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Wfs1 (NM_031823) Rat Tagged ORF Clone
CAT＃: RR205185
- TrueORF®
Wfs1 (Myc-DDK-tagged ORF) - Rat Wolfram syndrome 1 homolog (human) (Wfs1), (10 ug)
"NM_031823" in other vectors (3)
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CNY 11,500.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR205185 representing NM_031823
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCTCAGGCACCCCACCTCCGAGCCCCTCTGGCCCACCTCCTCCACCCGCACCACAGCCCCAGGCCC GGGCCCGGCTCAATGCCACCACCTCACTGGAGCAGGACAAGATTGAACCGCCTCGTGCTCCTAGACCTCA GCCTGACTCCAGTGCTGGACGAAGTGCTGGGGAAGCAACCACGCCGGAGCCTCGGGCCCCTCACGCCAGC AGCCGAGAAGGGACAGATAAAGCTGGTCCCATGAAGGCAGATGTGGAGATCCCCTTTGAAGAAATCCTGG AGAAGGCCAAGGCTGGAGACCCCAAAGCACAGACAGAGGTGGGCAAACACTACCTGCGACTTGCCAATGA TGCAGATGAAGAGCTCAACAGCTGCTCGGCTGTGGCCTGGCTAATCCTGGCGGCCAAGCAGGGCAGGCGG GAGGCCGTGAAGCTGCTGAGGCGGTGCCTAGCTGACCGGAAAGGCATCACTTCTGAGAATGAGGCCGAGG TGAAGCAGCTATCCTCTGAGACCGACCTGGAAAGGGCAGTGCGCAAGGCTGCCCTGGTCATGTACTGGAA ACTCAACCCCAAGAAAAAGAAGCAGGTGGCTGTGTCTGAGCTGCTGGAGAATGTCGGGCAGGTCAACGAA CAGGATGGAGGGGCGCAGCCAGGCCCCGTCCCCAAGTCCCTGCAGAAGCAGAGGCGCATGCTGGAGCGTC TAGTGAGCAGTGAATCCAAGAACTACATTGCTCTGGACGATTTTGTGGAGCTCACCAAGAAGTATGCCAA GGGCATCATCCCCAACAACCTGTTCCTGCAGGATGAGGATGAAGATGAAGACGAGCTGTCAGGGAAGAGC CCCGAGGACCTGCCACTACGCCAGAAGGTGGTGAAGTACCCCTTACACGCCATCATGGAGATCAAAGAGT ACCTGATTGACGTGGCCTCCAAGGCAGGCATGCACTGGCTCTCCACCATCGTGCCCACCCATCACATCAA CGCCCTCATCTTCTTCTTCATCATCAGCAACCTAACCATCGACTTCTTCGCCTTCTTCATCCCCCTGGTG GTCTTCTACCTGTCCTTCGTGTCCATGGTCATCTGCACCCTCAAAGTGTTCCAGGACAGCAAGGCCTGGG AGAACTTCCGCACTCTCACCGACCTGCTGCTGCGCTTCGAGCCCAACCTAGATGTGGAGCAGGCGGAAGT GAACTTCGGCTGGAACCACCTGGAGCCTTACATCCACTTCCTGCTGTCAGTCGTCTTCGTGATCTTCTCC TTCCCGCTGGCCAGCAAGGACTGCATCCCGTGCTCCGAGCTCGCTGTCGTCTCCGCCTTCTTCACGGTGA CGAGCTACATGAGTCTGAGCAGCTCCGCCGAGCCCTACACCAGGCGGGCCCTGGTCACTGAGGTGGCTGC TGGGCTGCTGTCCCTTCTGCCCACCATGCCCGTGGACTGGCCCTTCCTGAAAGCACTCGGCCAGACGTTC TTCACCGTGCCCATCGGCCACTTCATCATCCTCAACGTCAGCCTCCCCTGCCTGCTCTATGTCTATCTCT TTTACCTCTTCTTCCGCATGGCCCAGCTGAGGAACTTCAAGGGCACCTACTGCTACCTGGTGCCCTACCT GGTGTGCTTCATGTGGTGTGAACTGTCCGTGGTCATCCTGCTCCAGTCCACCGGCCTGGGCTTGGTCCGT GCCTCCATCGGCTACTTCCTCTTCCTCTTTGCCCTCCCCATCCTGGTGGCTGGCCTTGCCCTCATGGGCA CCGTGCAGTTTGCCCGATGGTTCCTGTCACTGGACCTCACCAAGATCATGGTCACCACAGTGATCTGCAG CGTGCCCCTGCTTTTCCGCTGGTGGACCAAGGCCAACTTTTCGGTGGTGGGAATGGTCAAGTCCCTGACT CGGAGCTCCATAGTGAAGCTCATTCTGGTGTGGCTCACGGCCATCCTGCTCTTTTGCTGGTTCTACGTGT ACCGGTCGGAAGGCATGAAGGTCTACAATTCCACACTCACCTGGCAGCAATATGGCTTCCTGTGTGGGCC