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HARS2 (NM_012208) Human Untagged Clone
CAT＃: SC102514
HARS2 (untagged)-Human histidyl-tRNA synthetase 2, mitochondrial (putative) (HARS2), nuclear gene encoding mitochondrial protein
CNY 2,950.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HARSL; HARSR; HisRS; HO3; PRLTS2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC102514 sequence for NM_012208 edited (data generated by NextGen Sequencing)
ATGCCCCTGCTCGGACTTCTTCCCAGGAGGGCCTGGGCTTCGCTGCTCAGCCAGCTCCTG CGACCGCCCTGCGCTTCGTGCACCGGGGCGGTCCGTTGCCAAAGCCAGGTTGCAGAGGCA GTGTTAACATCCCAACTGAAAGCACATCAAGAGAAACCAAATTTTATTATCAAGACCCCA AAGGGTACCAGGGATCTTAGTCCTCAGCATATGGTTGTGAGGGAGAAAATTCTTGATTTG GTTATCAGCTGCTTTAAACGTCATGGAGCAAAGGGGATGGACACCCCAGCATTTGAGCTG AAGGAAACCCTGACTGAGAAGTATGGGGAGGACTCTGGGCTCATGTATGATCTGAAGGAT CAAGGTGGAGAGCTGTTGTCCCTCCGCTATGACCTTACTGTTCCCTTTGCTCGTTATCTG GCCATGAATAAGGTGAAGAAGATGAAACGTTATCATGTTGGAAAGGTGTGGCGGCGAGAG AGCCCAACCATAGTCCAAGGCCGTTATAGGGAGTTCTGCCAGTGTGATTTTGACATTGCT GGTCAGTTTGACCCTATGATCCCCGATGCAGAGTGTTTGAAGATCATGTGTGAAATCCTA AGTGGATTGCAGTTGGGAGACTTTCTCATTAAGGTAAATGACCGGCGGATTGTGGATGGG ATGTTTGCTGTCTGTGGTGTTCCTGAAAGCAAGTTCCGTGCCATCTGCTCCTCCATAGAT AAACTAGACAAGATGGCTTGGAAAGATGTGAGACATGAGATGGTGGTGAAGAAAGGCCTG GCTCCTGAGGTGGCTGATCGAATTGGGGACTATGTCCAGTGTCATGGTGGGGTATCCCTA GTAGAGCAAATGTTTCAGGATCCCAGACTATCCCAGAACAAGCAGGCCCTGGAGGGCCTG GGAGACCTAAAGCTGCTATTTGAATACCTGACTTTATTTGGAATTGCTGATAAGATCTCC TTTGACCTCAGCCTGGCTCGGGGCCTAGACTACTATACAGGAGTGATCTATGAAGCAGTG CTGCTGCAGACCCCAACTCAGGCTGGGGAGGAGCCCCTGAATGTGGGCAGTGTGGCTGCT GGTGGGCGCTATGATGGGCTGGTGGGCATGTTTGACCCCAAGGGCCACAAGGTGCCATGT GTGGGACTCAGCATTGGGGTTGAGCGAATCTTCTACATTGTGGAGCAGAGGATGAAGACC AAAGGTGAGAAGGTGCGGACTACAGAGACTCAAGTGTTTGTGGCCACACCACAGAAGAAC TTTCTCCAAGAACGGTTGAAGCTTATTGCAGAGCTTTGGGATTCTGGAATCAAGGCAGAG ATGCTATACAAGAACAACCCCAAACTATTAACCCAGCTGCACTATTGTGAGAGCACAGGC ATTCCACTGGTGGTCATTATTGGTGAGCAAGAACTGAAAGAAGGGGTCATCAAGATCCGT TCAGTGGCCAGCAGAGAGGAGGTGGCCATTAAACGGGAAAATTTTGTGGCTGAAATTCAG AAGCGACTGTCTGAGTCTTGA Clone variation with respect to NM_012208.2 327 a=>g >OriGene 5' read for NM_012208 unedited
GCATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGGCTACTA AGGGAACTTGGGAGGATCCCACCTCAGCCTTCGTGACTAGTGAGGTGCGCAAACGCCCGA GTTTTCCCTGGTGCGCGGGTTCCGCCTTTGCAGTGCCCTCCACCCTTCCTGGTGTCTGAC CCGCCTCCTTCCCAGGCCTTTTGTTCCTGTCCCGGAAAGCCGGCGTCCTGCCGCGCGATG CCCCTGCTCGGACTTCTTCCCAGGAGGGCCTGGGCTTCGCTGCTCAGCCAGCTCCTGCGA CCGCCCTGCGCTTCGTGCACCGGGGCGGTCCGTTGCCAAAGCCAGGTTGCAGAGGCAGTG TTAACATCCCAACTGAAAGCACATCAAGAGAAACCAAATTTTATTATCAAGACCCCAAAG GGTACCAGGGATCTTAGTCCTCAGCATATGGTTGTGAGGGAGAAAATTCTTGATTTGGTT ATCAGCTGCTTTAAACGTCATGGAGCAAAGGGGATGGACACCCCAGCATTTGAGCTGAAG GAAACCCTGACTGAGAAGTATGGGGAGGACTCTGGGCTCATGTATGATCTGAAGGATCAA GGTGGAGAGCTGTTGTCCCTCCGCTATGACCTTACTGTTCCCTTTGCTCGTTATCTGGCC ATGAATAAGGTGAAGAAGATGAAACGTTATCATGTTGGAAAGGTGTGGCGGCGAGAGAGC CCAACCATAGTCCAAGGCCGTTATAGGGAGTTCTGCCAGTGTGATTNTGACATTGCTGGT CAGTTTGACCCTATGATCCCCGATGCAGAGTGGTTGAAGATCATGTGTGAAATCCTAAGT GGATTGCAGTTGGGAGACTNTCTCATTAANGGTAATGACCNGCNGATNGTGNATGGGGAT GTTGCTGTCTTGTGTGTCCTGGAAAGCNAGTNCCGTGCCATCTGCTCTCCTAGATAACTA GACAGATGCTGGGAAGATG >OriGene 3' read for NM_012208 unedited
