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ADCY10 (NM_018417) Human Tagged ORF Clone
CAT＃: RG214876
- TrueORF®
ADCY10 (tGFP-tagged) - Human adenylate cyclase 10 (soluble) (ADCY10), transcript variant 1
ORF Plasmid: DDK
"NM_018417" in other vectors (4)
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CNY 17,670.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | HCA2; HEL-S-7a; hsAC; SAC; SACI; Sacy |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG214876 representing NM_018417
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACACTCCAAAAGAAGAATTCCAGGACTGGCCCATAGTCAGAATAGCAGCTCATTTACCAGACCTCA TTGTCTATGGACATTTCTCCCCAGAGCGACCCTTTATGGATTATTTTGACGGAGTCCTGATGTTTGTTGA TATTTCAGGTTTTACTGCAATGACTGAGAAGTTCAGCAGTGCCATGTACATGGACAGAGGGGCTGAGCAG TTGGTGGAGATCCTCAACTACCACATAAGTGCAATAGTGGAGAAAGTGTTGATTTTTGGAGGAGACATCC TGAAATTTGCAGGTGATGCACTGCTAGCCCTGTGGAGGGTGGAGCGAAAGCAGCTGAAAAACATTATCAC AGTGGTAATTAAATGTAGCCTGGAGATCCATGGATTGTTTGAGACCCAGGAGTGGGAAGAAGGCCTAGAC ATCCGAGTCAAGATAGGACTGGCTGCTGGCCACATCAGCATGTTGGTCTTTGGAGATGAAACACACAGCC ACTTTCTGGTGATTGGTCAGGCAGTGGACGATGTGCGCCTTGCCCAGAACATGGCTCAGATGAATGATGT TATTCTGTCACCAAACTGCTGGCAGCTCTGTGACCGGAGCATGATTGAAATTGAGAGTGTTCCAGATCAG AGAGCAGTTAAGGTTAACTTCTTAAAACCACCCCCCAATTTTAATTTTGATGAATTTTTCACAAAGTGTA CGACCTTCATGCATTATTATCCTTCTGGTGAGCACAAAAACCTCCTGAGGCTTGCATGCACGCTGAAGCC TGATCCTGAACTGGAGATGTCCCTACAAAAGTATGTGATGGAAAGCATTTTGAAGCAGATTGATAACAAA CAGCTTCAGGGCTATTTATCTGAGCTTCGCCCAGTGACGATTGTGTTTGTGAACCTGATGTTTGAAGACC AAGACAAAGCAGAAGAGATAGGCCCAGCCATCCAGGATGCCTATATGCACATCACTTCTGTCCTGAAGAT CTTCCAAGGCCAAATCAATAAAGTCTTCATGTTTGACAAGGGCTGCTCTTTCCTCTGTGTCTTTGGCTTC CCTGGGGAAAAGGTACCTGACGAGCTCACTCATGCTCTGGAATGTGCTATGGATATATTTGACTTCTGCT CTCAAGTCCACAAAATCCAAACTGTATCCATCGGTGTTGCCAGTGGGATTGTCTTCTGTGGGATCGTTGG ACACACTGTGAGACACGAGTACACAGTCATTGGTCAAAAAGTCAACTTAGCTGCCAGGATGATGATGTAC TACCCAGGAATTGTGACCTGCGACTCTGTCACCTACAATGGGAGCAACCTACCAGCGTACTTTTTTAAAG AGCTTCCAAAGAAAGTTATGAAAGGTGTTGCAGATTCTGGACCATTGTATCAGTATTGGGGCCGTACTGA GAAAGTCATGTTTGGTATGGCGTGCCTCATCTGCAACAGAAAGGAGGATTACCCTTTGCTGGGACGTAAT AAAGAGATCAACTACTTCATGTATACTATGAAGAAATTTTTGATATCTAACAGCAGCCAAGTCTTAATGT