RGS3 (NM_001282923) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	C2PA; RGP3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239957 representing NM_001282923 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCAGGACCGGGTTCTGCTGCTTCACAGTCTCTTTATTCGTGTGGATGGATATGTCTATGTGTGTCT CTCTTTCTCGCTGTGTGTGTGTGTATGTTTCCATTCATCCACCCCAATGTCTGAATTCTCTTTTAGTTAT AGAAGGTAAAGGCCTGATCAGCAAACAGCCTGGCACCTGTGATCCGTATGTGAAGATTTCTTTGATCCCT GAAGATAGTAGACTACGCCACCAGAAGACGCAGACCGTTCCAGACTGCAGAGACCCGGCTTTCCACGAGC ACTTCTTCTTTCCTGTCCAAGAGGAGGATGATCAGAAGCGTCTCTTGGTTACTGTGTGGAACAGGGCCAG CCAGTCCAGACAGAGTGGACTCATTGGCTGCATGAGCTTTGGGGTGAAGTCTCTCCTGACTCCAGACAAG GAGATCAGTGGTTGGTACTACCTCCTAGGGGAGCACCTGGGCCGGACCAAGCACTTGAAGGTGGCCAGGC GGCGACTGCGGCCGCTGAGAGACCCGCTGCTGAGAATGCCAGGAGGTGGGGACACTGAGAATGGGAAGAA ACTAAAGATCACCATCCCGAGGGGAAAGGACGGCTTTGGCTTCACCATCTGCTGCGACTCTCCAGTTCGA GTCCAGGCCGTGGATTCCGGGGGTCCGGCGGAACGGGCAGGGCTGCAGCAGCTGGACACGGTGCTGCAGC TGAATGAGAGGCCTGTGGAGCACTGGAAATGTGTGGAGCTGGCCCACGAGATCCGGAGCTGCCCCAGTGA GATCATCCTACTCGTGTGGCGCATGGTCCCCCAGGTCAAGCCAGGACCAGATGGCGGGGTCCTGCGGCGG GCCTCCTGCAAGTCGACACATGACCTCCAGTCACCCCCCAACAAACGGGAGAAGAACTGCACCCATGGGG TCCAGGCACGGCCTGAGCAGCGCCACAGCTGCCACCTGGTATGTGACAGCTCTGATGGGCTGCTGCTCGG CGGCTGGGAGCGCTACACCGAGGTGGCCAAGCGCGGGGGCCAGCACACCCTGCCTGCACTGTCCCGTGCC ACTGCCCCCACCGACCCCAACTACATCATCCTGGCCCCGCTGAATCCTGGGAGCCAGCTGCTCCGGCCTG TGTACCAGGAGGATACCATCCCCGAAGAATCAGGGAGTCCCAGTAAAGGGAAGTCCTACACAGGCCTGGG GAAGAAGTCCCGGCTGATGAAGACAGTGCAGACCATGAAGGGCCACGGGAACTACCAAAACTGCCCGGTT GTGAGGCCGCATGCCACGCACTCAAGCTATGGCACCTACGTCACCCTGGCCCCCAAAGTCCTGGTGTTCC CTGTCTTTGTTCAGCCTCTAGATCTCTGTAATCCTGCCCGGACCCTCCTGCTGTCAGAGGAGCTGCTGCT GTATGAAGGGAGGAACAAGGCTGCCGAGGTGACACTGTTTGCCTATTCGGACCTGCTGCTCTTCACCAAG GAGGACGAGCCTGGCCGCTGCGACGTCCTGAGGAACCCCCTCTACCTCCAGAGTGTGAAGCTGCAGGAAG GTTCTTCAGAAGACCTGAAATTCTGCGTGCTCTATCTAGCAGAGAAGGCAGAGTGCTTATTCACTTTGGA AGCGCACTCGCAGGAGCAGAAGAAGAGAGTGTGCTGGTGCCTGTCGGAGAACATCGCCAAGCAGCAACAG CTGGCAGCATCACCCCCGGACAGCAAGATGTTTGAGACGGAGGCAGATGAGAAGAGGGAGATGGCCTTGG AGGAAGGGAAGGGGCCTGGTGCCGAGGATTCCCCACCCAGCAAGGAGCCCTCTCCTGGCCAGGAGCTTCC TCCAGGACAAGACCTTCCACCCAACAAGGACTCCCCTTCTGGGCAGGAACCCGCTCCCAGCCAAGAACCA CTGTCCAGCAAAGACTCAGCTACCTCTGAAGGATCCCCTCCAGGCCCAGATGCTCCGCCCAGCAAGGATG TGCCACCATGCCAGGAACCCCCTCCAGCCCAAGACCTCTCACCCTGCCAGGACCTACCTGCTGGTCAAGA ACCCCTGCCTCACCAGGACCCTCTACTCACCAAAGACCTCCCTGCCATCCAGGAATCCCCCACCCGGGAC CTTCCACCCTGTCAAGATCTGCCTCCTAGCCAGGTCTCCCTGCCAGCCAAGGCCCTTACTGAGGACACCA TGAGCTCCGGGGACCTACTAGCAGCTACTGGGGACCCACCTGCGGCCCCCAGGCCAGCCTTCGTGATCCC TGAGGTCCGGCTGGATAGCACCTACAGCCAGAAGGCAGGGGCAGAGCAGGGCTGCTCGGGAGATGAGGAG GATGCAGAAGAGGCCGAGGAGGTGGAGGAGGGGGAGGAAGGGGAGGAGGACGAGGATGAGGACACCAGCG ATGACAACTACGGAGAGCGCAGTGAGGCCAAGCGCAGCAGCATGATCGAGACGGGCCAGGGGGCTGAGGG TGGCCTCTCACTGCGTGTGCAGAACTCGCTGCGGCGCCGGACGCACAGCGAGGGCAGCCTGCTGCAGGAG