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Factor XIIIa (F13A1) (NM_000129) Human Untagged Clone
CAT＃: SC120099
F13A1 (untagged)-Human coagulation factor XIII, A1 polypeptide (F13A1)
CNY 8,056.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | F13A |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC120099 sequence for NM_000129 edited (data generated by NextGen Sequencing)
ATGTCAGAAACTTCCAGGACCGCCTTTGGAGGCAGAAGAGCAGTTCCACCCAATAACTCT AATGCAGCGGAAGATGACCTGCCCACAGTGGAGCTTCAGGGCGTGGTGCCCCGGGGCGTC AACCTGCAAGAGTTTCTTAATGTCACGAGCGTTCACCTGTTCAAGGAGAGATGGGACACT AACAAGGTGGACCACCACACTGACAAGTATGAAAACAACAAGCTGATTGTCCGCAGAGGG CAGTCTTTCTATGTGCAGATTGACTTCAGTCGTCCATATGACCCCAGAAGGGATCTCTTC AGGGTGGAATACGTCATTGGTCGCTACCCACAGGAGAACAAGGGAACCTACATCCCAGTG CCTATAGTCTCAGAGTTACAAAGTGGAAAGTGGGGGGCCAAGATTGTCATGAGAGAGGAC AGGTCTGTGCGGCTGTCCATCCAGTCTTCCCCCAAATGTATTGTGGGGAAATTCCGCATG TATGTTGCTGTCTGGACTCCCTATGGCGTACTTCGAACCAGTCGAAACCCAGAAACAGAC ACGTACATTCTCTTCAATCCTTGGTGTGAAGATGATGCTGTGTATCTGGACAATGAGAAA GAAAGAGAAGAGTATGTCCTGAATGACATCGGGGTAATTTTTTATGGAGAGGTCAATGAC ATCAAGACCAGAAGCTGGAGCTATGGTCAGTTTGAAGATGGCATCCTGGACACTTGCCTG TATGTGATGGACAGAGCACAAATGGACCTCTCTGGAAGAGGGAATCCCATCAAAGTCAGC CGTGTGGGGTCTGCAATGGTGAATGCCAAAGATGACGAAGGTGTCCTCGTTGGATCCTGG GACAATATCTATGCCTATGGCGTCCCCCCATCGGCCTGGACTGGAAGCGTTGACATTCTA TTGGAATACCGGAGCTCTGAGAATCCAGTCCGGTATGGCCAATGCTGGGTTTTTGCTGGT GTCTTTAACACATTTTTACGATGCCTTGGAATACCAGCAAGAATTGTTACCAATTATTTC TCTGCCCATGATAATGATGCCAATTTGCAAATGGACATCTTCCTGGAAGAAGATGGGAAC GTGAATTCCAAACTCACCAAGGATTCAGTGTGGAACTACCACTGCTGGAATGAAGCATGG ATGACAAGGCCTGACCTTCCTGTTGGATTTGGAGGCTGGCAAGCTGTGGACAGCACCCCC CAGGAAAATAGCGATGGCATGTATCGGTGTGGCCCCGCCTCGGTTCAAGCCATCAAGCAC GGCCATGTCTGCTTCCAATTTGATGCACCTTTTGTTTTTGCAGAGGTCAACAGCGACCTC ATTTACATTACAGCTAAGAAAGATGGCACTCATGTGGTGGAAAATGTGGATGCCACCCAC ATTGGGAAATTAATTGTGACCAAACAAATTGGAGGAGATGGCATGATGGATATTACTGAT ACTTACAAATTCCAAGAAGGTCAAGAAGAAGAGAGATTGGCCCTAGAAACTGCCCTGATG TACGGAGCTAAAAAGCCCCTCAACACAGAAGGTGTCATGAAATCAAGGTCCAACGTTGAC ATGGACTTTGAAGTGGAAAATGCTGTGCTGGGAAAAGACTTCAAGCTCTCCATCACCTTC CGGAACAACAGCCACAACCGTTACACCATCACAGCTTATCTCTCAGCCAACATCACCTTC TACACCGGGGTCCCGAAGGCAGAATTCAAGAAGGAGACGTTCGACGTGACGCTGGAGCCC TTGTCCTTCAAGAAAGAGGCGGTGCTGATCCAAGCCGGCGAGTACATGGGTCAGCTGCTG GAACAAGCGTCCCTGCACTTCTTTGTCACAGCTCGCATCAATGAGACCAGGGATGTTCTG GCCAAGCAAAAGTCCACCGTGCTAACCATCCCTGAGATCATCATCAAGGTCCGTGGCACT CAGGTAGTTGGTTCTGACATGACTGTGACAGTTCAGTTTACCAATCCTTTAAAAGAAACC CTGCGAAATGTCTGGGTACACCTGGATGGTCCTGGAGTAACAAGACCAATGAAGAAGATG TTCCGTGAAATCCGGCCCAACTCCACCGTGCAGTGGGAAGAAGTGTGCCGGCCCTGGGTC TCTGGGCATCGGAAGCTGATAGCCAGCATGAGCAGTGACTCCCTGAGACATGTGTATGGC GAGCTGGACGTGCAGATTCAAAGACGACCTTCCATGTGA Clone variation with respect to NM_000129.3 1954 g=>c >OriGene 5' read for NM_000129 unedited
