCTTCAATGATCGCTCAGCCATCTC CCAGCACCTGAGGACTCACACTGGCGCTAAGCCCTACAAGTGTCAGGACTGTGGAAAAGCCTTCCGCCAG AGCTCCCACCTCATCAGACATCAGAGGACTCACACCGGGGAGCGCCCATATGCATGCAACAAATGTGGAA AGGCCTTCACCCAGAGCTCACACCTTATTGGGCACCAGAGAACCCACAATAGGACAAAGCGAAAGAAGAA ACAGCCTACCTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239079 representing NM_001278734 Red=Cloning site Green=Tags(s) MLENYRNLVSVEHQLSKPDVVSQLEEAEDFWPVERGIPQDTIPEYPELQLDPKLDPLPAESPLMNIEVVE VLTLNQEVAGPRNAQIQALYAEDGSLSADAPSEQVQQQGKHPGDPEAARQRFRQFRYKDMTGPREALDQL RELCHQWLQPKARSKEQILELLVLEQFLGALPVKLRTWVESQHPENCQEVVALVEGVTWMSEEEVLPAGQ PAEGTTCCLEVTAQQEEKQEDAAICPVTVLPEEPVTFQDVAVDFSREEWGLLGPTQRTEYRDVMLETFGH LVSVGWETTLENKELAPNSDIPEEEPAPSLKVQESSRDCALSSTLEDTLQGGVQEVQDTVLKQMESAQEK DLPQKKHFDNRESQANSGALDTNQVSLQKIDNPESQANSGALDTNQVLLHKIPPRKRLRKRDSQVKSMKH NSRVKIHQKSCERQKAKEGNGCRKTFSRSTKQITFIRIHKGSQVCRCSECGKIFRNPRYFSVHKKIHTGE RPYVCQDCGKGFVQSSSLTQHQRVHSGERPFECQECGRTFNDRSAISQHLRTHTGAKPYKCQDCGKAFRQ SSHLIRHQRTHTGERPYACNKCGKAFTQSSHLIGHQRTHNRTKRKKKQPTS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001278734
ORF Size	1833 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278734.1, NP_001265663.1
RefSeq Size	2905 bp
RefSeq ORF	1836 bp
Locus ID	10782
UniProt ID	Q96GC6
Protein Families	Transcription Factors
Protein Pathways	Neurotrophin signaling pathway
MW	69.8 kDa
Gene Summary	This gene encodes a zinc finger protein containing five C2H2-type zinc finger domains, one or two Kruppel-associated box A (KRAB A) domains, and a leucine-rich domain. The encoded protein has been suggested to be a transcriptional repressor. It localizes predominantly to the nucleolus. Alternatively spliced transcript variants encoding different isoforms exist. These variants utilize alternative polyadenylation signals. [provided by RefSeq, Jul 2008]

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