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CaV1.3 (CACNA1D) (NM_001128840) Human Tagged ORF Clone
CAT＃: RG226473
- TrueORF®
CACNA1D (tGFP-tagged) - Human calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant 2
ORF Plasmid: DDK
"NM_001128840" in other vectors (2)
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CNY 20,900.00
货期*
12周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CACH3; CACN4; CACNL1A2; Cav1.3; CCHL1A2; PASNA; SANDD |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG226473 representing NM_001128840
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGATGATGATGATGATGAAAAAAATGCAGCATCAACGGCAGCAGCAAGCGGACCACGCGAACGAGG CAAACTATGCAAGAGGCACCAGACTTCCTCTTTCTGGTGAAGGACCAACTTCTCAGCCGAATAGCTCCAA GCAAACTGTCCTGTCTTGGCAAGCTGCAATCGATGCTGCTAGACAGGCCAAGGCTGCCCAAACTATGAGC ACCTCTGCACCCCCACCTGTAGGATCTCTCTCCCAAAGAAAACGTCAGCAATACGCCAAGAGCAAAAAAC AGGGTAACTCGTCCAACAGCCGACCTGCCCGCGCCCTTTTCTGTTTATCACTCAATAACCCCATCCGAAG AGCCTGCATTAGTATAGTGGAATGGAAACCATTTGACATATTTATATTATTGGCTATTTTTGCCAATTGT GTGGCCTTAGCTATTTACATCCCATTCCCTGAAGATGATTCTAATTCAACAAATCATAACTTGGAAAAAG TAGAATATGCCTTCCTGATTATTTTTACAGTCGAGACATTTTTGAAGATTATAGCGTATGGATTATTGCT ACATCCTAATGCTTATGTTAGGAATGGATGGAATTTACTGGATTTTGTTATAGTAATAGTAGGATTGTTT AGTGTAATTTTGGAACAATTAACCAAAGAAACAGAAGGCGGGAACCACTCAAGCGGCAAATCTGGAGGCT TTGATGTCAAAGCCCTCCGTGCCTTTCGAGTGTTGCGACCACTTCGACTAGTGTCAGGAGTGCCCAGTTT ACAAGTTGTCCTGAACTCCATTATAAAAGCCATGGTTCCCCTCCTTCACATAGCCCTTTTGGTATTATTT GTAATCATAATCTATGCTATTATAGGATTGGAACTTTTTATTGGAAAAATGCACAAAACATGTTTTTTTG CTGACTCAGATATCGTAGCTGAAGAGGACCCAGCTCCATGTGCGTTCTCAGGGAATGGACGCCAGTGTAC TGCCAATGGCACGGAATGTAGGAGTGGCTGGGTTGGCCCGAACGGAGGCATCACCAACTTTGATAACTTT GCCTTTGCCATGCTTACTGTGTTTCAGTGCATCACCATGGAGGGCTGGACAGATGTGCTCTACTGGATGA ATGATGCTATGGGATTTGAATTGCCCTGGGTGTATTTTGTCAGTCTCGTCATCTTTGGGTCATTTTTCGT ACTAAATCTTGTACTTGGTGTATTGAGCGGAGAATTCTCAAAGGAAAGAGAGAAGGCAAAAGCACGGGGA GATTTCCAGAAGCTCCGGGAGAAGCAGCAGCTGGAGGAGGATCTAAAGGGCTACTTGGATTGGATCACCC AAGCTGAGGACATCGATCCGGAGAATGAGGAAGAAGGAGGAGAGGAAGGCAAACGAAATACTAGCATGCC CACCAGCGAGACTGAGTCTGTGAACACAGAGAACGTCAGCGGTGAAGGCGAGAACCGAGGCTGCTGTGGA AGTCTCTGTCAAGCCATCTCAAAATCCAAACTCAGCCGACGCTGGCGTCGCTGGAACCGATTCAATCGCA GAAGATGTAGGGCCGCCGTGAAGTCTGTCACGTTTTACTGGCTGGTTATCGTCCTGGTGTTTCTGAACAC CTTAACCATTTCCTCTGAGCACTACAATCAGCCAGATTGGTTGACACAGATTCAAGATATTGCCAACAAA GTCCTCTTGGCTCTGTTCACCTGCGAGATGCTGGTAAAAATGTACAGCTTGGGCCTCCAAGCATATTTCG TCTCTCTTTTCAACCGGTTTGATTGCTTCGTGGTGTGTGGTGGAATCACTGAGACGATCTTGGTGGAACT GGAAATCATGTCTCCCCTGGGGATCTCTGTGTTTCGGTGTGTGCGCCTCTTAAGAATCTTCAAAGTGACC AGGCACTGGACTTCCCTGAGCAACTTAGTGGCATCCTTATTAAACTCCATGAAGTCCATCGCTTCGCTGT TGCTTCTGCTTTTTCTCTTCATTATCATCTTTTCCTTGCTTGGGATGCAGCTGTTTGGCGGCAAGTTTAA TTTTGATGAAACGCAAACCAAGCGGAGCACCTTTGACAATTTCCCTCAAGCACTTCTCACAGTGTTCCAG