CCCCGAGGGCCCTGCTTTGCCTCCGACACCACCTTGCACTGCTCAGACGGTGAGGGCGCCGCCTCCACCT GGGGCATGCCTTCGCCCAGCACCCTCAAGAAAGAGCTGGGCCGCAATGGTGGCTCCATGCACCACCTTTC CCTCTTCTTCACAGGACACAGGAAGATGAGCGGGGCTGACACCGTTGGGGATGATGACGAAGCCTCCCGG AAGAGAAAGAGCAAAAACCTAGCCAAGGACATGAAGAACAAGCTGGGGATCTTCAGACGGCGGAATGAGT CCCCTGGAGCCCCTCCCGCGGGCAAGGCAGACAAAATGATGAAGTCATTCAAGCCCACCTCAGAGGAAGC CCTCAAGTGGGGCGAGTCCTTGGAGAAGCTGCTGGTTCACAAATACGGGTTAGCAGTGTTCCAAGCCTTC CTTCGCACTGAGTTCAGTGAGGAGAATCTGGAGTTCTGGTTGGCTTGTGAGGACTTCAAGAAGGTCAAGT CACAGTCCAAGATGGCATCCAAGGCCAAGAAGATCTTTGCTGAATACATCGCGATCCAGGCATGCAAGGA GGTCAACCTGGACTCCTACACGCGGGAGCACACCAAGGACAACCTGCAGAGCGTCACGCGGGGCTGCTTC GACCTGGCACAGAAGCGCATCTTCGGGCTCATGGAAAAGGACTCGTACCCTCGCTTTCTCCGTTCTGACC TCTACCTGGACCTTATTAACCAGAAGAAGATGAGTCCCCCGCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239957 representing NM_001282923 Red=Cloning site Green=Tags(s) MPRTGFCCFTVSLFVWMDMSMCVSLSRCVCVYVSIHPPQCLNSLLVIEGKGLISKQPGTCDPYVKISLIP EDSRLRHQKTQTVPDCRDPAFHEHFFFPVQEEDDQKRLLVTVWNRASQSRQSGLIGCMSFGVKSLLTPDK EISGWYYLLGEHLGRTKHLKVARRRLRPLRDPLLRMPGGGDTENGKKLKITIPRGKDGFGFTICCDSPVR VQAVDSGGPAERAGLQQLDTVLQLNERPVEHWKCVELAHEIRSCPSEIILLVWRMVPQVKPGPDGGVLRR ASCKSTHDLQSPPNKREKNCTHGVQARPEQRHSCHLVCDSSDGLLLGGWERYTEVAKRGGQHTLPALSRA TAPTDPNYIILAPLNPGSQLLRPVYQEDTIPEESGSPSKGKSYTGLGKKSRLMKTVQTMKGHGNYQNCPV VRPHATHSSYGTYVTLAPKVLVFPVFVQPLDLCNPARTLLLSEELLLYEGRNKAAEVTLFAYSDLLLFTK EDEPGRCDVLRNPLYLQSVKLQEGSSEDLKFCVLYLAEKAECLFTLEAHSQEQKKRVCWCLSENIAKQQQ LAASPPDSKMFETEADEKREMALEEGKGPGAEDSPPSKEPSPGQELPPGQDLPPNKDSPSGQEPAPSQEP LSSKDSATSEGSPPGPDAPPSKDVPPCQEPPPAQDLSPCQDLPAGQEPLPHQDPLLTKDLPAIQESPTRD LPPCQDLPPSQVSLPAKALTEDTMSSGDLLAATGDPPAAPRPAFVIPEVRLDSTYSQKAGAEQGCSGDEE DAEEAEEVEEGEEGEEDEDEDTSDDNYGERSEAKRSSMIETGQGAEGGLSLRVQNSLRRRTHSEGSLLQE PRGPCFASDTTLHCSDGEGAASTWGMPSPSTLKKELGRNGGSMHHLSLFFTGHRKMSGADTVGDDDEASR KRKSKNLAKDMKNKLGIFRRRNESPGAPPAGKADKMMKSFKPTSEEALKWGESLEKLLVHKYGLAVFQAF LRTEFSEENLEFWLACEDFKKVKSQSKMASKAKKIFAEYIAIQACKEVNLDSYTREHTKDNLQSVTRGCF DLAQKRIFGLMEKDSYPRFLRSDLYLDLINQKKMSPPL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282923
ORF Size	3264 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282923.2
RefSeq Size	4279 bp
RefSeq ORF	3267 bp
Locus ID	5998
UniProt ID	P49796
Protein Families	Druggable Genome
Protein Pathways	Axon guidance
MW	120.7 kDa
Gene Summary	This gene encodes a member of the regulator of G-protein signaling (RGS) family. This protein is a GTPase-activating protein that inhibits G-protein-mediated signal transduction. Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different isoforms. Long isoforms are largely cytosolic and plasma membrane-associated with a function in Wnt signaling and in the epithelial mesenchymal transition, while shorter N-terminally-truncated isoforms can be nuclear. [provided by RefSeq, Jan 2013]

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