CDC27 (NM_001293091) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ANAPC3; APC3; CDC27Hs; D0S1430E; D17S978E; H-NUC; HNUC; NUC2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239512 representing NM_001293091 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAGCACTAAACCACTATGCTTACCGAGATGCGGTTTTCCTCGCAGAACGCCTTTATGCAGAAGGC TTGCAGAAGGGGAACAAATCTTATCTGGTGGAGTGTTTAATAAGCAGAAAAGCCATGATGATATTGTTAC TGAGTTTGGTGATTCAGCTTGCTTTACTCTTTCATTGTTGGGACATGTATATTGCAAGACAGATCGGCTT GCCAAAGGATCAGAATGTTACCAAAAGAGCCTTAGTTTAAATCCTTTCCTCTGGTCTCCCTTTGAATCAT TATGTGAAATAGGTGAAAAGCCAGATCCTGACCAAACATTTAAATTCACATCTTTACAGAACTTTAGCAA CTGTCTGCCCAACTCTTGCACAACACAAGTACCTAATCATAGTTTATCTCACAGACAGCCTGAGACAGTT CTTACGGAAACACCCCAGGACACAATTGAATTAAACAGATTGAATTTAGAATCTTCCAATTCAAAGTACT CCTTGAATACAGATTCCTCAGTGTCTTATATTGATTCAGCTGTAATTTCACCTGATACTGTCCCACTGGG AACAGGAACTTCCATATTATCTAAACAGGTTCAAAATAAACCAAAAACTGGTCGAAGTTTATTAGGAGGA CCAGCAGCTCTTAGTCCATTAACCCCAAGTTTTGGGATTTTGCCATTAGAAACCCCAAGTCCTGGAGATG GATCCTATTTACAAAACTACACTAATACACCTCCTGTAATTGATGTGCCATCCACCGGAGCCCCTTCAAA AAAGTCTGTTGCCAGAATCGGCCAAACTGGAACAAAGTCTGTCTTCTCACAGAGTGGAAATAGCCGAGAG GTAACTCCAATTCTTGCACAAACACAAAGTTCTGGTCCACAAACAAGTACAACACCTCAGGTATTGAGCC CCACTATTACATCTCCCCCAAACGCACTGCCTCGAAGAAGTTCACGACTCTTTACTAGTGACAGCTCCAC AACCAAGGAGAATAGCAAAAAATTAAAAATGAAGTTTCCACCTAAAATCCCAAACAGAAAAACAAAAAGT AAAACTAATAAAGGAGGAATAACTCAACCTAACATAAATGATAGCCTGGAAATTACAAAATTGGACTCTT CCATCATTTCAGAAGGGAAAATATCCACAATCACACCTCAGATTCAGGCCTTTAATCTACAAAAAGCAGC AGCAGAAGGTTTGATGAGCCTTCTTCGTGAAATGGGGAAAGGTTATTTAGCTTTGTGTTCATACAACTGC AAAGAAGCTATAAATATTTTGAGCCATCTACCTTCTCACCACTACAATACTGGTTGGGTACTGTGCCAAA TTGGAAGGGCCTATTTTGAACTTTCAGAGTACATGCAAGCTGAAAGAATATTCTCAGAGGTTAGAAGGAT TGAGAATTATAGAGTTGAAGGCATGGAGATCTACTCTACAACACTTTGGCATCTTCAAAAAGATGTTGCT CTTTCAGTTCTGTCAAAAGACTTAACAGACATGGATAAAAATTCGCCAGAGGCCTGGTGTGCTGCAGGGA ACTGTTTCAGTCTGCAACGGGAACATGATATTGCAATTAAATTCTTCCAGAGAGCTATCCAAGTTGATCC AAATTACGCTTATGCCTATACTCTATTAGGGCATGAGTTTGTCTTAACTGAAGAATTGGACAAAGCATTA GCTTGTTTTCGAAATGCTATCAGAGTCAATCCTAGACATTATAATGCATGGTATGGTTTAGGAATGATTT ATTACAAGCAAGAAAAATTCAGCCTTGCAGAAATGCATTTCCAAAAAGCGCTTGATATCAACCCTCAAAG TTCAGTTTTACTTTGCCACATTGGAGTAGTTCAACATGCACTGAAAAAATCAGAGAAGGCTTTGGATACC CTAAACAAAGCCATTGTCATTGATCCCAAGAACCCTCTATGCAAATTTCACAGAGCCTCAGTTTTATTTG CAAATGAAAAATATAAGTCTGCTTTACAAGAACTTGAAGAATTGAAACAAATTGTTCCCAAAGAATCCCT CGTTTACTTCTTAATAGGAAAGGTTTACAAGAAGTTAGGTCAAACGCACCTCGCCCTGATGAATTTCTCT TGGGCTATGGATTTAGATCCTAAAGGAGCCAATAACCAGATTAAAGAGGCAATTGATAAGCGTTATCTTC CAGATGATGAGGAGCCAATAACCCAAGAAGAACAGATCATGGGAACAGATGAATCCCAGGAGAGCAGCAT GACAGATGCGGATGACACACAACTTCATGCAGCTGAAAGTGATGAATTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239512 representing NM_001293091 Red=Cloning site Green=Tags(s) MASTKPLCLPRCGFPRRTPLCRRLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRL AKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETV LTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGG PAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKSVARIGQTGTKSVFSQSGNSRE VTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKS KTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAEGLMSLLREMGKGYLALCSYNC KEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVA LSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKAL ACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDT LNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFS WAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001293091
ORF Size	2289 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001293091.3
RefSeq Size	5705 bp
RefSeq ORF	2292 bp
Locus ID	996
Protein Families	Druggable Genome
Protein Pathways	Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis
MW	85.3 kDa
Gene Summary	The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 22 and Y. [provided by RefSeq, May 2014]

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