ACGGGCCTGGAAGGAGACTAACATGGCCCGGACCCAGATCCTGTGCAGCCACCTGGAGGGCCACAGGGTC ACGTGGACAGGCCGCTTCAAGTATGTCCGAGTGACCGAGATCGACAACAGCGCCGAGTCGGCCATCAACA TGCTCCCGTTCTTCCTGGGTGACTGGATGCGCTGCCTGTACGGTGAGGCCTACCCATCCTGTAGCTCTGG TAACACGTCCACGGCTGAGGAGGAGCTCTGCCGTCTCAAGCAGCTGGCCAAGCACCCCTGCCACATCAAG AAGTTTGACCGCTACAAGTTTGAGATCACAGTGGGCATGCCCTTCGGCACCAACGGCAACCGCGGCCACG AAGAAGACGACATCACCAAGGACATCGTCCTGCGAGCCAGCAGCGAGTTCAAGGACGTGCTGCTGAACCT GCGTCAGGGCAGCCTCATAGAGTTCAGCACCATCCTCGAGGGCCGCCTGGGTAGCAAGTGGCCCGTCTTC GAGCTCAAGGCCATCAGCTGCCTCAATTGCATGACACAGCTGTCCCCTGCCCGGAGGCACGTAAAGATCG AACAGGACTGGCGTAGCACGGTGCACGGTGCCCTCAAGTTCGCCTTCGACTTCTTCTTCTTCCCATTCCT GTCTGCCGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR205185 representing NM_031823
Red=Cloning site Green=Tags(s) MSSGTPPPSPSGPPPPPAPQPQARARLNATTSLEQDKIEPPRAPRPQPDSSAGRSAGEATTPEPRAPHAS SREGTDKAGPMKADVEIPFEEILEKAKAGDPKAQTEVGKHYLRLANDADEELNSCSAVAWLILAAKQGRR EAVKLLRRCLADRKGITSENEAEVKQLSSETDLERAVRKAALVMYWKLNPKKKKQVAVSELLENVGQVNE QDGGAQPGPVPKSLQKQRRMLERLVSSESKNYIALDDFVELTKKYAKGIIPNNLFLQDEDEDEDELSGKS PEDLPLRQKVVKYPLHAIMEIKEYLIDVASKAGMHWLSTIVPTHHINALIFFFIISNLTIDFFAFFIPLV VFYLSFVSMVICTLKVFQDSKAWENFRTLTDLLLRFEPNLDVEQAEVNFGWNHLEPYIHFLLSVVFVIFS FPLASKDCIPCSELAVVSAFFTVTSYMSLSSSAEPYTRRALVTEVAAGLLSLLPTMPVDWPFLKALGQTF FTVPIGHFIILNVSLPCLLYVYLFYLFFRMAQLRNFKGTYCYLVPYLVCFMWCELSVVILLQSTGLGLVR ASIGYFLFLFALPILVAGLALMGTVQFARWFLSLDLTKIMVTTVICSVPLLFRWWTKANFSVVGMVKSLT RSSIVKLILVWLTAILLFCWFYVYRSEGMKVYNSTLTWQQYGFLCGPRAWKETNMARTQILCSHLEGHRV TWTGRFKYVRVTEIDNSAESAINMLPFFLGDWMRCLYGEAYPSCSSGNTSTAEEELCRLKQLAKHPCHIK KFDRYKFEITVGMPFGTNGNRGHEEDDITKDIVLRASSEFKDVLLNLRQGSLIEFSTILEGRLGSKWPVF ELKAISCLNCMTQLSPARRHVKIEQDWRSTVHGALKFAFDFFFFPFLSAA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_031823 |
| ORF Size | 2670 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_031823.1, NP_114011.1 |
| RefSeq Size | 2742 bp |
| RefSeq ORF | 2673 bp |
| Locus ID | 83725 |
| MW | 100.5 kDa |
| Gene Summary | endoplasmic reticulum membrane glycoprotein; postulated to be involved in neuronal membrane trafficking or protein processing [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN205185 | Wfs1 (untagged ORF) - Rat Wolfram syndrome 1 homolog (human) (Wfs1), (10 ug) |
CNY 11,500.00 |
|
| RR205185L3 | Lenti ORF clone of Wfs1 (Myc-DDK-tagged ORF) - Rat Wolfram syndrome 1 homolog (human) (Wfs1), (10 ug) |
CNY 13,210.00 |
|
| RR205185L4 | Lenti ORF clone of Wfs1 (mGFP-tagged ORF) - Rat Wolfram syndrome 1 homolog (human) (Wfs1), (10 ug) |
CNY 14,350.00 |