ATGAGGGATTACCAGGATATGGAAAAAGCCAGATACTTATGAAAATTGAGTACCTGGCCCAAGGTAAGAA TCACAGGATTATTGCCATTTCATTGAATAAGATCAGCTTCCATCAAACTTTCTATACCATCCAGATGTTC ATGGCCAATGTCCTAGGCCTAGACACTTGTAAACATTATAAAGAACGACAGACCAACCTTCGAAATAAAG TCATGACACTGTTGGATGAAAAGTTCTACTGTCTTCTTAATGACATTTTCCATGTTCAGTTCCCTATTTC TCGGGAGATTTCCAGGATGAGCACCTTGAAAAAGCAAAAACAATTGGAAATATTGTTTATGAAGATCTTG AAGCTGATAGTGAAAGAGGAAAGGATTATTTTTATCATTGATGAGGCCCAGTTTGTGGATTCGACCTCCT GGAGATTTATGGAGAAGCTTATCCGGACTCTTCCTATCTTCATCATTATGTCCCTGTGTCCCTTCGTTAA CATTCCCTGTGCAGCTGCCAGGGCCGTAATAAAGAACAGGAACACCACCTACATTGTCGTTGGTGCAGTA CAGCCTAACGACATCTCCAACAAGATCTGTCTTGACCTCAATGTGAGCTGCATCTCCAAAGAACTGGACT CGTACCTGGGGGAGGGAAGCTGTGGGATTCCATTTTACTGTGAAGAATTGCTTAAAAACCTGGAACATCA TGAGGTACTCGTTTTCCAACAAACGGAGTCTGAGGAAAAGACAAATAGGACCTGGAATAACCTGTTCAAG TATTCCATTAAGCTAACAGAGAAGTTAAACATGGTTACTCTCCATAGTGATAAGGAAAGTGAAGAAGTCT GTCACCTCACAAGTGGTGTCAGACTGAAAAACCTGTCACCTCCAACGTCATTAAAAGAAATCTCTCTGAT CCAGCTGGATAGCATGAGACTTTCCCACCAAATGCTGGTGAGATGTGCTGCCATCATTGGCCTGACCTTC ACCACTGAGTTGTTGTTTGAGATTCTCCCCTGTTGGAATATGAAGATGATGATCAAGACCCTGGCAACCC TAGTGGAATCTAACATTTTTTATTGTTTCCGGAATGGCAAGGAGCTTCAAAAGGCCCTGAAACAGAATGA TCCCTCATTTGAGGTGCACTATCGTTCCTTGTCTCTGAAGCCCAGTGAAGGGATGGATCACGGTGAAGAG GAACAGCTTCGTGAACTGGAGAATGAGGTGATCGAGTGCCACAGGATTCGATTCTGTAACCCTATGATGC AGAAAACAGCCTACGAGCTGTGGCTCAAGGACCAGAGAAAAGCCATGCACTTGAAATGTGCCCGCTTTTT AGAAGAAGATGCCCACAGATGTGACCACTGCCGAGGCAGGGACTTCATTCCCTATCATCACTTCACAGTG AATATTCGGCTCAACGCTTTAGACATGGATGCCATTAAAAAGATGGCTATGTCTCATGGATTTAAAACTG AAGAAAAGCTTATCTTGTCCAACTCAGAGATTCCTGAGACATCTGCATTTTTTCCTGAAAATCGCAGTCC TGAAGAAATAAGAGAAAAGATCTTGAATTTCTTTGACCACGTTTTAACAAAAATGAAGACATCTGACGAA GACATTATCCCTCTGGAATCTTGCCAGTGTGAAGAAATCCTAGAGATTGTCATCTTGCCTCTGGCCCACC ATTTTCTGGCTTTGGGAGAAAATGACAAAGCCTTATATTACTTCTTAGAAATTGCATCTGCTTATCTCAT CTTTTGTGATAACTACATGGCATACATGTATTTGAATGAAGGACAGAAGTTGCTAAAAACTCTCAAGAAG GACAAATCTTGGAGCCAGACATTTGAGTCTGCCACCTTTTACAGCCTCAAAGGTGAGGTCTGTTTCAATA TGGGCCAGATAGTGCTTGCCAAGAAAATGCTGAGGAAGGCACTGAAGCTCCTCAACCGAATCTTTCCTTA CAACTTAATCTCCTTGTTTCTCCATATCCATGTCGAGAAAAACAGACACTTTCATTATGTGAATCGGCAG GCCCAAGAGAGCCCACCTCCAGGGAAGAAGAGGCTGGCACAACTTTACCGGCAAACTGTCTGCCTTTCCT TGCTGTGGCGCATCTATAGCTACAGTTATCTTTTTCACTGCAAGTATTATGCCCACCTGGCAGTTATGAT