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ALOX12B (NM_001139) Human Tagged ORF Clone
CAT＃: RG208343
- TrueORF®
ALOX12B (tGFP-tagged) - Human arachidonate 12-lipoxygenase, 12R type (ALOX12B)
ORF Plasmid: DDK
"NM_001139" in other vectors (4)
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CNY 9,304.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 12R-LOX; ARCI2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG208343 representing NM_001139
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACCTACAAAGTCAGGGTGGCCACAGGCACCGACCTCTTGTCGGGAACACGGGACTCCATCTCAC TGACCATTGTGGGGACACAAGGAGAGAGCCATAAGCAGCTGCTGAACCACTTTGGGAGAGACTTTGCAAC TGGGGCGGTGGGCCAGTACACCGTGCAGTGCCCTCAGGACCTGGGTGAGCTCATCATCATCCGCCTGCAC AAAGAGCGGTACGCCTTCTTCCCCAAGGACCCTTGGTACTGCAACTATGTGCAGATCTGTGCCCCCAACG GCCGTATCTACCACTTCCCCGCCTACCAGTGGATGGATGGCTACGAGACCCTGGCACTCCGGGAGGCCAC AGGAAAGACAACAGCAGATGACTCGCTCCCCGTCCTCCTGGAGCACAGAAAAGAGGAGATCAGAGCCAAG CAGGACTTCTACCACTGGCGAGTCTTTCTTCCTGGCCTGCCCAGCTATGTGCACATTCCCAGTTACCGCC CTCCGGTGCGGAGGCATCGCAACCCCAACCGGCCTGAGTGGAATGGCTATATTCCGGGATTCCCAATTCT CATCAACTTTAAGGCCACCAAGTTCCTGAACTTAAATCTCCGCTACTCCTTCCTCAAGACGGCCTCCTTC TTCGTCCGCCTGGGGCCCATGGCACTGGCTTTCAAAGTCCGCGGCCTGTTGGACTGCAAACATTCGTGGA AGAGGCTGAAGGACATTAGGAAAATTTTCCCTGGCAAGAAATCTGTCGTCTCCGAGTACGTGGCCGAGCA CTGGGCAGAGGACACCTTCTTTGGGTACCAGTACCTCAACGGCGTCAACCCCGGCCTGATCCGCCGCTGC ACGCGGATCCCAGACAAGTTCCCCGTCACAGACGACATGGTGGCTCCGTTCCTGGGCGAGGGAACGTGCT TGCAAGCGGAGCTGGAGAAGGGGAACATTTACCTGGCCGACTACCGCATCATGGAGGGCATCCCCACCGT GGAGCTCAGCGGCCGGAAGCAGCACCACTGCGCCCCCCTCTGCCTGCTGCACTTTGGACCCGAGGGCAAG ATGATGCCCATCGCCATCCAGCTCAGCCAGACCCCTGGGCCAGATTGCCCCATCTTCCTGCCCAGTGATT CTGAGTGGGACTGGCTGCTAGCCAAGACGTGGGTACGCTATGCGGAGTTCTACAGCCACGAGGCCATCGC CCACCTGCTGGAGACACACCTCATTGCTGAGGCCTTCTGCCTGGCCTTGCTGAGGAACCTGCCCATGTGC CACCCCCTCTACAAGCTCCTCATCCCCCATACCCGATACACCGTCCAGATCAACAGCATTGGCCGGGCCG TTCTCCTCAATGAGGGGGGGCTCTCTGCCAAGGGCATGTCCCTGGGCGTGGAAGGCTTTGCTGGGGTGAT GGTACGGGCTCTGTCGGAGCTCACCTATGACAGCCTCTACCTCCCCAATGACTTTGTGGAGCGTGGGGTC CAGGACCTGCCTGGATATTACTACCGCGATGACAGCTTGGCGGTGTGGAATGCACTGGAGAAGTATGTGA CGGAGATCATCACCTATTATTACCCGAGTGACGCAGCCGTGGAGGGTGATCCGGAATTGCAGTCTTGGGT GCAGGAAATATTTAAAGAGTGCCTCCTGGGGCGGGAGAGCTCAGGCTTCCCTAGGTGCTTGCGAACCGTG CCTGAGCTGATCCGATATGTCACTATAGTCATCTACACCTGCTCTGCCAAGCACGCTGCTGTCAACACAG GCCAGATGGAGTTCACCGCCTGGATGCCCAACTTCCCAGCGTCCATGCGGAATCCACCGATTCAGACTAA