ITGA8 (NM_001291494) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239915 representing NM_001291494 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGCCCGGGGCCAGCCGCGGTCCCCGGGGAAGCCAGGCGCCGCTGATCGCGCCCCTCTGCTGCGCCG CGGCCGCGCTGGGGATGTTGCTGTGGTCCCCCGCCTGTCAGGCGTTCAACCTGGACGTGGAAAAGCTCAC AGTGTACAGCGGCCCCAAGGGCAGCTACTTCGGCTACGCCGTGGACTTCCACATACCCGACGCCCGCACA GCGAGTGTCTTGGTGGGGGCGCCCAAAGCCAACACCAGCCAGCCCGATATCGTGGAAGGGGGAGCCGTCT ATTACTGTCCTTGGCCCGCGGAGGGGTCTGCGCAGTGCAGGCAGATACCGTTTGACACCACCAACAACAG AAAGATCAGAGTTAATGGAACCAAAGAACCTATCGAGTTCAAATCCAATCAGTGGTTTGGAGCAACAGTG AAAGCTCACAAAGGAAAAGTTGTGGCCTGTGCTCCTTTATATCACTGGAGAACTCTTAAACCGACACCAG AAAAGGACCCAGTTGGCACCTGCTATGTAGCAATTCAGAACTTCAGCGCCTATGCCGAGTTCTCTCCTTG CCGGAACAGCAATGCTGATCCGGAAGGCCAGGGTTACTGCCAAGCAGGATTTAGTCTGGATTTTTATAAG AATGGAGACCTTATTGTGGGAGGACCTGGGAGTTTCTACTGGCAAGGACAAGTGATCACTGCCAGTGTTG CAGATATCATTGCAAATTACTCATTCAAGGATATCCTCAGGAAACTGGCAGGAGAAAAGCAGACGGAAGT GGCTCCAGCTTCCTATGATGACAGTTACCTTGAATTGGTTGCTGGAATTCCAAGAGGAGCACAGAATTTT GGATATGTTTCCATCATTAACTCTACGGATATGACGTTTATTCAGAATTTCACGGGAGAACAGATGGCAT CTTATTTTGGATATACCGTTGTCGTATCAGATGTTAACAGTGATGGACTGGATGATGTCCTGGTTGGGGC ACCTCTCTTTATGGAACGTGAATTTGAGAGCAACCCCAGAGAAGTAGGGCAAATCTACCTGTATTTGCAA GTGAGCTCTCTCCTCTTCAGAGACCCCCAGATCCTCACTGGCACCGAGACGTTTGGGAGATTCGGTAGTG CTATGGCACACTTAGGAGACCTGAACCAAGATGGATACAATGACATTGCCATCGGAGTGCCTTTTGCAGG CAAGGATCAAAGAGGCAAAGTGCTCATTTATAATGGGAACAAAGATGGCTTAAACACCAAGCCTTCCCAA GTTCTGCAAGGAGTGTGGGCCTCACATGCTGTCCCTTCCGGATTTGGCTTTACTTTAAGAGGAGATTCAG ACATAGACAAGAATGATTACCCAGATTTGATTGTGGGTGCATTTGGAACAGGAAAAGTCGCTGTTTACAG AGCAAGACCGGTTGTGACTGTAGATGCCCAGCTTCTGCTGCACCCAATGATTATCAATCTTGAAAATAAA ACTTGCCAGGTTCCAGACTCTATGACATCTGCTGCCTGCTTTTCTTTAAGAGTATGTGCATCTGTCACAG GCCAGAGCATTGCAAACACAATAGTCTTGATGGCAGAGGTGCAATTAGATTCCCTGAAACAGAAAGGAGC TATTAAACGGACGCTCTTCCTTGATAACCATCAGGCTCATCGCGTCTTCCCTCTTGTGATAAAAAGGCAG AAATCCCACCAGTGCCAGGATTTCATCGTTTACCTTCGAGATGAAACTGAATTCCGAGATAAATTATCTC CAATCAACATTAGTTTGAATTACAGTTTGGACGAATCCACCTTTAAAGAAGGCCTGGAAGTGAAACCAAT ATTGAACTACTACAGAGAAAACATTGTTAGTGAACAGGCTCACATTCTGGTGGACTGTGGAGAAGACAAT CTGTGTGTTCCTGACTTGAAGCTGTCGGCTAGACCAGATAAGCATCAGGTAATCATTGGAGATGAAAATC ACCTTATGCTCATAATAAATGCAAGAAATGAAGGGGAAGGAGCATATGAAGCTGAACTCTTTGTAATGAT ACCAGAAGAGGCAGATTATGTTGGAATCGAACGCAACAACAAGGGATTTCGACCACTGAGCTGTGAGTAC AAGATGGAAAATGTAACCAGGATGGTGGTGTGTGACCTTGGGAACCCTATGGTGTCTGGAACAAATTATT CCCTGGGCCTCCGATTTGCAGTTCCACGTCTTGAGAAAACAAACATGAGCATTAACTTCGATCTCCAAAT CAGAAGTTCCAACAAGGACAATCCAGACAGCAATTTTGTGAGCCTGCAAATCAACATCACTGCTGTAGCG CAGGTGGAAATAAGAGGAGTGTCACACCCTCCGCAGATTGTTCTGCCCATTCATAACTGGGAACCAGAAG AGGAGCCCCACAAAGAGGAGGAGGTTGGACCATTGGTGGAACATATTTATGAGCTGCACAATATTGGACC AAGTACCATCAGTGACACCATCCTGGAGGTGGGCTGGCCTTTCTCTGCCCGGGATGAATTTCTTCTCTAT ATTTTCCATATTCAAACTCTGGGACCTCTGCAGTGCCAACCAAATCCTAATATCAATCCACAGGATATAA AGCCTGCTGCCTCCCCAGAGGACACCCCTGAGCTCAGCGCCTTTTTGCGAAACTCTACTATTCCTCATCT TGTCAGGAAGAGGGATGTACATGTGGTCGAATTCCACAGACAGAGCCCTGCAAAAATACTGAATTGTACA AATATCGAGTGTTTACAAATCTCCTGTGCAGTGGGACGACTCGAAGGAGGAGAAAGCGCAGTCCTGAAAG TCAGGTCACGATTATGGGCCCACACCTTCCTCCAGAGAAAAAATGATCCCTATGCTCTTGCATCCCTGGT