GATTTTGTAATACGACTTTACTATANNGGCGGCCGCGCAATTCGGCACGAGGCCGAGGCG CACAGAGCAAGCCCACGCGAGGGCACCTCTGGAGGGGAGCGCCTGCAGGACCTTGTAAAG TCAAAAATGTCAGAAACTTCCAGGACCGCCTTTGGAGGCAGAAGAGCAGTTCCACCCAAT AACTCTAATGCAGCGGAAGATGACCTGCCCACAGTGGAGCTTCAGGGCGTGGTGCCCCGG GGCGTCAACCTGCAAGAGTTTCTTAATGTCACGAGCGTTCACCTGTTCAAGGAGAGATGG GACACTAACAAGGTGGACCACCACACTGACAAGTATGAAAACAACAAGCTGATTGTCCGC AGAGGGCAGTCTTTCTATGTGCAGATTGACTTCAGTCGTCCATATGACCCCAGAAGGGAT CTCTTCAGGGTGGAATACGTCATTGGTCGCTACCCACAGGAGAACAAGGGAACCTACATC CCAGTGCCTATAGTCTCAGAGTTACAAAGTGGAAAGTGGGGGGCCAAGATTGTCATGAGA GAGGACAGGTCTGTGCGGCTGTCCATCCAGTCTTCCCCCAAATGTATTGTGGGGAAATTC CGCATGTATGTTGCTGTCTGGACTCCCTATGGCGTACTTCGAACCAGTCGAAACCCAGAA ACAGACACGTACATTCTCTTCAATCCTTGGTGTGAAGATGATGCTGTGTATCTGGACCAT GAGAAAGAAAGAGAAGAGTATGTCCTGAATGACATCGGNGTAATTNTTTATGGAGAGGTC AATGACATCAAGACCAGAAGCTGGAGCTATGGTCAGTTTGAAGATGGCATCCTGGACACT TGCCTGTATGTGATGGACAGAGCACAAATGGACCTCTCTGGNAGAGGGAATCCATCAAGT CAGCCGTGTGGGGTCTGCAATGGTGAATGCAAAGAGACGAAGGTGNCTCCGTGGAACCTG GGACATATCTTGCCTT >OriGene 3' read for NM_000129 unedited
NNNNTTTTGACTTTGGACCGGCGGCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTTT ATAATCAGTTTAACTTTATTTTAAGCTTCTATTACATTGTAATTAATAATAATATACTAA GTTAAGCCAGTTTAACTGTCTAGCAAAATGCATTTCATTTTAAAATTGGATATTGGGAGA CTTGGCAAATGCTGTGAGATTACTTAGTAAAGTTAAGTATGATGTATATATAGAGGGGAC CAGCTCACCCTCATAGGTTAGTGCTGAAGGCTCAGGAACAGTCTCAAAGGCTTAGAAATG TGGTTATATAGACCAGAGCATTCCATTCTGATTTTGCCCCCAGTATACTTATCACTGTTC ATTTTTTGCTAAGAATCACAGTCTACTGACCTAAATCACACCCTAGACATATCAGAGGGA AATTCTGACCATAAATCAGCCTTGCAAATACATAGCAGGCAGCCTTCTCTGTAGTAGCCT TGGAACATAATACAAATGATGTCAGCATCCTCTGTGGTTGGGTCAGAACTAGCACTGGCT ATTTGCAAGGCTGAGTCCATATTACCCTTCAGCACCTGAGTGCCAAGCTGAGGTCTCAAA ATGGGTGATGTGAAGGACAAGAGAGAATCAGTGGCTGCAGTCCTTCTATATATCTGGTCA CTAGATCCGCCAGCTTCTTCAACTTGTTGGGCTTATTATATCTCTGAAAGGGGACCTGAA CCTGCATCATTCTGCAAGGAGATGATTCCTGAGAAATGGGTGAAATATATCACTAACTAA AGAGATAATTCTTCACTTTACTTCCTAGTATTGCATNCCAAAAGGGNTAAAGTAGGGGAT ATTGGCTCCTGCTGATCGGNCCACAGTGTGACCCATCTATAAATGCATCACAAACTACAT ATTC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000129 |
| Insert Size | 3870 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000129.2, NP_000120.1 |
| RefSeq Size | 3833 bp |
| RefSeq ORF | 2199 bp |
| Locus ID | 2162 |
| UniProt ID | P00488 |
| Domains | Transglutamin_C, TGc |
| Protein Families | Druggable Genome, Secreted Protein |
| Protein Pathways | Complement and coagulation cascades |
| Gene Summary | This gene encodes the coagulation factor XIII A subunit. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as plasma carrier molecules. Platelet factor XIII is comprised only of 2 A subunits, which are identical to those of plasma origin. Upon cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIIIa, as platelet factor XIII. This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. It also crosslinks alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206464 | F13A1 (Myc-DDK-tagged)-Human coagulation factor XIII, A1 polypeptide (F13A1) |
CNY 8,040.00 |
|
| RC206464L1 | Lenti ORF clone of Human coagulation factor XIII, A1 polypeptide (F13A1), Myc-DDK-tagged |
CNY 10,440.00 |
|
| RC206464L2 | Lenti ORF clone of Human coagulation factor XIII, A1 polypeptide (F13A1), mGFP tagged |
CNY 5,890.00 |
|
| RC206464L3 | Lenti ORF clone of Human coagulation factor XIII, A1 polypeptide (F13A1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206464L4 | Lenti ORF clone of Human coagulation factor XIII, A1 polypeptide (F13A1), mGFP tagged |
CNY 5,890.00 |
|
| RG206464 | F13A1 (tGFP-tagged) - Human coagulation factor XIII, A1 polypeptide (F13A1) |
CNY 9,640.00 |
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