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BAP1 (NM_004656) Human Untagged Clone
CAT＃: SC117256
BAP1 (untagged)-Human BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase) (BAP1)
CNY 8,016.00
| Cited in 4 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | hucep-6; HUCEP-13; UCHL2 |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>NCBI ORF sequence for NM_004656, the custom clone sequence may differ by one or more nucleotides
ATGAATAAGGGCTGGCTGGAGCTGGAGAGCGACCCAGGCCTCTTCACCCTGCTCGTGGAAGATTTCGGTG TCAAGGGGGTGCAAGTGGAGGAGATCTACGACCTTCAGAGCAAATGTCAGGGCCCTGTATATGGATTTAT CTTCCTGTTCAAATGGATCGAAGAGCGCCGGTCCCGGCGAAAGGTCTCTACCTTGGTGGATGATACGTCC GTGATTGATGATGATATTGTGAATAACATGTTCTTTGCCCACCAGCTGATACCCAACTCTTGTGCAACTC ATGCCTTGCTGAGCGTGCTCCTGAACTGCAGCAGCGTGGACCTGGGACCCACCCTGAGTCGCATGAAGGA CTTCACCAAGGGTTTCAGCCCTGAGAGCAAAGGATATGCGATTGGCAATGCCCCGGAGTTGGCCAAGGCC CATAATAGCCATGCCAGGCCCGAGCCACGCCACCTCCCTGAGAAGCAGAATGGCCTTAGTGCAGTGCGGA CCATGGAGGCGTTCCACTTTGTCAGCTATGTGCCTATCACAGGCCGGCTCTTTGAGCTGGATGGGCTGAA GGTCTACCCCATTGACCATGGGCCCTGGGGGGAGGACGAGGAGTGGACAGACAAGGCCCGGCGGGTCATC ATGGAGCGTATCGGCCTCGCCACTGCAGGGGAGCCCTACCACGACATCCGCTTCAACCTGATGGCAGTGG TGCCCGACCGCAGGATCAAGTATGAGGCCAGGCTGCATGTGCTGAAGGTGAACCGTCAGACAGTACTAGA GGCTCTGCAGCAGCTGATAAGAGTAACACAGCCAGAGCTGATTCAGACCCACAAGTCTCAAGAGTCACAG CTGCCTGAGGAGTCCAAGTCAGCCAGCAACAAGTCCCCGCTGGTGCTGGAAGCAAACAGGGCCCCTGCAG CCTCTGAGGGCAACCACACAGATGGTGCAGAGGAGGCGGCTGGTTCATGCGCACAAGCCCCATCCCACAG CCCTCCCAACAAACCCAAGCTAGTGGTGAAGCCTCCAGGCAGCAGCCTCAATGGGGTTCACCCCAACCCC ACTCCCATTGTCCAGCGGCTGCCGGCCTTTCTAGACAATCACAATTATGCCAAGTCCCCCATGCAGGAGG AAGAAGACCTGGCGGCAGGTGTGGGCCGCAGCCGAGTTCCAGTCCGCCCACCCCAGCAGTACTCAGATGA TGAGGATGACTATGAGGATGACGAGGAGGATGACGTGCAGAACACCAACTCTGCCCTTAGGTATAAGGGG AAGGGAACAGGGAAGCCAGGGGCATTGAGCGGTTCTGCTGATGGGCAACTGTCAGTGCTGCAGCCCAACA CCATCAACGTCTTGGCTGAGAAGCTCAAAGAGTCCCAGAAGGACCTCTCAATTCCTCTGTCCATCAAGAC TAGCAGCGGGGCTGGGAGTCCGGCTGTGGCAGTGCCCACACACTCGCAGCCCTCACCCACCCCCAGCAAT GAGAGTACAGACACGGCCTCTGAGATCGGCAGTGCTTTCAACTCGCCACTGCGCTCGCCTATCCGCTCAG CCAACCCGACGCGGCCCTCCAGCCCTGTCACCTCCCACATCTCCAAGGTGCTTTTTGGAGAGGATGACAG CCTGCTGCGTGTTGACTGCATACGCTACAACCGTGCTGTCCGTGATCTGGGTCCTGTCATCAGCACAGGC CTGCTGCACCTGGCTGAGGATGGGGTGCTGAGTCCCCTGGCGCTGACAGAGGGTGGGAAGGGTTCCTCGC CCTCCATCAGACCAATCCAAGGCAGCCAGGGGTCCAGCAGCCCAGTGGAGAAGGAGGTCGTGGAAGCCAC GGACAGCAGAGAGAAGACGGGGATGGTGAGGCCTGGCGAGCCCTTGAGTGGGGAGAAATACTCACCCAAG GAGCTGCTGGCACTGCTGAAGTGTGTGGAGGCTGAGATTGCAAACTATGAGGCGTGCCTCAAGGAGGAGG TAGAGAAGAGGAAGAAGTTCAAGATTGATGACCAGAGAAGGACCCACAACTACGATGAGTTCATCTGCAC CTTTATCTCCATGCTGGCTCAGGAAGGCATGCTGGCCAACCTAGTGGAGCAGAACATCTCCGTGCGGCGG CGCCAAGGGGTCAGCATCGGCCGGCTCCACAAGCAGCGGAAGCCTGACCGGCGGAAACGCTCTCGCCCCT ACAAGGCCAAGCGCCAGTGA >OriGene 5' read for NM_004656 unedited