GCAAATGAATACTGCACTGGAAACTCAAAATTGTTTCCAGATCATTAAGGCTTACCTAGACTATTCGCTA TACCACCACCTGGCTGGCTACAAAGGTGTGTGGTTCAAATATGAAGTCATGGCCATGGAGCACATCTTCA ACCTCCCCCTGAAAGGCGAGGGCATTGAAATCGTGGCATACGTGGCTGAGACACTGGTCTTCAACAAGCT CATAATGGGACACCTGGATTTGGCCATTGAGTTAGGCTCCCGAGCCCTTCAGATGTGGGCACTGCTCCAG AATCCCAACCGACATTATCAGTCCCTCTGCAGACTTAGCAGATGTCTCCTTCTGAACAGCAGATACCCGC AATTGATCCAGGTGCTGGGGCGGCTGTGGGAGCTTTCTGTAACACAGGAACACATCTTCAGCAAGGCATT TTTCTATTTTGTCTGCTTGGACATCCTGCTTTATTCTGGTTTTGTTTATAGAACATTTGAAGAATGTTTG GAATTCATACACCAATACGAAAACAACAGAATCCTCAAGTTCCACAGTGGACTCCTCCTGGGACTTTATT CCTCTGTAGCTATCTGGTATGCCAGACTTCAGGAATGGGACAACTTTTACAAATTTTCCAATAGAGCTAA AAATCTTTTGCCAAGAAGAACCATGACACTTACTTACTATGACGGAATATCTAGGTACATGGAGGGGCAA GTTCTTCACCTTCAAAAACAAATCAAAGAACAGTCAGAGAATGCCCAAGCCAGTGGGGAGGAGCTACTCA AGAACTTGGAGAATCTGGTGGCTCAAAATACCACTGGCCCTGTCTTTTGCCCAAGGCTCTACCACCTGAT GGCTTACGTCTGTATATTAATGGGAGATGGGCAGAAATGTGGCCTCTTCCTGAACACAGCCTTGCGGCTC TCTGAAACACAGGGGAATATACTGGAGAAATGCTGGCTGAACATGAACAAAGAATCATGGTACTCAACCT CTGAGTTAAAAGAAGACCAATGGCTTCAGACGATCTTGAGTCTCCCATCATGGGAAAAAATTGTAGCAGG CAGGGTAAACATTCAGGATCTTCAAAAAAACAAATTCCTGATGAGAGCTAATACCGTGGACAATCATTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG214876 representing NM_018417
Red=Cloning site Green=Tags(s) MNTPKEEFQDWPIVRIAAHLPDLIVYGHFSPERPFMDYFDGVLMFVDISGFTAMTEKFSSAMYMDRGAEQ LVEILNYHISAIVEKVLIFGGDILKFAGDALLALWRVERKQLKNIITVVIKCSLEIHGLFETQEWEEGLD IRVKIGLAAGHISMLVFGDETHSHFLVIGQAVDDVRLAQNMAQMNDVILSPNCWQLCDRSMIEIESVPDQ RAVKVNFLKPPPNFNFDEFFTKCTTFMHYYPSGEHKNLLRLACTLKPDPELEMSLQKYVMESILKQIDNK QLQGYLSELRPVTIVFVNLMFEDQDKAEEIGPAIQDAYMHITSVLKIFQGQINKVFMFDKGCSFLCVFGF PGEKVPDELTHALECAMDIFDFCSQVHKIQTVSIGVASGIVFCGIVGHTVRHEYTVIGQKVNLAARMMMY YPGIVTCDSVTYNGSNLPAYFFKELPKKVMKGVADSGPLYQYWGRTEKVMFGMACLICNRKEDYPLLGRN KEINYFMYTMKKFLISNSSQVLMYEGLPGYGKSQILMKIEYLAQGKNHRIIAISLNKISFHQTFYTIQMF MANVLGLDTCKHYKERQTNLRNKVMTLLDEKFYCLLNDIFHVQFPISREISRMSTLKKQKQLEILFMKIL KLIVKEERIIFIIDEAQFVDSTSWRFMEKLIRTLPIFIIMSLCPFVNIPCAAARAVIKNRNTTYIVVGAV QPNDISNKICLDLNVSCISKELDSYLGEGSCGIPFYCEELLKNLEHHEVLVFQQTESEEKTNRTWNNLFK YSIKLTEKLNMVTLHSDKESEEVCHLTSGVRLKNLSPPTSLKEISLIQLDSMRLSHQMLVRCAAIIGLTF