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Vars2 (NM_213563) Rat Tagged ORF Clone
CAT＃: RR206349
- TrueORF®
Vars2 (Myc-DDK-tagged ORF) - Rat valyl-tRNA synthetase 2, mitochondrial (putative) (Vars2), nuclear gene encoding mitochondrial protein, (10 ug)
"NM_213563" in other vectors (3)
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CNY 17,100.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Vars2l; Varsl |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR206349 representing NM_213563
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCATTTACCTCTGGCCTCTTTCCGGCCACCTCTCTGGGGGCTGAGGCCCTCATGGGGTCTCGCCA GGCCCCGGGCACTTTGTACACAACCGGAGCCCCACGGCTCCCCAGTCTCTCGGAGGAACCGTGAAGCCAA ACAGAAGCGCCTGCGGGAGAAGCAGGCGGCCCTGGAGGCTGGGCTGGCTGAGAAGAGCAAGACACCTGCA GTGCCCACTAAGGCCTGGAGTCACAAGGAAGTAGTATTGTATGAAATCCCCACCCGGCCAGGTGAAAAGA AAGATGTTTCCGGGCCCCTGCCTCCTGCTTACAGCCCCCAATATGTTGAAGCAGCCTGGTACCAGTGGTG GGTGCGAGAGGGCTTCTTCAAACCAGAGTATCAGGCAAGGCTGCCCCAAGCTACAGGGGAGACCTTTTCC ATGTGTATCCCACCTCCCAATGTCACGGGCTCCCTGCACATTGGCCATGCGCTCACAGTGGCGATACAGG ATGCTTTTGTGCGCTGGCACCGGATGCGTGGGGATCGTGTGCTGTGGATCCCCGGGTCAGATCATGCAGG AATTGCTACACAGGCTGTGGTGGAGAAACAGCTGTGGAGGGAGCGGAGAGTGCGGAGACACGAGCTGAGC CGGGAGGACTTCCTCAGGGCTGTGTGGCAGTGGAAACAGGAGAAAGGAGGGGAGATCTACGAGCAGCTGT GCGCCCTGGGCGCCTCTCTGGACTGGGATCGAGAGTGTTTCACCATGGATGCCGGCTCCTCAGCAGCTGT GACGGAAGCTTTTGTGCGACTCTATGACCTGGGATTGTTGTACCGGAACCGCCAGCTTGTCAACTGGTCA TGTACTCTGAGATCGGCCATCTCAGACATTGAGGTAGAAAGCCGGCCCCTACCTGGCCGCACGGTGTTGC GGCTGCCCGGCTGCCCAATCCCCGTGTCTTTTGGGCTCCTGGTTTCCATTGCTTTCCCTGTGGATGGAGA CCCTGGCACAGAGATTGTGGTGGGAACCACCAGGCCAGAGACCCTGCCTGGAGATGTGGCTGTGGCCGTT CACCCAGATGACCCAAGATACACGCATTTACATGGGCGCCAGCTCCGTCACCCTTTGACAGGACAGCATC TCCCCCTCATCACAGACACCACTGTTCAGCCACATGTGGGTACAGGGGCGGTGAAGGTGACTCCTGCTCA CAGTCCCGCAGACGCTGAGATGGGGACCCGGCATGGCTTGACCCCACTGAGTGTCATTGCAGAGGATGGG ACCATGACGTCCCTCTGTGGAGACTGGCTGCAGGGCCTTCACCGATTCGTAGCCCGGGAAAAGATCATGT GCACACTGAGGGAGCGGGGCCTGTTCCGGGGCCTACAGGAGCACCCCATGGTGCTGCCCATCTGCAGCCG TTCCGGGGATGTCGTGGAGTACCTGCTGAAGAGCCAGTGGTTTGTGCGCTGCCAGGAAATGGGAGACCGA GCTGCCAAGGCTGTGGAGTCGGGGGCTCTGGAGCTCTGGCCCTCCTTCCACCAGAAGAGCTGGCAACACT GGTTTGCCCACATTGGGGACTGGTGTGTCTCCCGGCAGCTGTGGTGGGGCCATCAGATTCCAGCCTACCG GGTCGGCGGGGAGAAAGCAGAGGATGACAGGGAGGAATGCTGGGTGGTTGGACGGTCAGAGGCTGAGGCC AGAGCAGCAGCAGCAAAACAGACAGGAAGACCAGAGGCAGAGCTGACCCTGGAGAGGGACCCTGATGTCC TGGACACTTGGTTCTCTTCAGCTCTTTTCCCCTTCTCTGCCTTGGGTTGGCCCCAAGAGACGCCAGACCT TGCTCGCTTCTACCCCCTCACACTTCTGGAAACGGGCAGTGACCTCCTGACGTTCTGGGTGGGCCGCATG GTCATGCTGGGGACCCAGCTCACTGGACAGCTCCCCTTCAGCAAGGTGCTCCTTCACTCCATGGTTCGGG ACAGGCAGGGCCGGAAGATGAGCAAGTCCCTGGGGAACGTGCTAGACCCGCGGGACATCATCAGTGGGCA GGAGCTGCAGGTGCTCCAGGCCAAGCTGAGAGATGGCAACTTGGACCAGGGAGAGCTCGCTGTTGCAGCC GCAGCACAGAAAAAGGACTTTCCTTACGGGATCCCCGAGTGTGGGACGGATGCCCTGAGATTTGCACTGT GCTCCCATGGAATCCTGGGTGGCGACCTGCACCTGTCGGTCTCAGAGGTCCTGAACTACCGCCATTTCTG CAACAAGCTCTGGAATGCCCTGCGCTTTATCCTCCGTGCCCTGGGGGATGATTTCGTGCCCCAGCCCGCA GAGAAGGTAACGCCCTCCTCCCCCATGGACGCCTGGATTCTGAGCCGCTTGGCCTTTGCGGCCAACGAGT