IQGAP2 (NM_001285460) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC240171 representing NM_001285460 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGTTTGCTTAGTTGAAGAATTGCCACCAACCACTGAATTGGAAGAAGGGCTCCGGAATGGAGTTT ACCTTGCAAAGTTAGCCAAGTTCTTTGCCCCGAAAATGGTATCAGAGAAAAAGATCTATGATGTGGAACA AACACGTTATAAGAAGTCTGGCCTTCATTTTCGACACACAGATAATACCGTCCAGTGGTTAAGAGCGATG GAGTCTATTGGTCTACCCAAGATATTTTATCCAGAAACAACAGATGTCTATGATCGGAAAAACATACCAA GAATGATATATTGCATTCACGCACTGAGTTTGTATCTGTTCAAACTAGGAATAGCACCCCAGATCCAGGA TTTGTTGGGCAAAGTAGACTTCACAGAGGAGGAAATCAGTAATATGAGAAAAGAACTTGAGAAATATGGA ATACAGATGCCATCTTTCAGCAAAATAGGTGGTATTCTGGCCAATGAACTGTCCGTGGATGAAGCTGCAT TACATGCTGCAGTTATAGCCATTAATGAAGCAGTTGAAAAAGGAATAGCAGAGCAAACCGTTGTAACACT AAGAAACCCAAATGCGGTTTTAACTTTAGTGGATGACAACCTTGCACCAGAATATCAGAAAGAACTCTGG GATGCCAAAAAGAAAAAAGAGGAAAATGCAAGACTGAAGAATAGCTGTATTTCAGAAGAAGAAAGAGATG CTTATGAAGAACTGCTGACACAAGCAGAAATCCAAGGCAATATTAATAAAGTCAACAGGCAGGCTGCAGT GGACCATATCAATGCTGTCATTCCGGAAGGTGACCCCGAGAATACGCTGCTTGCACTGAAGAAACCAGAG GCCCAGCTGCCTGCTGTTTATCCCTTTGCTGCTGCCATGTATCAGAACGAACTTTTCAACCTCCAGAAAC AGAACACCATGAACTACTTGGCCCACGAGGAGCTTTTGATTGCTGTGGAAATGTTGTCTGCTGTTGCTTT ACTAAACCAGGCCTTGGAAAGCAACGATCTTGTGTCTGTGCAGAATCAACTCAGAAGCCCCGCAATAGGC TTAAACAATCTGGACAAGGCATATGTGGAACGTTATGCAAACACACTACTCTCTGTTAAACTAGAAGTTT TATCCCAAGGGCAAGATAACTTAAGCTGGAATGAAATTCAGAATTGTATTGATATGGTTAATGCTCAAAT TCAAGAAGAAAATGACCGAGTTGTAGCTGTAGGGTACATCAATGAAGCTATTGATGAAGGGAATCCTTTG AGGACTTTAGAAACTTTGCTCCTACCTACTGCGAATATTAGTGATGTGGACCCAGCCCATGCCCAGCACT ACCAGGATGTTTTATACCATGCTAAATCACAGAAACTCGGAGACTCTGAGAGTGTTTCCAAAGTGCTTTG GCTGGATGAGATACAGCAAGCCGTCGATGATGCCAACGTGGACGAGGACAGAGCAAAACAATGGGTTACT CTGGTGGTTGATGTTAATCAGTGTTTGGAAGGAAAAAAATCAAGTGATATTTTGTCTGTATTGAAGTCTT CCACTTCTAATGCAAATGACATAATCCCGGAGTGTGCTGACAAATACTATGATGCCCTTGTGAAGGCAAA AGAGCTCAAATCTGAAAGAGTGTCTAGTGACGGTTCATGGCTCAAACTCAACCTGCACAAAAAATATGAC TACTATTACAACACTGATTCAAAAGAGAGTTCCTGGGTCACACCTGAATCATGCTTGTATAAAGAATCAT GGCTCACAGGAAAAGAAATCGAGGACATTATTGAGGAAGTCACAGTAGGTTACATTCGTGAGAATATATG GTCTGCTTCAGAAGAGTTGCTTCTTCGCTTTCAAGCCACAAGCTCAGGACCCATCCTTAGGGAAGAGTTT GAAGCTAGAAAATCATTTTTGCATGAACAAGAAGAGAATGTGGTCAAAATACAGGCTTTTTGGAAAGGAT ATAAACAACGGAAGGAGTATATGCACAGGCGGCAAACGTTCATTGATAATACTGATTCTATTGTGAAGAT TCAGTCCTGGTTCCGAATGGCAACTGCAAGAAAGAGCTATCTTTCAAGACTACAGTATTTCAGAGATCAT AATAATGAAATTGTGAAAATACAGTCACTGTTGAGAGCGAACAAAGCTAGAGATGACTACAAAACATTGG TTGGCTCTGAAAACCCACCATTAACAGTAATTCGCAAATTTGTATACCTGCTGGACCAAAGTGATTTGGA