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LAMB4 (NM_007356) Human Tagged ORF Clone
CAT＃: RG215486
- TrueORF®
LAMB4 (tGFP-tagged) - Human laminin, beta 4 (LAMB4)
ORF Plasmid: DDK
"NM_007356" in other vectors (2)
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CNY 15,200.00
货期*
8周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG215486 representing NM_007356
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAATTTCAACTGACCCTTTTTTTGCACCTTGGGTGGCTCAGTTACTCAAAAGCTCAAGATGACTGCA ACAGGGGTGCCTGTCATCCCACCACTGGTGATCTCCTGGTGGGCAGGAACACGCAGCTTATGGCTTCTTC TACCTGTGGGCTGAGCAGAGCCCAGAAATACTGCATCCTCAGTTACCTGGAGGGGGAACAAAAATGCTCC ATCTGTGACTCTAGATTTCCATATGATCCGTATGACCAACCCAACAGCCACACCATTGAGAATGTCACTG TAAGTTTTGAACCAGACAGAGAAAAGAAATGGTGGCAATCTGAAAATGGTCTTGATCATGTCAGCATCAG ACTGGACTTAGAGGCATTATTTCGGTTCAGCCACCTTATCCTGACCTTTAAGACTTTTCGGCCTGCTGCA ATGTTAGTTGAACGTTCCACAGACTATGGACACAACTGGAAAGTGTTCAAATATTTTGCAAAAGACTGTG CCACTTCCTTTCCTAACATCACATCTGGCCAGGCCCAGGGAGTGGGAGACATTGTTTGTGACTCCAAATA CTCGGATATTGAACCCTCAACAGGTGGAGAGGTTGTTTTAAAAGTTTTGGATCCCAGTTTTGAAATTGAA AACCCTTATAGCCCCTACATCCAAGACCTTGTGACATTGACAAACCTGAGGATAAACTTTACCAAGCTCC ACACCCTTGGGGATGCTTTGCTTGGAAGGAGGCAAAATGATTCCCTTGATAAATACTACTATGCTCTGTA CGAGATGATTGTTCGGGGAAGCTGCTTTTGCAATGGCCATGCTAGCGAATGTCGCCCTATGCAGAAGATG CGGGGAGATGTTTTCAGCCCTCCTGGAATGGTTCACGGTCAGTGTGTGTGTCAGCACAATACAGATGGTC CGAACTGTGAGAGATGCAAGGACTTCTTCCAGGATGCTCCTTGGAGGCCAGCTGCAGACCTCCAGGACAA CGCTTGCAGATCGTGCAGCTGTAATAGCCACTCCAGCCGCTGTCACTTTGACATGACTACGTACCTGGCA AGCGGTGGCCTCAGCGGGGGCGTGTGTGAAGACTGCCAGCACAACACTGAGGGGCAGCACTGCGACCGCT GCAGACCCCTCTTCTACAGGGACCCGCTCAAGACCATCTCAGATCCCTACGCGTGCATTCCTTGTGAATG TGACCCCGATGGGACCATATCTGGTGGCATTTGTGTGAGCCACTCTGATCCTGCCTTAGGGTCTGTGGCC GGCCAGTGCCTTTGTAAAGAGAACGTGGAAGGAGCCAAATGCGACCAGTGCAAACCCAACCACTACGGAC TAAGCGCCACCGACCCCCTGGGCTGCCAGCCCTGCGACTGTAACCCCCTTGGGAGTCTGCCATTCTTGAC CTGTGATGTGGATACAGGCCAATGCTTGTGCCTGTCATATGTCACCGGAGCACACTGCGAAGAATGCACT GTTGGATACTGGGGCCTGGGAAATCATCTCCATGGGTGTTCTCCCTGTGACTGTGATATTGGAGGTGCTT ATTCTAACGTGTGCTCACCCAAGAATGGGCAGTGTGAATGCCGCCCACATGTCACTGGCCGTAGCTGCTC