NAAATTTTTGACTNTAGNCCCGCGGNCCCGCTTTCNANNGATCGGTTTTTTTTTTTTTTT TTTGCACTTAATATTTTATTTAACATGTTATCCAACCACTTTCTAAAACAGCCTCCATGG CACGGTATGGAATAGAGCAGTGATTCTGGCACAGGAAATGGTCTCCAAAATCTCAAAATA TCAGATCTCTACCTTGTCAAGTCATCTATTAAGTTCAATGTTCAAGATTATCAAGCATAC AGCCCCACTCTTCTACTGTAAGCAATGGATGGGCTAGAGAGATGTCAAATCAACATGGGT ACTGCCATGCCCTTAGGATAGCATCTGTCCTCAAAGCTGAAAGCAGCCATTGCCCCTCTT TGCTGTCTTCCAAAAACTCCCCAGACAGGCCAATGGCTGATTGTGGACAAATTCACCAGA ATCTGGTTCCGTAGCTACAGTAGAAGGCTAAGTATCTGGTTTCTGATCTCAATGGTTCAG AATCCTGAGCCTGAGGTGACTCTCCCAGAGCCTTGCTCACAGCAACAATCTCACTACCTC TTGACAACGTTCCGTGGCACTTCAAATTTGCCTCTGCAGCTAGAGCATGTCTCTTTCACA TCCAGCCATCTGCACCCATGCAGAATAGCTGCTCACACTAGCCCAGGAGTCCTCAGGGTC TTCTACAGTTCTGCACCCAGGAAGAATGGAGGAGGCAGAAGGTACTTGTCACCCTTCCTG GCTAGTTGTTGTGAAGTCCATCACTCAATTCCAGAGGAATTCAGTTCTAGAGAAAACCTT TTCTACAAAGAGCAGCAGATGGGAATCANGCAAGGATCAAGACTCAGACAGTCGCTTCTG AATTTCAGCCACAAAATTTNCCCGTTAATGGCCCACTNCTCTCTGCTGGCCACTGNAACG GAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_012208 |
| Insert Size | 2550 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012208.2, NP_036340.1 |
| RefSeq Size | 2508 bp |
| RefSeq ORF | 1521 bp |
| Locus ID | 23438 |
| UniProt ID | P49590 |
| Domains | tRNA-synt_2b, HGTP_anticodon |
| Protein Pathways | Aminoacyl-tRNA biosynthesis |
| Gene Summary | Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is an enzyme belonging to the class II family of aminoacyl-tRNA synthetases. Functioning in the synthesis of histidyl-transfer RNA, the enzyme plays an accessory role in the regulation of protein biosynthesis. The gene is located in a head-to-head orientation with HARS on chromosome five, where the homologous genes likely share a bidirectional promoter. Mutations in this gene are associated with the pathogenesis of Perrault syndrome, which involves ovarian dysgenesis and sensorineural hearing loss. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204925 | HARS2 (Myc-DDK-tagged)-Human histidyl-tRNA synthetase 2, mitochondrial (putative) (HARS2), nuclear gene encoding mitochondrial protein |
CNY 3,768.00 |
|
| RC204925L3 | Lenti ORF clone of Human histidyl-tRNA synthetase 2, mitochondrial (putative) (HARS2), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204925L4 | Lenti ORF clone of Human histidyl-tRNA synthetase 2, mitochondrial (putative) (HARS2), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5,890.00 |
|
| RG204925 | HARS2 (tGFP-tagged) - Human histidyl-tRNA synthetase 2, mitochondrial (putative) (HARS2), nuclear gene encoding mitochondrial protein |
CNY 4,370.00 |