GGGGCTGACCACTCTGGAGACCTTCATGGACACGTTGCCGGATGTGAAGACCACGTGCATCACGCTGCTG GTGCTCTGGACCCTCAGCCGAGAGCCTGACGACAGGCGGCCCCTGGGACACTTCCCGGACATTCACTTCG TGGAGGAGGCCCCGCGGAGGAGCATAGAGGCGTTCCGCCAGCGCCTGAACCAGATCTCACACGACATCCG CCAGCGCAACAAGTGCCTTCCCATCCCCTACTACTACCTGGACCCGGTGCTGATTGAGAACAGCATTTCT ATT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG208343 representing NM_001139
Red=Cloning site Green=Tags(s) MATYKVRVATGTDLLSGTRDSISLTIVGTQGESHKQLLNHFGRDFATGAVGQYTVQCPQDLGELIIIRLH KERYAFFPKDPWYCNYVQICAPNGRIYHFPAYQWMDGYETLALREATGKTTADDSLPVLLEHRKEEIRAK QDFYHWRVFLPGLPSYVHIPSYRPPVRRHRNPNRPEWNGYIPGFPILINFKATKFLNLNLRYSFLKTASF FVRLGPMALAFKVRGLLDCKHSWKRLKDIRKIFPGKKSVVSEYVAEHWAEDTFFGYQYLNGVNPGLIRRC TRIPDKFPVTDDMVAPFLGEGTCLQAELEKGNIYLADYRIMEGIPTVELSGRKQHHCAPLCLLHFGPEGK MMPIAIQLSQTPGPDCPIFLPSDSEWDWLLAKTWVRYAEFYSHEAIAHLLETHLIAEAFCLALLRNLPMC HPLYKLLIPHTRYTVQINSIGRAVLLNEGGLSAKGMSLGVEGFAGVMVRALSELTYDSLYLPNDFVERGV QDLPGYYYRDDSLAVWNALEKYVTEIITYYYPSDAAVEGDPELQSWVQEIFKECLLGRESSGFPRCLRTV PELIRYVTIVIYTCSAKHAAVNTGQMEFTAWMPNFPASMRNPPIQTKGLTTLETFMDTLPDVKTTCITLL VLWTLSREPDDRRPLGHFPDIHFVEEAPRRSIEAFRQRLNQISHDIRQRNKCLPIPYYYLDPVLIENSIS I TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001139 |
| ORF Size | 2103 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001139.3 |
| RefSeq Size | 2469 bp |
| RefSeq ORF | 2106 bp |
| Locus ID | 242 |
| UniProt ID | O75342 |
| Protein Families | Druggable Genome |
| Protein Pathways | Arachidonic acid metabolism, Metabolic pathways |
| Gene Summary | This gene encodes an enzyme involved in the conversion of arachidonic acid to 12R-hydroxyeicosatetraenoic acid. Mutations in this gene are associated with nonbullous congenital ichthyosiform erythroderma. [provided by RefSeq, Sep 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208343 | ALOX12B (Myc-DDK-tagged)-Human arachidonate 12-lipoxygenase, 12R type (ALOX12B) |
CNY 7,704.00 |
|
| RC208343L3 | Lenti ORF clone of Human arachidonate 12-lipoxygenase, 12R type (ALOX12B), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC208343L4 | Lenti ORF clone of Human arachidonate 12-lipoxygenase, 12R type (ALOX12B), mGFP tagged |
CNY 7,700.00 |
|
| SC126150 | ALOX12B (untagged)-Human arachidonate 12-lipoxygenase, 12R type (ALOX12B) |
CNY 7,712.00 |
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