TGAACCAGCCCCTGGCTACTTCTTTGCTCCTTTGAATTTCTATCTCTACGAGGCAGAGGAAGCCACAACA CTCCAAGGACTGGCGCCTTTGGGCTCGGAGACGTTTGGCCAGAGTCCTGCTGTTCACGTTGTTTTAGGAG AGCCAGTTCCTGGGAACCCTGTTACATGGACTGGACCTGGATTTGCCAGGGTTCTCCCTGGGGCTGGCTT GAGATTTGCTGTCAACAACATTCCCTTTCCTGTGGACTTCACCATTGCCATTCACTATGAAACCCAGTCT GCAGCTGACTGGACTGTCCAGATTGTGGTGAACCCCCCTGGAGGGAGTGAGCACTGCATACCCAAGACTC TACAGTCAAAGCCTCAGTCTTTTGCCTTACCAGCGGCTACGAGAATCATGCTGCTTCCCACACCCATCTG TTTAGAACCAGATGTACAATATTCCATAGATGTCTATTTTTCTCAGCCTTTGCAAGGAGAGTCCCACGCT CATTCACATGTCCTGGTGGACTCTCTTGGCCTTATTCCCCAAATCAATTCATTGGAGAATTTCTGCAGCA AGCAGGACTTAGATGAGTATCAGCTTCACAACTGTGTTGAAATTGCCTCAGCAATGGGACCTCAAGTGCT CCCGGGTGCCTGTGAAAGGCTGATCATCAGCATGTCTGCCAAGCTGCATGATGGGGCTGTGGCCTGCAAG TGTCACCCCCAGGGCTCAGTCGGATCCAGCTGCAGCCGACTTGGAGGCCAGTGCCAGTGTAAACCTCTTG TGGTCGGGCGCTGCTGTGACAGGTGCTCAACTGGAAGCTATGATTTGGGGCATCACGGCTGTCACCCATG TCACTGCCATCCTCAAGGATCAAAGGACACTGTATGTGACCAAGTAACAGGACAGTGCCCCTGCCATGGA GAGGTGTCTGGCCGCCGCTGTGATCGCTGCCTGGCAGGCTACTTTGGATTTCCCAGCTGCCACCCTTGCC CTTGTAATAGGTTTGCTGAACTTTGTGATCCTGAGACAGGGTCATGCTTCAATTGTGGAGGCTTTACAAC TGGCAGAAACTGTGAAAGGTGTATTGATGGTTACTATGGAAATCCTTCTTCAGGACAGCCCTGTCGTCCT TGCCTGTGTCCAGATGATCCCTCAAGCAATCAGTATTTTGCCCATTCCTGTTATCAGAATCTGTGGAGCT CAGATGTAATCTGCAATTGTCTTCAAGGTTATACGGGTACTCAGTGTGGAGAATGCTCTACTGGTTTCTA TGGAAATCCAAGAATTTCAGGAGCACCTTGCCAACCATGTGCCTGCAACAACAACATAGATGTAACCGAT CCAGAGTCCTGCAGCCGGGTAACAGGGGAGTGCCTTCGATGTTTGCACAACACTCAGGGCGCAAACTGCC AGCTCTGCAAACCAGGTCACTATGGATCAGCCCTCAATCAGACCTGCAGAAGATGCTCCTGCCATGCTTC CGGCGTGAGTCCCATGGAGTGTCCCCCTGGTGGGGGAGCTTGCCTCTGTGACCCTGTCACTGGTGCATGT CCTTGTCTGCCGAATGTCACAGGCCTGGCCTGTGACCGTTGTGCTGATGGATACTGGAATCTGGTCCCTG GCAGAGGATGTCAGTCATGTGACTGTGACCCTAGGACCTCTCAAAGTAGCCACTGTGACCAGCTTACAGG CCAGTGTCCGTGTAAATTAGGTTACGGCGGGAAACGTTGCAGTGAGTGCCAGGAAAATTATTATGGTGAT CCACCTGGGCGATGCATTCCATGTGATTGTAACAGGGCAGGTACCCAGAAGCCCATCTGTGATCCAGACA CAGGCATGTGCCGCTGCCGGGAGGGTGTCAGCGGCCAGAGATGTGATCGCTGTGCCCGGGGACACAGCCA GGAATTCCCTACTTGTCTTCAATGTCACTTGTGCTTTGATCAGTGGGACCACACCATTTCTTCCCTCTCC AAAGCGGTGCAAGGGTTAATGAGACTGGCTGCTAACATGGAAGATAAAAGAGAGACCCTGCCTGTCTGTG