B3GAT2 (NM_080742) Human Tagged ORF Clone

CAT#: RG224329

  • TrueORF®

B3GAT2 (tGFP-tagged) - Human beta-1,3-glucuronyltransferase 2 (glucuronosyltransferase S) (B3GAT2)

ORF Plasmid: DDK tGFP



  "NM_080742" in other vectors (4)

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CNY 4,000.00

CNY 4,370.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms GLCATS
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG224329 representing NM_080742
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGTCCGCGCTTTTCACCCGCTTCTTTATCCTCCTGCCCTGGATCCTAATTGTCATCATCATGCTCG
ACGTGGACACGCGCAGGCCAGTGCCCCCGCTCACCCCGCGCCCCTACTTCTCTCCCTACGCGGTGGGCCG
CGGGGGCGCCCGACTCCCGCTCCGCAGGGGCGGCCCGGCTCACGGGACCCAAAAGCGCAACCAGTCTCGG
CCGCAGCCACAGCCGGAGCCGCAGCTGCCCACCATCTATGCCATCACGCCCACCTACAGCCGCCCGGTGC
AGAAAGCGGAGCTGACCCGCCTGGCCAACACGTTCCGCCAGGTGGCGCAGCTGCACTGGATCCTGGTGGA
GGACGCGGCGGCGCGCAGCGAGCTGGTGAGCCGCTTCCTGGCGCGGGCCGGGCTGCCCAGCACTCACCTG
CACGTGCCCACGCCGCGGCGCTACAAGCGGCCCGGGCTGCCGCGCGCCACTGAGCAGCGCAACGCGGGCC
TCGCCTGGCTGCGCCAGAGGCACCAGCACCAGCGCGCGCAGCCCGGCGTGCTCTTCTTCGCTGACGACGA
CAACACCTATAGTCTGGAGCTCTTCCAGGAGATGCGAACCACCCGCAAGGTCTCCGTCTGGCCTGTGGGC
CTGGTTGGTGGGCGGCGCTACGAACGTCCGCTGGTGGAAAACGGCAAAGTTGTTGGCTGGTACACCGGCT
GGAGAGCAGACAGGCCTTTTGCCATCGACATGGCAGGATTTGCTGTAAGTCTTCAAGTCATTTTGTCCAA
TCCAAAAGCTGTATTTAAGCGTCGTGGATCCCAGCCAGGGATGCAAGAATCTGACTTTCTCAAACAGATA
ACAACAGTCGAAGAACTGGAACCGAAAGCAAATAACTGCACTAAGGTTCTCGTGTGGCACACTCGGACAG
AGAAGGTTAATCTAGCCAACGAGCCAAAGTACCACCTGGACACAGTGAAAATTGAGGTA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG224329 representing NM_080742
Red=Cloning site Green=Tags(s)

MKSALFTRFFILLPWILIVIIMLDVDTRRPVPPLTPRPYFSPYAVGRGGARLPLRRGGPAHGTQKRNQSR
PQPQPEPQLPTIYAITPTYSRPVQKAELTRLANTFRQVAQLHWILVEDAAARSELVSRFLARAGLPSTHL
HVPTPRRYKRPGLPRATEQRNAGLAWLRQRHQHQRAQPGVLFFADDDNTYSLELFQEMRTTRKVSVWPVG
LVGGRRYERPLVENGKVVGWYTGWRADRPFAIDMAGFAVSLQVILSNPKAVFKRRGSQPGMQESDFLKQI
TTVEELEPKANNCTKVLVWHTRTEKVNLANEPKYHLDTVKIEV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_080742
ORF Size 969 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_080742.3
RefSeq Size 972 bp
RefSeq ORF 972 bp
Locus ID 135152
UniProt ID Q9NPZ5
Domains Glyco_transf_43
Protein Families Transmembrane
Protein Pathways Chondroitin sulfate biosynthesis, Heparan sulfate biosynthesis, Metabolic pathways
Gene Summary The product of this gene is a transmembrane protein belonging to the glucuronyltransferase family, and catalyzes the transfer of a beta-1,3 linked glucuronic acid to a terminal galactose in different glycoproteins or glycolipids containing a Gal-beta-1-4GlcNAc or Gal-beta-1-3GlcNAc residue. The encoded protein is involved in the synthesis of the human natural killer-1 (HNK-1) carbohydrate epitope, a sulfated trisaccharide implicated in cellular migration and adhesion in the nervous system. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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