CCCCCCTATCTCCCGCCCGTTGCCGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTAT ATAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCG GCCGCGAATTCGGCACGAGGGGACTGAGGGGCCCCGGGGGCGGTGGGGGCTCCCGGTGGG GGCAGCGGTGGGGAGGGAGGGCCTGGACATGGCGCTGAGGGGCCGCCCCGCGGGAAGATG AATAAGGGCTGGCTGGAGCTGGAGAGCGACCCAGGCCTCTTCACCCTGCTCGTGGAAGAT TTCGGTGTCAAGGGGGTGCAAGTGGAGGAGATCTACGACCTTCAGAGCAAATGTCAGGGC CCTGTATATGGATTTATCTTCCTGTTCAAATGGATCGAAGAGCGCCGGTCCCGGCGAAAG GTCTCTACCTTGGTGGATGATACGTCCGTGATTGATGATGATATTGTGAATAACATGTTC TTTGCCCACCAGCTGATACCCAACTCTTGTGCAACTCATGCCTTGCTGAGCGTGCTCCTG AACTGCAGCAGCGTGGACCTGGGACCCACCCTGAGTCGCATGAAGGACTTCACCAAGGGT TTCAGCCCTGAGGCCCGAGCCACGCCACCTCCCTGAGAAGCAGAATGGCCTTAGTGCAGT GCGGACCATGGAGGCGTTCCACTTTGTCAGCTATGTGCCTATCACAGGCCGGCTCTATGA GCTGGATGGGCTGCAAGTCTACCCCATTGACCATGGGCCCTGGGGGGCAGACGAAGAGTG CACAGACCAAGCCCTGGCGGTCATCATGAAGCGTATCGGCCTCGCCACTGCAGGGGAGCC CTACCACGACATCCGCTTCAACCTGATGGCAGTGTTGCCCGACCGCAGGACCAAGTATGA GGCCTAGCTGCATT >OriGene 3' read for NM_004656 unedited
CAGGGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTCACGA AACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTCTTATCTGGGCCCTGACATTTGCT CTGAAGGTCGTAGATCTCCTCCACTTGCACCACCTCGACCGCGATTCTTAGGAGAGCTTG ATGCATTCGCTGGTCCCGTTTTTCCAGGGCCTACAGGGGTCAAGCTGTGCTCACCCCAAA GGCAGTTGCCACACTTGCCAGCCCCCCCCACTCAACCACTATGTCCACATAAAGGGGCCT GCTTGGATCACCTTTTTCAAAGCCATCTGGCCGAAGCCATGGGCCTTGGTTGGGGCCCGG GCTCCAAGGGCACTGTTGGGCCCATTACCCGTTGTCAGCAAGTCCTACGGAACACCGGGG CCCCAAACGGTCGATTTGCTCCTGCTGTCCCCCACCATGACTGCCTTGCAGAGGCTGCGG AAACTGGTGTTGCACGAACAGCCTACTGTTGCCATCTTCCTATAAGAGACCTTGTCTCTG CTCCCCGTGAGAGGGAGACATGGTCACACTGATGTGCTGGACAGAGGCTCTTATGATCCT AATGGCCCTGGCTCAGACACCTATGTCCCAATTTAGGCCCATAGCTCGGGCCGCCACGTT CAGGGCAAAGTGCCCTAGAACCTTGTTACGGAGAGCAGCCTTGATCAGCCTGGGACTAAC AAGAAAAAACGTTCAGAGGCCTGTGAACTGTTCCAACTACCTTTGCTACGGCCAAGGGAT CCCATCACCGTGGTTACCCATCGCCGTTGGGGACGCCAAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_004656 |
| Insert Size | 3520 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004656.2, NP_004647.1 |
| RefSeq Size | 3599 bp |
| RefSeq ORF | 2190 bp |
| Locus ID | 8314 |
| UniProt ID | Q92560 |
| Domains | Peptidase_C12 |
| Protein Families | Druggable Genome, Protease |
