SF3B14 (SF3B6) (NM_016047) Human Tagged ORF Clone

CAT＃: RG201866

TrueORF^®

SF3B14 (tGFP-tagged) - Human splicing factor 3B, 14 kDa subunit (SF3B14)

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"NM_016047" in other vectors (6)

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CNY 2,800.00

CNY 4,370.00

货期*

现货

规格

Product images

经常一起买 (5)

PS100010

pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug

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TA150041

Mouse monoclonal turboGFP antibody, clone OTI2H8

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CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

TA811148

SF3B14 mouse monoclonal antibody,clone OTI8A1

CNY 1,999.00
CNY 2,700.00

【特别福利】购一抗正装产品，免费领10ul抗体试用装

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	TurboGFP
Synonyms	CGI-110; HSPC175; Ht006; P14; SAP14; SAP14a; SF3B14; SF3B14a
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RG201866 representing NM_016047 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGATGCAAGCGGCCAAGAGGGCGAACATTCGACTTCCACCTGAAGTAAATCGGATATTGTATATAA GAAATTTGCCATACAAAATCACAGCTGAAGAAATGTATGATATATTTGGGAAATATGGACCTATTCGTCA AATCAGAGTGGGGAACACACCTGAAACTAGAGGAACAGCTTATGTGGTCTATGAGGACATCTTTGATGCC AAGAATGCATGTGATCACCTATCGGGATTCAATGTTTGTAACAGATACCTTGTGGTTTTGTACTATAATG CCAACAGGGCATTTCAGAAGATGGACACAAAGAAGAAGGAGGAACAGTTGAAGCTTCTCAAGGAGAAATA TGGCATCAACACAGATCCACCAAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >RG201866 representing NM_016047 Red=Cloning site Green=Tags(s) MAMQAAKRANIRLPPEVNRILYIRNLPYKITAEEMYDIFGKYGPIRQIRVGNTPETRGTAYVVYEDIFDA KNACDHLSGFNVCNRYLVVLYYNANRAFQKMDTKKKEEQLKLLKEKYGINTDPPK TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_016047
ORF Size	375 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_016047.4
RefSeq Size	783 bp
RefSeq ORF	378 bp
Locus ID	51639
UniProt ID	Q9Y3B4
Domains	RRM
Protein Pathways	Spliceosome
Gene Summary	This gene encodes a 14 kDa protein subunit of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. This 14 kDa protein interacts directly with subunit 1 of the splicing factor 3b complex. This 14 kDa protein also interacts directly with the adenosine that carries out the first transesterification step of splicing at the pre-mRNA branch site. [provided by RefSeq, Jul 2008]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC201866	SF3B14 (Myc-DDK-tagged)-Human splicing factor 3B, 14 kDa subunit (SF3B14)	10 ug 产品选项验证失败所有选项都是必需的	CNY 1,200.00 CNY 3,705.00
RC201866L1	Lenti ORF clone of Human splicing factor 3B, 14 kDa subunit (SF3B14), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,600.00 CNY 5,890.00
RC201866L2	Lenti ORF clone of Human splicing factor 3B, 14 kDa subunit (SF3B14), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC201866L3	Lenti ORF clone of Human splicing factor 3B, 14 kDa subunit (SF3B14), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC201866L4	Lenti ORF clone of Human splicing factor 3B, 14 kDa subunit (SF3B14), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
SC107008	SF3B14 (untagged)-Human splicing factor 3B, 14 kDa subunit (SF3B14)	10 ug 产品选项验证失败所有选项都是必需的	CNY 1,200.00 CNY 2,950.00

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