TTTCCAGGAGGAACTAGAGGTTGCACGATTAAGGGAAGAAGTAGTGACCAAGATCAGGGCCAATCAACAG CTGGAAAAAGACCTGAACCTGATGGACATCAAGATTGGACTGCTGGTGAAGAACAGGATCACACTAGAGG ATGTAATTTCACACAGTAAAAAGCTGAACAAGAAAAAAGGAGGAGAAATGGAAATACTGAATAACACCGA CAACCAAGGAATAAAAAGTTTGAGTAAGGAGAGGAGAAAAACACTAGAAACATATCAGCAGCTGTTTTAC CTTTTACAGACCAACCCTTTATACTTGGCTAAGCTGATTTTCCAGATGCCACAGAACAAGTCCACTAAAT TTATGGATACTGTTATTTTCACACTATATAATTATGCCTCTAATCAGCGAGAAGAATATCTACTTCTCAA GCTTTTTAAAACTGCTCTGGAGGAAGAAATAAAATCAAAAGTGGACCAGGTACAGGACATAGTTACTGGT AACCCTACAGTCATCAAGATGGTCGTCAGCTTCAATAGAGGTGCCCGGGGACAGAACACCCTGCGCCAAC TCCTGGCTCCAGTGGTAAAAGAGATCATCGACGACAAGTCGCTGATTATCAACACAAACCCTGTAGAGGT GTACAAGGCTTGGGTGAACCAACTAGAAACACAGACTGGAGAGGCCAGCAAGTTGCCTTATGATGTGACC ACAGAACAAGCTCTAACATACCCAGAAGTGAAAAATAAACTGGAGGCTTCCATTGAGAACCTGAGAAGGG TCACCGACAAAGTCCTGAATTCTATCATTTCTTCCCTTGATCTACTGCCTTATGGATTGAGGTATATAGC CAAAGTACTGAAGAATTCGATCCATGAGAAATTCCCCGATGCAACAGAAGATGAGCTATTAAAGATTGTT GGAAACCTCCTGTACTATCGGTACATGAATCCAGCCATTGTAGCTCCAGATGGCTTTGATATCATCGACA TGACAGCTGGAGGTCAGATAAATTCTGACCAAAGGAGAAACTTAGGATCAGTGGCCAAGGTTCTTCAGCA CGCAGCCTCCAACAAGCTGTTTGAAGGAGAAAATGAGCATCTCTCATCTATGAACAATTATTTATCAGAG ACGTATCAGGAATTCAGGAAATATTTCAAAGAAGCATGTAATGTCCCTGAGCCAGAAGAGAAGTTTAATA TGGACAAATACACAGACCTGGTGACAGTCAGCAAACCAGTCATTTATATTTCAATTGAAGAAATCATCAG CACACACTCACTCCTGTTGGAACACCAGGATGCAATTGCCCCTGAGAAAAATGACTTACTGAGTGAATTG CTGGGGTCGCTGGGAGAGGTGCCAACCGTGGAATCTTTTCTTGGGGAAGGAGCAGTTGACCCCAATGACC CTAACAAGGCAAATACACTAAGTCAGCTTTCAAAGACCGAGATTTCTCTTGTCTTGACAAGCAAATATGA CATAGAGGACGGTGAAGCTATAGATAGCCGAAGCCTCATGATAAAGACCAAGAAGCTGATAATTGATGTG ATCCGGAACCAGCCAGGGAACACATTGACAGAAATCTTAGAGACACCAGCAACTGCGCAACAGGAGGTAG ACCATGCCACGGACATGGTGAGCCGTGCAATGATAGATTCCAGGACTCCAGAAGAAATGAAGCATAGCCA ATCTATGATTGAAGATGCACAGCTGCCTCTTGAGCAGAAGAAGAGGAAAATCCAGAGGAATCTTCGGACG TTGGAACAGACTGGACACGTGTCATCCGAAAATAAATACCAAGACATTCTCAATGAGATTGCCAAGGATA TTCGAAATCAAAGAATCTATCGTAAGCTTCGAAAAGCTGAATTGGCAAAACTTCAGCAGACCCTGAATGC ACTTAACAAGAAGGCAGCATTTTATGAAGAGCAAATCAATTATTATGACACCTACATAAAGACTTGTTTA GACAACTTAAAAAGAAAAAATACTCGGAGATCAATTAAACTAGATGGAAAAGGAGAACCCAAAGGGGCGA AGAGAGCGAAGCCAGTGAAGTACACTGCAGCAAAGCTGCATGAGAAAGGTGTCCTGCTAGATATAGATGA TCTTCAAACAAACCAGTTTAAGAATGTTACATTTGATATCATAGCTACTGAAGATGTAGGCATTTTCGAT GTAAGATCAAAATTCCTTGGTGTTGAGATGGAAAAGGTGCAACTCAATATTCAGGATTTACTTCAGATGC AATATGAAGGAGTAGCTGTAATGAAAATGTTTGATAAGGTTAAAGTGAATGTAAACCTTCTCATATACCT GCTGAACAAGAAGTTCTATGGAAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC240171 