| Gene Summary | This gene belongs to the ubiquitin C-terminal hydrolase subfamily of deubiquitinating enzymes that are involved in the removal of ubiquitin from proteins. The encoded enzyme binds to the breast cancer type 1 susceptibility protein (BRCA1) via the RING finger domain of the latter and acts as a tumor suppressor. In addition, the enzyme may be involved in regulation of transcription, regulation of cell cycle and growth, response to DNA damage and chromatin dynamics. Germline mutations in this gene may be associated with tumor predisposition syndrome (TPDS), which involves increased risk of cancers including malignant mesothelioma, uveal melanoma and cutaneous melanoma. [provided by RefSeq, May 2013] |
Citations (4)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
BAP1 and YY1 regulate expression of death receptors in malignant pleural mesothelioma
,null,
The Journal of Biological Chemistry
,PubMed ID 34597666
[BAP1]
|
|
Loss of functional BAP1 augments sensitivity to TRAIL in cancer cells
,Kolluri, KK;Alifrangis, C;Kumar, N;Ishii, Y;Price, S;Michaut, M;Williams, S;Barthorpe, S;Lightfoot, H;Busacca, S;Sharkey, A;Yuan, Z;Sage, EK;Vallath, S;Le Quesne, J;Tice, DA;Alrifai, D;von Karstedt, S;Montinaro, A;Guppy, N;Waller, DA;Nakas, A;Good, R;Holmes, A;Walczak, H;Fennell, DA;Garnett, M;Iorio, F;Wessels, L;McDermott, U;Janes, SM;,
Elife
,PubMed ID 29345617
[BAP1]
|
|
BAP1 dependent expression of long non-coding RNA NEAT-1 contributes to sensitivity to gemcitabine in cholangiocarcinoma
,Parasramka, M;Yan, IK;Wang, X;Nguyen, P;Matsuda, A;Maji, S;Foye, C;Asmann, Y;Patel, T;,
Mol. Cancer
,PubMed ID 28122578
[BAP1]
|
|
Cancer-associated ASXL1 mutations may act as gain-of-function mutations of the ASXL1-BAP1 complex
,Balasubramani, A;Larjo, A;Bassein, JA;Chang, X;Hastie, RB;Togher, SM;Lähdesmäki, H;Rao, A;,
Nat Commun
,PubMed ID 26095772
[BAP1]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200378 | BAP1 (Myc-DDK-tagged)-Human BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase) (BAP1) |
CNY 5,320.00 |
|
| RC200378L1 | Lenti ORF clone of Human BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase) (BAP1), Myc-DDK-tagged |
CNY 10,408.00 |
|
| RC200378L2 | Lenti ORF clone of Human BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase) (BAP1), mGFP tagged |
CNY 10,408.00 |
|
| RC200378L3 | Lenti ORF clone of Human BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase) (BAP1), Myc-DDK-tagged |
CNY 10,408.00 |
|
| RC200378L4 | Lenti ORF clone of Human BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase) (BAP1), mGFP tagged |
CNY 10,408.00 |
|
| RG200378 | BAP1 (tGFP-tagged) - Human BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase) (BAP1) |
CNY 9,608.00 |
|
| SC320669 | BAP1 (untagged)-Human BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase) (BAP1) |
CNY 8,016.00 |