ATCCTGACAGGCGAAGACTGGAATGCTGTGATGTACGATGGCATCATGGCTTACGGGGGCCCATCCTCTT CAGGAATGATCGTCTGCATCTACTTCATCATCCTCTTCATTTGTGGTAACTATATTCTACTGAATGTCTT CTTGGCCATCGCTGTAGACAATTTGGCTGATGCTGAAAGTCTGAACACTGCTCAGAAAGAAGAAGCGGAA GAAAAGGAGAGGAAAAAGATTGCCAGAAAAGAGAGCCTAGAAAATAAAAAGAACAACAAACCAGAAGTCA ACCAGATAGCCAACAGTGACAACAAGGTTACAATTGATGACTATAGAGAAGAGGATGAAGACAAGGACCC CTATCCGCCTTGCGATGTGCCAGTAGGGGAAGAGGAAGAGGAAGAGGAGGAGGATGAACCTGAGGTTCCT GCCGGACCCCGTCCTCGAAGGATCTCGGAGTTGAACATGAAGGAAAAAATTGCCCCCATCCCTGAAGGGA GCGCTTTCTTCATTCTTAGCAAGACCAACCCGATCCGCGTAGGCTGCCACAAGCTCATCAACCACCACAT CTTCACCAACCTCATCCTTGTCTTCATCATGCTGAGCAGCGCTGCCCTGGCCGCAGAGGACCCCATCCGC AGCCACTCCTTCCGGAACACGATACTGGGTTACTTTGACTATGCCTTCACAGCCATCTTTACTGTTGAGA TCCTGTTGAAGATGACAACTTTTGGAGCTTTCCTCCACAAAGGGGCCTTCTGCAGGAACTACTTCAATTT GCTGGATATGCTGGTGGTTGGGGTGTCTCTGGTGTCATTTGGGATTCAATCCAGTGCCATCTCCGTTGTG AAGATTCTGAGGGTCTTAAGGGTCCTGCGTCCCCTCAGGGCCATCAACAGAGCAAAAGGACTTAAGCACG TGGTCCAGTGCGTCTTCGTGGCCATCCGGACCATCGGCAACATCATGATCGTCACCACCCTCCTGCAGTT CATGTTTGCCTGTATCGGGGTCCAGTTGTTCAAGGGGAAGTTCTATCGCTGTACGGATGAAGCCAAAAGT AACCCTGAAGAATGCAGGGGACTTTTCATCCTCTACAAGGATGGGGATGTTGACAGTCCTGTGGTCCGTG AACGGATCTGGCAAAACAGTGATTTCAACTTCGACAACGTCCTCTCTGCTATGATGGCGCTCTTCACAGT CTCCACGTTTGAGGGCTGGCCTGCGTTGCTGTATAAAGCCATCGACTCGAATGGAGAGAACATCGGCCCA ATCTACAACCACCGCGTGGAGATCTCCATCTTCTTCATCATCTACATCATCATTGTAGCTTTCTTCATGA TGAACATCTTTGTGGGCTTTGTCATCGTTACATTTCAGGAACAAGGAGAAAAAGAGTATAAGAACTGTGA GCTGGACAAAAATCAGCGTCAGTGTGTTGAATACGCCTTGAAAGCACGTCCCTTGCGGAGATACATCCCC AAAAACCCCTACCAGTACAAGTTCTGGTACGTGGTGAACTCTTCGCCTTTCGAATACATGATGTTTGTCC TCATCATGCTCAACACACTCTGCTTGGCCATGCAGCACTACGAGCAGTCCAAGATGTTCAATGATGCCAT GGACATTCTGAACATGGTCTTCACCGGGGTGTTCACCGTCGAGATGGTTTTGAAAGTCATCGCATTTAAG CCTAAGGGGTATTTTAGTGACGCCTGGAACACGTTTGACTCCCTCATCGTAATCGGCAGCATTATAGACG TGGCCCTCAGCGAAGCAGACCCAACTGAAAGTGAAAATGTCCCTGTCCCAACTGCTACACCTGGGAACTC TGAAGAGAGCAATAGAATCTCCATCACCTTTTTCCGTCTTTTCCGAGTGATGCGATTGGTGAAGCTTCTC AGCAGGGGGGAAGGCATCCGGACATTGCTGTGGACTTTTATTAAGTCCTTTCAGGCGCTCCCGTATGTGG CCCTCCTCATAGCCATGCTGTTCTTCATCTATGCGGTCATTGGCATGCAGATGTTTGGGAAAGTTGCCAT GAGAGATAACAACCAGATCAATAGGAACAATAACTTCCAGACGTTTCCCCAGGCGGTGCTGCTGCTCTTC AGGTGTGCAACAGGTGAGGCCTGGCAGGAGATCATGCTGGCCTGTCTCCCAGGGAAGCTCTGTGACCCTG AGTCAGATTACAACCCCGGGGAGGAGTATACATGTGGGAGCAACTTTGCCATTGTCTATTTCATCAGTTT TTACATGCTCTGTGCATTTCTGATCATCAATCTGTTTGTGGCTGTCATCATGGATAATTTCGACTATCTG ACCCGGGACTGGTCTATTTTGGGGCCTCACCATTTAGATGAATTCAAAAGAATATGGTCAGAATATGACC CTGAGGCAAAGGGAAGGATAAAACACCTTGATGTGGTCACTCTGCTTCGACGCATCCAGCCTCCCCTGGG GTTTGGGAAGTTATGTCCACACAGGGTAGCGTGCAAGAGATTAGTTGCCATGAACATGCCTCTCAACAGT GACGGGACAGTCATGTTTAATGCAACCCTGTTTGCTTTGGTTCGAACGGCTCTTAAGATCAAGACCGAAG GGAACCTGGAGCAAGCTAATGAAGAACTTCGGGCTGTGATAAAGAAAATTTGGAAGAAAACCAGCATGAA