TTELLFEILPCWNMKMMIKTLATLVESNIFYCFRNGKELQKALKQNDPSFEVHYRSLSLKPSEGMDHGEE EQLRELENEVIECHRIRFCNPMMQKTAYELWLKDQRKAMHLKCARFLEEDAHRCDHCRGRDFIPYHHFTV NIRLNALDMDAIKKMAMSHGFKTEEKLILSNSEIPETSAFFPENRSPEEIREKILNFFDHVLTKMKTSDE DIIPLESCQCEEILEIVILPLAHHFLALGENDKALYYFLEIASAYLIFCDNYMAYMYLNEGQKLLKTLKK DKSWSQTFESATFYSLKGEVCFNMGQIVLAKKMLRKALKLLNRIFPYNLISLFLHIHVEKNRHFHYVNRQ AQESPPPGKKRLAQLYRQTVCLSLLWRIYSYSYLFHCKYYAHLAVMMQMNTALETQNCFQIIKAYLDYSL YHHLAGYKGVWFKYEVMAMEHIFNLPLKGEGIEIVAYVAETLVFNKLIMGHLDLAIELGSRALQMWALLQ NPNRHYQSLCRLSRCLLLNSRYPQLIQVLGRLWELSVTQEHIFSKAFFYFVCLDILLYSGFVYRTFEECL EFIHQYENNRILKFHSGLLLGLYSSVAIWYARLQEWDNFYKFSNRAKNLLPRRTMTLTYYDGISRYMEGQ VLHLQKQIKEQSENAQASGEELLKNLENLVAQNTTGPVFCPRLYHLMAYVCILMGDGQKCGLFLNTALRL SETQGNILEKCWLNMNKESWYSTSELKEDQWLQTILSLPSWEKIVAGRVNIQDLQKNKFLMRANTVDNHF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018417 |
| ORF Size | 4830 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018417.2, NP_060887.1 |
| RefSeq Size | 5061 bp |
| RefSeq ORF | 4833 bp |
| Locus ID | 55811 |
| UniProt ID | Q96PN6 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Purine metabolism |
| Gene Summary | The protein encoded by this gene belongs to a distinct class of adenylyl cyclases that is soluble and insensitive to G protein or forskolin regulation. Activity of this protein is regulated by bicarbonate. Variation at this gene has been observed in patients with absorptive hypercalciuria. Alternatively spliced transcript variants encoding different isoforms have been observed. There is a pseudogene of this gene on chromosome 6. [provided by RefSeq, Jul 2014] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214876 | ADCY10 (Myc-DDK-tagged)-Human adenylate cyclase 10 (soluble) (ADCY10), transcript variant 1 |
CNY 16,520.00 |
|
| RC214876L3 | Lenti-ORF clone of ADCY10 (Myc-DDK-tagged)-Human adenylate cyclase 10 (soluble) (ADCY10), transcript variant 1 |
CNY 17,960.00 |
|
| RC214876L4 | Lenti-ORF clone of ADCY10 (mGFP-tagged)-Human adenylate cyclase 10 (soluble) (ADCY10), transcript variant 1 |
CNY 17,960.00 |
|
| SC304592 | ADCY10 (untagged)-Human adenylate cyclase 10 (soluble) (ADCY10), transcript variant 1 |
CNY 17,632.00 |