AGGCAGACTTCAAAGACCTCAGAGGGAACGTGTCTGAAATAGAAAGGATTTTGAAACATCCTGTTTTCCC ATCTGGGAAATTCTTAAAAGTCAAGGATTATCATGACTCTGTTAGAAGACAAATCATGCAGCTAAATGAA CAACTGAAAGCAGTGTATGAATTTCAAGATCTGAAAGATACAATAGAAAGAGCAAAGAATGAAGCAGACC TCTTACTTGAAGACCTTCAGGAAGAAATTGATTTGCAATCCAGTGTCCTTAATGCAAGCATTGCGGACTC CTCAGAAAACATCAAGAAATATTATCACATATCATCATCTGCTGAAAAGAAAATTAATGAAACTAGTTCC ACCATTAATACCTCTGCAAATACAAGGAATGACTTACTTACCATCTTAGATACACTAACCTCAAAAGGAA ACTTGTCATTGGAAAGATTAAAGCAGATTAAGATACCAGATATCCAAATATTGAATGAAAAGGTGTGCGG AGATCCAGGAAATGTGCCATGTGTGCCCTTGCCCTGTGGCGGTGCTCTCTGCACGGGCCGGAAGGGGCAC AGGAAGTGTAGGGGTCCCGGCTGTCACGGCTCCCTGACCCTCTCAACGAATGCCCTCCAAAAAGCCCAGG AAGCAAAATCCATTATTCGTAATTTGGACAAACAGGTTCGTGGGTTGAAAAATCAGATCGAAAGTATAAG TGAACAGGCAGAAGTCTCCAAAAACAATGCCTTACAGCTGAGGGAAAAACTGGGAAATATAAGAAACCAA AGTGACTCTGAAGAAGAAAACATCAATCTTTTCATCAAAAAAGTGAAAAACTTTTTGTTAGAGGAAAACG TGCCTCCAGAAGACATCGAGAAGGTTGCGAATGGTGTGCTTGACATTCACCTACCAATTCCATCCCAAAA TCTAACCGATGAACTTGTCAAAATACAGAAACATATGCAACTCTGTGAGGATTACAGGACAGATGAAAAC AGGTTAAATGAAGAAGCAGATGGAGCCCAAAAGCTTTTGGTGAAGGCCAAAGCAGCTGAGAAAGCAGCAA ATATTCTATTAAATCTTGACAAAACATTGAACCAGTTACAACAAGCTCAAATCACTCAAGGACGGGCAAA CTCTACCATTACACAGCTGACTGCCAATATAACAAAAATAAAAAAGAATGTGCTGCAGGCTGAAAATCAA ACCAGGGAAATGAAGAGTGAGCTGGAGTTAGCAAAGCAGCGATCAGGGCTGGAGGATGGACTTTCCCTGC TGCAGACCAAGTTGCAAAGGCATCAAGACCACGCTGTCAATGCGAAAGTTCAGGCTGAATCTGCCCAACA CCAGGCTGGGAGTCTTGAGAAGGAATTTGTTGAGCTGAAAAAACAATATGCTATTCTCCAACGTAAGACA AGCACTACAGGACTAACAAAGGAGACATTAGGAAAAGTTAAACAGCTAAAAGATGCGGCAGAAAAATTGG CTGGAGATACAGAGGCCAAGATAAGAAGAATAACAGATTTAGAAAGGAAAATCCAAGATTTGAATCTAAG TAGACAAGCAAAAGCTGATCAACTGAGAATATTGGAAGATCAAGTTGTTGCCATTAAAAATGAAATTGTT GAACAAGAAAAAAAATATGCTAGGTGCTATAGC AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG215486 representing NM_007356
