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CACNA2D4 (NM_172364) Human Tagged ORF Clone
CAT＃: RG217052
- TrueORF®
CACNA2D4 (tGFP-tagged) - Human calcium channel, voltage-dependent, alpha 2/delta subunit 4 (CACNA2D4)
ORF Plasmid: DDK
"NM_172364" in other vectors (4)
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CNY 12,450.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | RCD4 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG217052 representing NM_172364
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCTGTGGCTGCTCTGCCCTCCTTCCCCTCCCCAACCCCAGGCCCACCATGCCTGCAACTCCCAACT TCCTCGCAAACCCCAGCTCCAGCAGCCGCTGGATTCCCCTCCAGCCAATGCCCGTGGCCTGGGCCTTTGT GCAGAAGACCTCGGCCCTCCTGTGGCTGCTGCTTCTAGGCACCTCCCTGTCCCCTGCGTGGGGACAGGCC AAGATTCCTCTGGAAACAGTGAAGCTATGGGCTGACACCTTCGGCGGGGACCTGTATAACACTGTGACCA AATACTCAGGCTCTCTCTTGCTGCAGAAGAAGTACAAGGATGTGGAGTCCAGTCTGAAGATCGAGGAGGT GGATGGCTTGGAGCTGGTGAGGAAGTTCTCAGAGGACATGGAGAACATGCTGCGGAGGAAAGTCGAGGCG GTCCAGAATCTGGTGGAAGCTGCCGAGGAGGCCGACCTGAACCACGAATTCAATGAATCCCTGGTGTTCG ACTATTACAACTCGGTCCTGATCAACGAGAGGGACGAGAAGGGCAACTTCGTGGAGCTGGGCGCCGAGTT CCTCCTGGAGTCCAATGCTCACTTCAGCAACCTGCCGGTGAACACCTCCATCAGCAGCGTGCAGCTGCCC ACCAACGTGTACAACAAAGACCCAGATATTTTAAATGGAGTCTACATGTCTGAAGCCTTGAATGCTGTCT TCGTGGAGAACTTCCAGAGAGACCCAACGTTGACCTGGCAATATTTTGGCAGTGCAACTGGATTCTTCAG GATCTATCCAGGTATAAAATGGACACCTGATGAGAATGGAGTCATTACTTTTGACTGCCGAAACCGCGGC TGGTACATTCAAGCTGCTACTTCTCCCAAGGACATAGTGATTTTGGTGGACGTGAGCGGCAGTATGAAGG GGCTGAGGATGACTATTGCCAAGCACACCATCACCACCATCTTGGACACCCTGGGGGAGAATGACTTCGT TAATATCATAGCGTACAATGACTACGTCCATTACATCGAGCCTTGTTTTAAAGGGATCCTCGTCCAGGCG GACCGAGACAATCGAGAGCATTTCAAACTGCTGGTGGAGGAGTTGATGGTCAAAGGTGTGGGGGTCGTGG ACCAAGCCCTGAGAGAAGCCTTCCAGATCCTGAAGCAGTTCCAAGAGGCCAAGCAAGGAAGCCTCTGCAA CCAGGCCATCATGCTCATCAGCGACGGCGCCGTGGAGGACTACGAGCCGGTGTTTGAGAAGTATAACTGG CCAGACTGTAAGGTCCGAGTTTTCACTTACCTCATTGGGAGAGAAGTGTCTTTTGCTGACCGCATGAAGT GGATTGCATGCAACAACAAAGGCTACTACACGCAGATCTCAACGCTGGCGGACACCCAGGAGAACGTGAT GGAATACCTGCACGTGCTCAGCCGCCCCATGGTCATCAACCACGACCACGACATCATCTGGACAGAGGCC TACATGGACAGCAAGCTCCTCAGCTCGCAGGCTCAGAGCCTGACACTGCTCACCACTGTGGCCATGCCAG TCTTCAGCAAGAAGAACGAAACGCGATCCCATGGCATTCTCCTGGGTGTGGTGGGCTCAGATGTGGCCCT GAGAGAGCTGATGAAGCTGGCGCCCCGGTACAAGCTTGGAGTGCACGGATACGCCTTTCTGAACACCAAC