GTCCTTTGAAGTTAAGAAGATGCCTTATACAGATCAGCCAGCAAAACTCCCAGAAGGAAGCATAGTAATT AAGACATCAGTTATTTGGGCAACTCCGAATGTTTCCTTCTCAATCCCATTATGGGTAATAATACTAGCAA TACTTCTTGGATTGTTGGTTCTCGCCATTTTAACCTTAGCTTTATGGAAGTGTGGATTCTTTGACAGAGC CAGACCTCCTCAGGAGGACATGACCGACAGGGAACAGCTGACAAATGACAAGACCCCTGAGGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239915 representing NM_001291494 Red=Cloning site Green=Tags(s) MSPGASRGPRGSQAPLIAPLCCAAAALGMLLWSPACQAFNLDVEKLTVYSGPKGSYFGYAVDFHIPDART ASVLVGAPKANTSQPDIVEGGAVYYCPWPAEGSAQCRQIPFDTTNNRKIRVNGTKEPIEFKSNQWFGATV KAHKGKVVACAPLYHWRTLKPTPEKDPVGTCYVAIQNFSAYAEFSPCRNSNADPEGQGYCQAGFSLDFYK NGDLIVGGPGSFYWQGQVITASVADIIANYSFKDILRKLAGEKQTEVAPASYDDSYLELVAGIPRGAQNF GYVSIINSTDMTFIQNFTGEQMASYFGYTVVVSDVNSDGLDDVLVGAPLFMEREFESNPREVGQIYLYLQ VSSLLFRDPQILTGTETFGRFGSAMAHLGDLNQDGYNDIAIGVPFAGKDQRGKVLIYNGNKDGLNTKPSQ VLQGVWASHAVPSGFGFTLRGDSDIDKNDYPDLIVGAFGTGKVAVYRARPVVTVDAQLLLHPMIINLENK TCQVPDSMTSAACFSLRVCASVTGQSIANTIVLMAEVQLDSLKQKGAIKRTLFLDNHQAHRVFPLVIKRQ KSHQCQDFIVYLRDETEFRDKLSPINISLNYSLDESTFKEGLEVKPILNYYRENIVSEQAHILVDCGEDN LCVPDLKLSARPDKHQVIIGDENHLMLIINARNEGEGAYEAELFVMIPEEADYVGIERNNKGFRPLSCEY KMENVTRMVVCDLGNPMVSGTNYSLGLRFAVPRLEKTNMSINFDLQIRSSNKDNPDSNFVSLQINITAVA QVEIRGVSHPPQIVLPIHNWEPEEEPHKEEEVGPLVEHIYELHNIGPSTISDTILEVGWPFSARDEFLLY IFHIQTLGPLQCQPNPNINPQDIKPAASPEDTPELSAFLRNSTIPHLVRKRDVHVVEFHRQSPAKILNCT NIECLQISCAVGRLEGGESAVLKVRSRLWAHTFLQRKNDPYALASLVSFEVKKMPYTDQPAKLPEGSIVI KTSVIWATPNVSFSIPLWVIILAILLGLLVLAILTLALWKCGFFDRARPPQEDMTDREQLTNDKTPEA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001291494
ORF Size	3144 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291494.1, NP_001278423.1
RefSeq Size	6937 bp
RefSeq ORF	3147 bp
Locus ID	8516
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Arrhythmogenic right ventricular cardiomyopathy (ARVC), Cell adhesion molecules (CAMs), Dilated cardiomyopathy, ECM-receptor interaction, Focal adhesion, Hypertrophic cardiomyopathy (HCM), Regulation of actin cytoskeleton
MW	116.4 kDa
Gene Summary	Integrins are heterodimeric transmembrane receptor proteins that mediate numerous cellular processes including cell adhesion, cytoskeletal rearrangement, and activation of cell signaling pathways. Integrins are composed of alpha and beta subunits. This gene encodes the alpha 8 subunit of the heterodimeric integrin alpha8beta1 protein. The encoded protein is a single-pass type 1 membrane protein that contains multiple FG-GAP repeats. This repeat is predicted to fold into a beta propeller structure. This gene regulates the recruitment of mesenchymal cells into epithelial structures, mediates cell-cell interactions, and regulates neurite outgrowth of sensory and motor neurons. The integrin alpha8beta1 protein thus plays an important role in wound-healing and organogenesis. Mutations in this gene have been associated with renal hypodysplasia/aplasia-1 (RHDA1) and with several animal models of chronic kidney disease. Alternate splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Apr 2014]

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