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AP4S1 (NM_007077) Human Tagged ORF Clone
CAT＃: RG201527
- TrueORF®
AP4S1 (tGFP-tagged) - Human adaptor-related protein complex 4, sigma 1 subunit (AP4S1), transcript variant 1
ORF Plasmid: DDK
"NM_007077" in other vectors (5)
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CNY 2,800.00
CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AP47B; CLA20; CLAPS4; CPSQ6; SPG52 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG201527 representing NM_007077
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATAAAATTTTTCCTCATGGTGAATAAACAAGGGCAGACTCGACTTTCTAAGTACTATGAACATGTGG ATATTAATAAGCGTACACTTCTGGAAACAGAAGTCATAAAGAGCTGTCTCTCTCGATCCAATGAACAATG CTCTTTCATTGAATATAAGGATTTTAAGCTGATATATCGGCAGTATGCAGCTCTCTTCATTGTGGTTGGA GTTAATGACACTGAGAACGAGATGGCTATTTATGAATTCATTCATAACTTTGTGGAAGTTTTAGATGAGT ATTTCAGCCGAGTGAGTGAATTAGATGTATCCTTTTTCAATACTGTTTTCCACAGTACTTGGCAAATGCA CTCTGGTCCTTATCAGGAACCAATTGATGAACTTCCCAAAATATGCTCAGCCCTGGAGCCCCAGCAGACT TGCTTTTCTCCAGACAGTTCATCATTCAAAGGAGCTGCCTCCACCACCCCCATCTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG201527 representing NM_007077
Red=Cloning site Green=Tags(s) MIKFFLMVNKQGQTRLSKYYEHVDINKRTLLETEVIKSCLSRSNEQCSFIEYKDFKLIYRQYAALFIVVG VNDTENEMAIYEFIHNFVEVLDEYFSRVSELDVSFFNTVFHSTWQMHSGPYQEPIDELPKICSALEPQQT CFSPDSSSFKGAASTTPIY TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007077 |
| ORF Size | 477 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007077.4 |
| RefSeq Size | 1587 bp |
| RefSeq ORF | 480 bp |
| Locus ID | 11154 |
| UniProt ID | Q9Y587 |
| Domains | Clat_adaptor_s |
| Protein Pathways | Lysosome |
| Gene Summary | This gene encodes a member of the adaptor complexes small subunit protein family. These proteins are components of the heterotetrameric adaptor protein complexes, which play important roles in the secretory and endocytic pathways by mediating vesicle formation and sorting of integral membrane proteins. The encoded protein is the small subunit of adaptor protein complex-4, which is associated with both clathrin- and nonclathrin-coated vesicles. Mutations in this gene are associated with spastic quadriplegic cerebral palsy-6. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 6. [provided by RefSeq, Dec 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201527 | AP4S1 (Myc-DDK-tagged)-Human adaptor-related protein complex 4, sigma 1 subunit (AP4S1), transcript variant 1 |
CNY 1,200.00 |
|
| RC201527L3 | Lenti ORF clone of Human adaptor-related protein complex 4, sigma 1 subunit (AP4S1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201527L4 | Lenti ORF clone of Human adaptor-related protein complex 4, sigma 1 subunit (AP4S1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| SC115711 | AP4S1 (untagged)-Human adaptor-related protein complex 4, sigma 1 subunit (AP4S1), transcript variant 1 |
CNY 2,950.00 |
|
| SC322208 | AP4S1 (untagged)-Human adaptor-related protein complex 4, sigma 1 subunit (AP4S1), transcript variant 1 |
CNY 1,200.00 |