AATGGCTACATCCTCTCCCATCCCGACCTCCGGCCCCTGTACAGAGAGGGGAAGAAACTAAAACCCAAAC CTAACTACAACAGTGTGGATCTCTCCGAAGTGGAGTGGGAAGACCAGGCTGAATCTCTGAGAACAGCCAT GATCAATAGGGAAACAGGTACTCTCTCGATGGATGTGAAGGTTCCGATGGATAAAGGGAAGCGAGTTCTT TTCCTGACCAATGACTACTTCTTCACGGACATCAGCGACACCCCTTTCAGTTTGGGGGTGGTGCTGTCCC GGGGCCACGGAGAATACATCCTTCTGGGGAACACGTCTGTGGAAGAAGGCCTGCATGACTTGCTTCACCC AGACCTGGCCCTGGCCGGTGACTGGATCTACTGCATCACAGATATTGACCCAGACCACCGGAAGCTCAGC CAGCTAGAGGCCATGATCCGCTTCCTCACCAGGAAGGACCCAGACCTGGAGTGTGACGAGGAGCTGGTCC GGGAGGTGCTGTTTGACGCGGTGGTGACAGCCCCCATGGAAGCCTACTGGACAGCGCTGGCCCTCAACAT GTCCGAGGAGTCTGAACACGTGGTGGACATGGCCTTCCTGGGCACCCGGGCTGGCCTCCTGAGAAGCAGC TTGTTCGTGGGCTCCGAGAAGGTCTCCGACAGGAAGTTCCTGACACCTGAGGACGAGGCCAGCGTGTTCA CCCTGGACCGCTTCCCGCTGTGGTACCGCCAGGCCTCAGAGCATCCTGCTGGCAGCTTCGTCTTCAACCT CCGCTGGGCAGAAGGACCAGAAAGTGCGGGTGAACCCATGGTGGTGACGGCAAGCACAGCTGTGGCGGTG ACCGTGGACAAGAGGACAGCCATTGCTGCAGCCGCGGGCGTCCAAATGAAGCTGGAATTCCTCCAGCGCA AATTCTGGGCGGCAACGCGGCAGTGCAGCACTGTGGATGGGCCGTGCACACAGAGCTGCGAGGACAGTGA TCTGGACTGCTTCGTCATCGACAACAACGGGTTCATTCTGATCTCCAAGAGGTCCCGAGAGACGGGAAGA TTTCTGGGGGAGGTGGATGGTGCTGTCCTGACCCAGCTGCTCAGCATGGGGGTGTTCAGCCAAGTGACTA TGTATGACTATCAGGCCATGTGCAAACCCTCGAGTCACCACCACAGTGCAGCCCAGCCCCTGGTCAGCCC AATTTCTGCCTTCTTGACGGCGACCAGGTGGCTGCTGCAGGAGCTGGTGCTGTTCCTGCTGGAGTGGAGT GTCTGGGGCTCCTGGTACGACAGAGGGGCCGAGGCCAAAAGTGTCTTCCATCACTCCCACAAACACAAGA AGCAGGACCCGCTGCAGCCCTGCGACACGGAGTACCCCGTGTTCGTGTACCAGCCGGCCATCCGGGAGGC CAACGGGATCGTGGAGTGCGGGCCCTGCCAGAAGGTATTTGTGGTGCAGCAGATTCCCAACAGTAACCTC CTCCTCCTGGTGACAGACCCCACCTGTGACTGCAGCATCTTCCCACCAGTGCTGCAGGAGGCGACAGAAG TCAAATATAATGCCTCTGTCAAATGTGACCGGATGCGCTCCCAGAAGCTCCGCCGGCGACCAGACTCCTG CCACGCCTTCCATCCAGAGGAGAATGCCCAGGACTGCGGCGGCGCCTCGGACACCTCAGCCTCGCCGCCC CTACTCCTGCTGCCTGTGTGTGCCTGGGGGCTACTGCCCCAACTCCTGCGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG217052 representing NM_172364
Red=Cloning site Green=Tags(s) MVCGCSALLPLPNPRPTMPATPNFLANPSSSSRWIPLQPMPVAWAFVQKTSALLWLLLLGTSLSPAWGQA KIPLETVKLWADTFGGDLYNTVTKYSGSLLLQKKYKDVESSLKIEEVDGLELVRKFSEDMENMLRRKVEA VQNLVEAAEEADLNHEFNESLVFDYYNSVLINERDEKGNFVELGAEFLLESNAHFSNLPVNTSISSVQLP TNVYNKDPDILNGVYMSEALNAVFVENFQRDPTLTWQYFGSATGFFRIYPGIKWTPDENGVITFDCRNRG WYIQAATSPKDIVILVDVSGSMKGLRMTIAKHTITTILDTLGENDFVNIIAYNDYVHYIEPCFKGILVQA DRDNREHFKLLVEELMVKGVGVVDQALREAFQILKQFQEAKQGSLCNQAIMLISDGAVEDYEPVFEKYNW