Red=Cloning site Green=Tags(s) MQFQLTLFLHLGWLSYSKAQDDCNRGACHPTTGDLLVGRNTQLMASSTCGLSRAQKYCILSYLEGEQKCS ICDSRFPYDPYDQPNSHTIENVTVSFEPDREKKWWQSENGLDHVSIRLDLEALFRFSHLILTFKTFRPAA MLVERSTDYGHNWKVFKYFAKDCATSFPNITSGQAQGVGDIVCDSKYSDIEPSTGGEVVLKVLDPSFEIE NPYSPYIQDLVTLTNLRINFTKLHTLGDALLGRRQNDSLDKYYYALYEMIVRGSCFCNGHASECRPMQKM RGDVFSPPGMVHGQCVCQHNTDGPNCERCKDFFQDAPWRPAADLQDNACRSCSCNSHSSRCHFDMTTYLA SGGLSGGVCEDCQHNTEGQHCDRCRPLFYRDPLKTISDPYACIPCECDPDGTISGGICVSHSDPALGSVA GQCLCKENVEGAKCDQCKPNHYGLSATDPLGCQPCDCNPLGSLPFLTCDVDTGQCLCLSYVTGAHCEECT VGYWGLGNHLHGCSPCDCDIGGAYSNVCSPKNGQCECRPHVTGRSCSEPAPGYFFAPLNFYLYEAEEATT LQGLAPLGSETFGQSPAVHVVLGEPVPGNPVTWTGPGFARVLPGAGLRFAVNNIPFPVDFTIAIHYETQS AADWTVQIVVNPPGGSEHCIPKTLQSKPQSFALPAATRIMLLPTPICLEPDVQYSIDVYFSQPLQGESHA HSHVLVDSLGLIPQINSLENFCSKQDLDEYQLHNCVEIASAMGPQVLPGACERLIISMSAKLHDGAVACK CHPQGSVGSSCSRLGGQCQCKPLVVGRCCDRCSTGSYDLGHHGCHPCHCHPQGSKDTVCDQVTGQCPCHG EVSGRRCDRCLAGYFGFPSCHPCPCNRFAELCDPETGSCFNCGGFTTGRNCERCIDGYYGNPSSGQPCRP CLCPDDPSSNQYFAHSCYQNLWSSDVICNCLQGYTGTQCGECSTGFYGNPRISGAPCQPCACNNNIDVTD PESCSRVTGECLRCLHNTQGANCQLCKPGHYGSALNQTCRRCSCHASGVSPMECPPGGGACLCDPVTGAC PCLPNVTGLACDRCADGYWNLVPGRGCQSCDCDPRTSQSSHCDQLTGQCPCKLGYGGKRCSECQENYYGD PPGRCIPCDCNRAGTQKPICDPDTGMCRCREGVSGQRCDRCARGHSQEFPTCLQCHLCFDQWDHTISSLS KAVQGLMRLAANMEDKRETLPVCEADFKDLRGNVSEIERILKHPVFPSGKFLKVKDYHDSVRRQIMQLNE QLKAVYEFQDLKDTIERAKNEADLLLEDLQEEIDLQSSVLNASIADSSENIKKYYHISSSAEKKINETSS TINTSANTRNDLLTILDTLTSKGNLSLERLKQIKIPDIQILNEKVCGDPGNVPCVPLPCGGALCTGRKGH RKCRGPGCHGSLTLSTNALQKAQEAKSIIRNLDKQVRGLKNQIESISEQAEVSKNNALQLREKLGNIRNQ SDSEEENINLFIKKVKNFLLEENVPPEDIEKVANGVLDIHLPIPSQNLTDELVKIQKHMQLCEDYRTDEN RLNEEADGAQKLLVKAKAAEKAANILLNLDKTLNQLQQAQITQGRANSTITQLTANITKIKKNVLQAENQ TREMKSELELAKQRSGLEDGLSLLQTKLQRHQDHAVNAKVQAESAQHQAGSLEKEFVELKKQYAILQRKT STTGLTKETLGKVKQLKDAAEKLAGDTEAKIRRITDLERKIQDLNLSRQAKADQLRILEDQVVAIKNEIV EQEKKYARCYS SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007356 |
| ORF Size | 5283 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007356.1, NP_031382.1 |
| RefSeq Size | 5874 bp |
| RefSeq ORF | 5286 bp |
| Locus ID | 22798 |
| UniProt ID | A4D0S4 |
| Protein Pathways | ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer |
| Gene Summary | Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.[UniProtKB/Swiss-Prot Function] |
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