GTGAGAGGGGCTTCCTCAGCCGGGAGCTTTCGCTTGTCACCCACACCCTGTATCATTTCTGGCTCCACAA TCTCTGTGATGTCTACCTGGAAGCGGTGAAGCCAGTGTTGTCGAGTGCGCCCTGTCCCCCAGGACCCCCT CAGGTCCTGTTCTCCTGTGCAGATGTCGGTCTGCGCCTCCTCGCCCCGCTGATGCCCTTCCTGGCAGAAG AACTTTGGCAGAGGCTGCCCCCCAGGCAAGGTGGCTCCATGGCTCCCAGCATCTGTGTGGCCCCCTACCC CAGTGGTCACAGCCTGGTGAGTCGTGGGCAGGAGTCCTGGCGCCAGCCAGAGTTGGAGCACTGCTTCTCC CGAGTCCAAGAGATTGTACAGGCACTGCGGGCGCTTCGAGCCACCTACCAGCTCACAAGAGCCCGACCCC ATGTGCTGCTACAGAGCTCAGACCCAGGAGAACAAGGCCTAGTCCAGCCCTTCCTGGAACCTCTGGGCGT CCTGAGCCACTGTGGGGCTGTAGGATTCCTGCCCCCAGGAGCAGCAGCTCCCTCAGGGTGGGCCCTGGCC CCACTGGGTGACACCATAAAGATCTACATGGAGCTGCAGGGCCTGGTGGACCCCCAGAGCCAGCTACCTC GGCTAGCTGCCCGGAGGCAGAAGTTACAGAAGCAGCTTGATGACCTCTTGAACCGGACCGTGTCCGATGG TCCTGCAGAGAGGCAGCAGAGGATTTCCTCCCTCCAGCTGGAATTGTCAAAGCTGGACCAGGCAGCCTCT CACCTACAGCAACTGATGGAGGAGGCTCCAGACGCCAGGGAGCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR206349 representing NM_213563
Red=Cloning site Green=Tags(s) MPHLPLASFRPPLWGLRPSWGLARPRALCTQPEPHGSPVSRRNREAKQKRLREKQAALEAGLAEKSKTPA VPTKAWSHKEVVLYEIPTRPGEKKDVSGPLPPAYSPQYVEAAWYQWWVREGFFKPEYQARLPQATGETFS MCIPPPNVTGSLHIGHALTVAIQDAFVRWHRMRGDRVLWIPGSDHAGIATQAVVEKQLWRERRVRRHELS REDFLRAVWQWKQEKGGEIYEQLCALGASLDWDRECFTMDAGSSAAVTEAFVRLYDLGLLYRNRQLVNWS CTLRSAISDIEVESRPLPGRTVLRLPGCPIPVSFGLLVSIAFPVDGDPGTEIVVGTTRPETLPGDVAVAV HPDDPRYTHLHGRQLRHPLTGQHLPLITDTTVQPHVGTGAVKVTPAHSPADAEMGTRHGLTPLSVIAEDG TMTSLCGDWLQGLHRFVAREKIMCTLRERGLFRGLQEHPMVLPICSRSGDVVEYLLKSQWFVRCQEMGDR AAKAVESGALELWPSFHQKSWQHWFAHIGDWCVSRQLWWGHQIPAYRVGGEKAEDDREECWVVGRSEAEA RAAAAKQTGRPEAELTLERDPDVLDTWFSSALFPFSALGWPQETPDLARFYPLTLLETGSDLLTFWVGRM VMLGTQLTGQLPFSKVLLHSMVRDRQGRKMSKSLGNVLDPRDIISGQELQVLQAKLRDGNLDQGELAVAA AAQKKDFPYGIPECGTDALRFALCSHGILGGDLHLSVSEVLNYRHFCNKLWNALRFILRALGDDFVPQPA EKVTPSSPMDAWILSRLAFAANECERGFLSRELSLVTHTLYHFWLHNLCDVYLEAVKPVLSSAPCPPGPP QVLFSCADVGLRLLAPLMPFLAEELWQRLPPRQGGSMAPSICVAPYPSGHSLVSRGQESWRQPELEHCFS RVQEIVQALRALRATYQLTRARPHVLLQSSDPGEQGLVQPFLEPLGVLSHCGAVGFLPPGAAAPSGWALA PLGDTIKIYMELQGLVDPQSQLPRLAARRQKLQKQLDDLLNRTVSDGPAERQQRISSLQLELSKLDQAAS HLQQLMEEAPDAREL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_213563 |
| ORF Size | 3195 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_213563.1, NP_998728.1 |
| RefSeq Size | 4099 bp |
| RefSeq ORF | 3198 bp |
| Locus ID | 309596 |
| UniProt ID | Q6MG21 |
| MW | 118.9 kDa |
| Gene Summary | may act as a valine-tRNA ligase in protein synthesis [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN206349 | Vars2 (untagged ORF) - Rat valyl-tRNA synthetase 2, mitochondrial (putative) (Vars2), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 17,100.00 |
|
| RR206349L3 | Lenti ORF clone of Vars2 (Myc-DDK-tagged ORF) - Rat valyl-tRNA synthetase 2, mitochondrial (putative) (Vars2), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 18,720.00 |
|
| RR206349L4 | Lenti ORF clone of Vars2 (mGFP-tagged ORF) - Rat valyl-tRNA synthetase 2, mitochondrial (putative) (Vars2), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 20,520.00 |