PDCKVRVFTYLIGREVSFADRMKWIACNNKGYYTQISTLADTQENVMEYLHVLSRPMVINHDHDIIWTEA YMDSKLLSSQAQSLTLLTTVAMPVFSKKNETRSHGILLGVVGSDVALRELMKLAPRYKLGVHGYAFLNTN NGYILSHPDLRPLYREGKKLKPKPNYNSVDLSEVEWEDQAESLRTAMINRETGTLSMDVKVPMDKGKRVL FLTNDYFFTDISDTPFSLGVVLSRGHGEYILLGNTSVEEGLHDLLHPDLALAGDWIYCITDIDPDHRKLS QLEAMIRFLTRKDPDLECDEELVREVLFDAVVTAPMEAYWTALALNMSEESEHVVDMAFLGTRAGLLRSS LFVGSEKVSDRKFLTPEDEASVFTLDRFPLWYRQASEHPAGSFVFNLRWAEGPESAGEPMVVTASTAVAV TVDKRTAIAAAAGVQMKLEFLQRKFWAATRQCSTVDGPCTQSCEDSDLDCFVIDNNGFILISKRSRETGR FLGEVDGAVLTQLLSMGVFSQVTMYDYQAMCKPSSHHHSAAQPLVSPISAFLTATRWLLQELVLFLLEWS VWGSWYDRGAEAKSVFHHSHKHKKQDPLQPCDTEYPVFVYQPAIREANGIVECGPCQKVFVVQQIPNSNL LLLVTDPTCDCSIFPPVLQEATEVKYNASVKCDRMRSQKLRRRPDSCHAFHPEENAQDCGGASDTSASPP LLLLPVCAWGLLPQLLR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_172364 |
| ORF Size | 3411 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_172364.3, NP_758952.3 |
| RefSeq Size | 5343 bp |
| RefSeq ORF | 3414 bp |
| Locus ID | 93589 |
| UniProt ID | Q7Z3S7 |
| Protein Families | Druggable Genome, Ion Channels: Other |
| Protein Pathways | Arrhythmogenic right ventricular cardiomyopathy (ARVC), Cardiac muscle contraction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway |
| Gene Summary | This gene encodes a member of the alpha-2/delta subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Various versions of each of these subunits exist, either expressed from similar genes or the result of alternative splicing. Research on a highly similar protein in rabbit suggests the protein described in this record is cleaved into alpha-2 and delta subunits. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217052 | CACNA2D4 (Myc-DDK-tagged)-Human calcium channel, voltage-dependent, alpha 2/delta subunit 4 (CACNA2D4) |
CNY 11,872.00 |
|
| RC217052L3 | Lenti ORF clone of Human calcium channel, voltage-dependent, alpha 2/delta subunit 4 (CACNA2D4), Myc-DDK-tagged |
CNY 13,210.00 |
|
| RC217052L4 | Lenti ORF clone of Human calcium channel, voltage-dependent, alpha 2/delta subunit 4 (CACNA2D4), mGFP tagged |
CNY 13,210.00 |
|
| SC311422 | CACNA2D4 (untagged)-Human calcium channel, voltage-dependent, alpha 2/delta subunit 4 (CACNA2D4) |
CNY 11,888.00 |
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