representing NM_001285460 Red=Cloning site Green=Tags(s) MEVCLVEELPPTTELEEGLRNGVYLAKLAKFFAPKMVSEKKIYDVEQTRYKKSGLHFRHTDNTVQWLRAM ESIGLPKIFYPETTDVYDRKNIPRMIYCIHALSLYLFKLGIAPQIQDLLGKVDFTEEEISNMRKELEKYG IQMPSFSKIGGILANELSVDEAALHAAVIAINEAVEKGIAEQTVVTLRNPNAVLTLVDDNLAPEYQKELW DAKKKKEENARLKNSCISEEERDAYEELLTQAEIQGNINKVNRQAAVDHINAVIPEGDPENTLLALKKPE AQLPAVYPFAAAMYQNELFNLQKQNTMNYLAHEELLIAVEMLSAVALLNQALESNDLVSVQNQLRSPAIG LNNLDKAYVERYANTLLSVKLEVLSQGQDNLSWNEIQNCIDMVNAQIQEENDRVVAVGYINEAIDEGNPL RTLETLLLPTANISDVDPAHAQHYQDVLYHAKSQKLGDSESVSKVLWLDEIQQAVDDANVDEDRAKQWVT LVVDVNQCLEGKKSSDILSVLKSSTSNANDIIPECADKYYDALVKAKELKSERVSSDGSWLKLNLHKKYD YYYNTDSKESSWVTPESCLYKESWLTGKEIEDIIEEVTVGYIRENIWSASEELLLRFQATSSGPILREEF EARKSFLHEQEENVVKIQAFWKGYKQRKEYMHRRQTFIDNTDSIVKIQSWFRMATARKSYLSRLQYFRDH NNEIVKIQSLLRANKARDDYKTLVGSENPPLTVIRKFVYLLDQSDLDFQEELEVARLREEVVTKIRANQQ LEKDLNLMDIKIGLLVKNRITLEDVISHSKKLNKKKGGEMEILNNTDNQGIKSLSKERRKTLETYQQLFY LLQTNPLYLAKLIFQMPQNKSTKFMDTVIFTLYNYASNQREEYLLLKLFKTALEEEIKSKVDQVQDIVTG NPTVIKMVVSFNRGARGQNTLRQLLAPVVKEIIDDKSLIINTNPVEVYKAWVNQLETQTGEASKLPYDVT TEQALTYPEVKNKLEASIENLRRVTDKVLNSIISSLDLLPYGLRYIAKVLKNSIHEKFPDATEDELLKIV GNLLYYRYMNPAIVAPDGFDIIDMTAGGQINSDQRRNLGSVAKVLQHAASNKLFEGENEHLSSMNNYLSE TYQEFRKYFKEACNVPEPEEKFNMDKYTDLVTVSKPVIYISIEEIISTHSLLLEHQDAIAPEKNDLLSEL LGSLGEVPTVESFLGEGAVDPNDPNKANTLSQLSKTEISLVLTSKYDIEDGEAIDSRSLMIKTKKLIIDV IRNQPGNTLTEILETPATAQQEVDHATDMVSRAMIDSRTPEEMKHSQSMIEDAQLPLEQKKRKIQRNLRT LEQTGHVSSENKYQDILNEIAKDIRNQRIYRKLRKAELAKLQQTLNALNKKAAFYEEQINYYDTYIKTCL DNLKRKNTRRSIKLDGKGEPKGAKRAKPVKYTAAKLHEKGVLLDIDDLQTNQFKNVTFDIIATEDVGIFD VRSKFLGVEMEKVQLNIQDLLQMQYEGVAVMKMFDKVKVNVNLLIYLLNKKFYGK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001285460
ORF Size	4575 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001285460.1, NP_001272389.1
RefSeq Size	5460 bp
RefSeq ORF	4578 bp
Locus ID	10788
UniProt ID	Q13576
Protein Families	Druggable Genome
Protein Pathways	Regulation of actin cytoskeleton
MW	174.9 kDa
Gene Summary	This gene encodes a member of the IQGAP family. The encoded protein contains three IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. This protein interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. It also acts as a tumor suppressor and has been found to play a role in regulating innate antiviral responses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2017]

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