ATTACTTGACCAAGTTGTCCCTCCAGCTGGTGATGATGAGGTAACCGTGGGGAAGTTCTATGCCACTTTC CTGATACAGGACTACTTTAGGAAATTCAAGAAACGGAAAGAACAAGGACTGGTGGGAAAGTACCCTGCGA AGAACACCACAATTGCCCTACAGGCGGGATTAAGGACACTGCATGACATTGGGCCAGAAATCCGGCGTGC TATATCGTGTGATTTGCAAGATGACGAGCCTGAGGAAACAAAACGAGAAGAAGAAGATGATGTGTTCAAA AGAAATGGTGCCCTGCTTGGAAACCATGTCAATCATGTTAATAGTGATAGGAGAGATTCCCTTCAGCAGA CCAATACCACCCACCGTCCCCTGCATGTCCAAAGGCCTTCAATTCCACCTGCAAGTGATACTGAGAAACC GCTGTTTCCTCCAGCAGGAAATTCGGTGTGTCATAACCATCATAACCATAATTCCATAGGAAAGCAAGTT CCCACCTCAACAAATGCCAATCTCAATAATGCCAATATGTCCAAAGCTGCCCATGGAAAGCGGCCCAGCA TTGGGAACCTTGAGCATGTGTCTGAAAATGGGCATCATTCTTCCCACAAGCATGACCGGGAGCCTCAGAG AAGGTCCAGTGTGAAAAGAACCCGCTATTATGAAACTTACATTAGGTCCGACTCAGGAGATGAACAGCTC CCAACTATTTGCCGGGAAGACCCAGAGATACATGGCTATTTCAGGGACCCCCACTGCTTGGGGGAGCAGG AGTATTTCAGTAGTGAGGAATGCTACGAGGATGACAGCTCGCCCACCTGGAGCAGGCAAAACTATGGCTA CTACAGCAGATACCCAGGCAGAAACATCGACTCTGAGAGGCCCCGAGGCTACCATCATCCCCAAGGATTC TTGGAGGACGATGACTCGCCCGTTTGCTATGATTCACGGAGATCTCCAAGGAGACGCCTACTACCTCCCA CCCCAGCATCCCACCGGAGATCCTCCTTCAACTTTGAGTGCCTGCGCCGGCAGAGCAGCCAGGAAGAGGT CCCGTCGTCTCCCATCTTCCCCCATCGCACGGCCCTGCCTCTGCATCTAATGCAGCAACAGATCATGGCA GTTGCCGGCCTAGATTCAAGTAAAGCCCAGAAGTACTCACCGAGTCACTCGACCCGGTCGTGGGCCACCC CTCCAGCAACCCCTCCCTACCGGGACTGGACACCGTGCTACACCCCCCTGATCCAAGTGGAGCAGTCAGA GGCCCTGGACCAGGTGAACGGCAGCCTGCCGTCCCTGCACCGCAGCTCCTGGTACACAGACGAGCCCGAC ATCTCCTACCGGACTTTCACACCAGCCAGCCTGACTGTCCCCAGCAGCTTCCGGAACAAAAACAGCGACA AGCAGAGGAGTGCGGACAGCTTGGTGGAGGCAGTCCTGATATCCGAAGGCTTGGGACGCTATGCAAGGGA CCCAAAATTTGTGTCAGCAACAAAACACGAAATCGCTGATGCCTGTGACCTCACCATCGACGAGATGGAG AGTGCAGCCAGCACCCTGCTTAATGGGAACGTGCGTCCCCGAGCCAACGGGGATGTGGGCCCCCTCTCAC ACCGGCAGGACTATGAGCTACAGGACTTTGGTCCTGGCTACAGCGACGAAGAGCCAGACCCTGGGAGGGA TGAGGAGGACCTGGCGGATGAAATGATATGCATCACCACCTTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG226473 representing NM_001128840
Red=Cloning site Green=Tags(s) MMMMMMMKKMQHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMS TSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANC VALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLF SVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLF VIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNF AFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARG DFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGENRGCCG SLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANK VLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVT RHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQ ILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAE EKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVP AGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIR SHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVV KILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKS NPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENIGP IYNHRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIP KNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFK PKGYFSDAWNTFDSLIVIGSIIDVALSEADPTESENVPVPTATPGNSEESNRISITFFRLFRVMRLVKLL SRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLF RCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYL TRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNS DGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATF LIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEDDVFK RNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQV PTSTNANLNNANMSKAAHGKRPSIGNLEHVSENGHHSSHKHDREPQRRSSVKRTRYYETYIRSDSGDEQL PTICREDPEIHGYFRDPHCLGEQEYFSSEECYEDDSSPTWSRQNYGYYSRYPGRNIDSERPRGYHHPQGF LEDDDSPVCYDSRRSPRRRLLPPTPASHRRSSFNFECLRRQSSQEEVPSSPIFPHRTALPLHLMQQQIMA VAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYTPLIQVEQSEALDQVNGSLPSLHRSSWYTDEPD ISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEME SAASTLLNGNVRPRANGDVGPLSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001128840 |
| ORF Size | 6483 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001128840.3 |
| RefSeq Size | 7711 bp |
| RefSeq ORF | 6486 bp |
| Locus ID | 776 |
| UniProt ID | Q01668 |
| Protein Families | Druggable Genome, Ion Channels: Calcium, Transmembrane |
| Protein Pathways | Alzheimer's disease, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Calcium signaling pathway, Cardiac muscle contraction, Dilated cardiomyopathy, GnRH signaling pathway, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway, Type II diabetes mellitus, Vascular smooth muscle contraction |
| Gene Summary | Voltage-dependent calcium channels mediate the entry of calcium ions into excitable cells, and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, and gene expression. Calcium channels are multisubunit complexes composed of alpha-1, beta, alpha-2/delta, and gamma subunits. The channel activity is directed by the pore-forming alpha-1 subunit, whereas the others act as auxiliary subunits regulating this activity. The distinctive properties of the calcium channel types are related primarily to the expression of a variety of alpha-1 isoforms, namely alpha-1A, B, C, D, E, and S. This gene encodes the alpha-1D subunit. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226473 | CACNA1D (Myc-DDK-tagged)-Human calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant 2 |
CNY 18,990.00 |
|
| SC323309 | CACNA1D (untagged)-Human calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant 2 |
CNY 41,610.00 |
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